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Adsorption of Cationic Peptides to Solid Surfaces of Glass and Plastic
Cationic membrane-active peptides have been studied for years in the hope of developing them into novel types of therapeutics. In this article, we investigate an effect that might have significant experimental implications for investigators who wish to study these peptides, namely, that the peptides...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4416745/ https://www.ncbi.nlm.nih.gov/pubmed/25932639 http://dx.doi.org/10.1371/journal.pone.0122419 |
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author | Kristensen, Kasper Henriksen, Jonas R. Andresen, Thomas L. |
author_facet | Kristensen, Kasper Henriksen, Jonas R. Andresen, Thomas L. |
author_sort | Kristensen, Kasper |
collection | PubMed |
description | Cationic membrane-active peptides have been studied for years in the hope of developing them into novel types of therapeutics. In this article, we investigate an effect that might have significant experimental implications for investigators who wish to study these peptides, namely, that the peptides adsorb to solid surfaces of glass and plastic. Specifically, we use analytical HPLC to systematically quantify the adsorption of the three cationic membrane-active peptides mastoparan X, melittin, and magainin 2 to the walls of commonly used glass and plastic sample containers. Our results show that, at typical experimental peptide concentrations, 90% or more of the peptides might be lost from solution due to rapid adsorption to the walls of the sample containers. Thus, our results emphasize that investigators should always keep these adsorption effects in mind when designing and interpreting experiments on cationic membrane-active peptides. We conclude the article by discussing different strategies for reducing the experimental impact of these adsorption effects. |
format | Online Article Text |
id | pubmed-4416745 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-44167452015-05-07 Adsorption of Cationic Peptides to Solid Surfaces of Glass and Plastic Kristensen, Kasper Henriksen, Jonas R. Andresen, Thomas L. PLoS One Research Article Cationic membrane-active peptides have been studied for years in the hope of developing them into novel types of therapeutics. In this article, we investigate an effect that might have significant experimental implications for investigators who wish to study these peptides, namely, that the peptides adsorb to solid surfaces of glass and plastic. Specifically, we use analytical HPLC to systematically quantify the adsorption of the three cationic membrane-active peptides mastoparan X, melittin, and magainin 2 to the walls of commonly used glass and plastic sample containers. Our results show that, at typical experimental peptide concentrations, 90% or more of the peptides might be lost from solution due to rapid adsorption to the walls of the sample containers. Thus, our results emphasize that investigators should always keep these adsorption effects in mind when designing and interpreting experiments on cationic membrane-active peptides. We conclude the article by discussing different strategies for reducing the experimental impact of these adsorption effects. Public Library of Science 2015-05-01 /pmc/articles/PMC4416745/ /pubmed/25932639 http://dx.doi.org/10.1371/journal.pone.0122419 Text en © 2015 Kristensen et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Kristensen, Kasper Henriksen, Jonas R. Andresen, Thomas L. Adsorption of Cationic Peptides to Solid Surfaces of Glass and Plastic |
title | Adsorption of Cationic Peptides to Solid Surfaces of Glass and Plastic |
title_full | Adsorption of Cationic Peptides to Solid Surfaces of Glass and Plastic |
title_fullStr | Adsorption of Cationic Peptides to Solid Surfaces of Glass and Plastic |
title_full_unstemmed | Adsorption of Cationic Peptides to Solid Surfaces of Glass and Plastic |
title_short | Adsorption of Cationic Peptides to Solid Surfaces of Glass and Plastic |
title_sort | adsorption of cationic peptides to solid surfaces of glass and plastic |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4416745/ https://www.ncbi.nlm.nih.gov/pubmed/25932639 http://dx.doi.org/10.1371/journal.pone.0122419 |
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