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Ascaris and Escherichia coli Inactivation in an Ecological Sanitation System in Port-au-Prince, Haiti
The goal of this study was to evaluate the microbial die-off in a latrine waste composting system in Port-au-Prince, Haiti. Temperature data and samples were collected from compost aged 0 – 12+ months. Samples collected from compost bin centers and corners at two depths were assessed for moisture co...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4416818/ https://www.ncbi.nlm.nih.gov/pubmed/25932948 http://dx.doi.org/10.1371/journal.pone.0125336 |
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author | Berendes, David Levy, Karen Knee, Jackie Handzel, Thomas Hill, Vincent R. |
author_facet | Berendes, David Levy, Karen Knee, Jackie Handzel, Thomas Hill, Vincent R. |
author_sort | Berendes, David |
collection | PubMed |
description | The goal of this study was to evaluate the microbial die-off in a latrine waste composting system in Port-au-Prince, Haiti. Temperature data and samples were collected from compost aged 0 – 12+ months. Samples collected from compost bin centers and corners at two depths were assessed for moisture content, E. coli concentration, and Ascaris spp. viability. Center temperatures in compost bins were all above 58 °C, while corner temperatures were 10 – 20 °C lower. Moisture content was 67 ± 10% in all except the oldest compost. A 4-log reduction in E. coli was observed over the first sixteen weeks of composting at both locations and depths, after which E. coli was undetectable (LOD: 142 MPN g(-1) dry weight). In new compost, 10.4% and 8.3% of Ascaris eggs were viable and fully embryonated, respectively. Percent viability dropped to zero in samples older than six weeks. These findings indicate that the Haitian EcoSan composting process was effective in inactivating E. coli and Ascaris spp. in latrine waste within sixteen weeks. This study is one of the first to document efficacy of an ecological sanitation system under field conditions and provides insight into composting methods and monitoring for other international settings. |
format | Online Article Text |
id | pubmed-4416818 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-44168182015-05-07 Ascaris and Escherichia coli Inactivation in an Ecological Sanitation System in Port-au-Prince, Haiti Berendes, David Levy, Karen Knee, Jackie Handzel, Thomas Hill, Vincent R. PLoS One Research Article The goal of this study was to evaluate the microbial die-off in a latrine waste composting system in Port-au-Prince, Haiti. Temperature data and samples were collected from compost aged 0 – 12+ months. Samples collected from compost bin centers and corners at two depths were assessed for moisture content, E. coli concentration, and Ascaris spp. viability. Center temperatures in compost bins were all above 58 °C, while corner temperatures were 10 – 20 °C lower. Moisture content was 67 ± 10% in all except the oldest compost. A 4-log reduction in E. coli was observed over the first sixteen weeks of composting at both locations and depths, after which E. coli was undetectable (LOD: 142 MPN g(-1) dry weight). In new compost, 10.4% and 8.3% of Ascaris eggs were viable and fully embryonated, respectively. Percent viability dropped to zero in samples older than six weeks. These findings indicate that the Haitian EcoSan composting process was effective in inactivating E. coli and Ascaris spp. in latrine waste within sixteen weeks. This study is one of the first to document efficacy of an ecological sanitation system under field conditions and provides insight into composting methods and monitoring for other international settings. Public Library of Science 2015-05-01 /pmc/articles/PMC4416818/ /pubmed/25932948 http://dx.doi.org/10.1371/journal.pone.0125336 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. |
spellingShingle | Research Article Berendes, David Levy, Karen Knee, Jackie Handzel, Thomas Hill, Vincent R. Ascaris and Escherichia coli Inactivation in an Ecological Sanitation System in Port-au-Prince, Haiti |
title |
Ascaris and Escherichia coli Inactivation in an Ecological Sanitation System in Port-au-Prince, Haiti |
title_full |
Ascaris and Escherichia coli Inactivation in an Ecological Sanitation System in Port-au-Prince, Haiti |
title_fullStr |
Ascaris and Escherichia coli Inactivation in an Ecological Sanitation System in Port-au-Prince, Haiti |
title_full_unstemmed |
Ascaris and Escherichia coli Inactivation in an Ecological Sanitation System in Port-au-Prince, Haiti |
title_short |
Ascaris and Escherichia coli Inactivation in an Ecological Sanitation System in Port-au-Prince, Haiti |
title_sort | ascaris and escherichia coli inactivation in an ecological sanitation system in port-au-prince, haiti |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4416818/ https://www.ncbi.nlm.nih.gov/pubmed/25932948 http://dx.doi.org/10.1371/journal.pone.0125336 |
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