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Polyethylene Oxide (PEO) and Polyethylene Glycol (PEG) Polymer Sieving Matrix for RNA Capillary Electrophoresis

The selection of sieving polymer for RNA fragments separation by capillary electrophoresis is imperative. We investigated the separation of RNA fragments ranged from 100 to 10,000 nt in polyethylene glycol (PEG) and polyethylene oxide (PEO) solutions with different molecular weight and different con...

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Detalles Bibliográficos
Autores principales: Yamaguchi, Yoshinori, Li, Zhenqing, Zhu, Xifang, Liu, Chenchen, Zhang, Dawei, Dou, Xiaoming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4416823/
https://www.ncbi.nlm.nih.gov/pubmed/25933347
http://dx.doi.org/10.1371/journal.pone.0123406
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author Yamaguchi, Yoshinori
Li, Zhenqing
Zhu, Xifang
Liu, Chenchen
Zhang, Dawei
Dou, Xiaoming
author_facet Yamaguchi, Yoshinori
Li, Zhenqing
Zhu, Xifang
Liu, Chenchen
Zhang, Dawei
Dou, Xiaoming
author_sort Yamaguchi, Yoshinori
collection PubMed
description The selection of sieving polymer for RNA fragments separation by capillary electrophoresis is imperative. We investigated the separation of RNA fragments ranged from 100 to 10,000 nt in polyethylene glycol (PEG) and polyethylene oxide (PEO) solutions with different molecular weight and different concentration. We found that the separation performance of the small RNA fragments (<1000 nt) was improved with the increase of polymer concentration, whereas the separation performance for the large ones (>4000 nt) deteriorated in PEG/PEO solutions when the concentration was above 1.0%/0.6%, respectively. By double logarithmic plot of mobility and RNA fragment size, we revealed three migration regimes for RNA in PEG (300-500k) and PEO (4,000k). Moreover, we calculated the smallest resolvable nucleotide length (N (min)) from the resolution length analysis.
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spelling pubmed-44168232015-05-07 Polyethylene Oxide (PEO) and Polyethylene Glycol (PEG) Polymer Sieving Matrix for RNA Capillary Electrophoresis Yamaguchi, Yoshinori Li, Zhenqing Zhu, Xifang Liu, Chenchen Zhang, Dawei Dou, Xiaoming PLoS One Research Article The selection of sieving polymer for RNA fragments separation by capillary electrophoresis is imperative. We investigated the separation of RNA fragments ranged from 100 to 10,000 nt in polyethylene glycol (PEG) and polyethylene oxide (PEO) solutions with different molecular weight and different concentration. We found that the separation performance of the small RNA fragments (<1000 nt) was improved with the increase of polymer concentration, whereas the separation performance for the large ones (>4000 nt) deteriorated in PEG/PEO solutions when the concentration was above 1.0%/0.6%, respectively. By double logarithmic plot of mobility and RNA fragment size, we revealed three migration regimes for RNA in PEG (300-500k) and PEO (4,000k). Moreover, we calculated the smallest resolvable nucleotide length (N (min)) from the resolution length analysis. Public Library of Science 2015-05-01 /pmc/articles/PMC4416823/ /pubmed/25933347 http://dx.doi.org/10.1371/journal.pone.0123406 Text en © 2015 Yamaguchi et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Yamaguchi, Yoshinori
Li, Zhenqing
Zhu, Xifang
Liu, Chenchen
Zhang, Dawei
Dou, Xiaoming
Polyethylene Oxide (PEO) and Polyethylene Glycol (PEG) Polymer Sieving Matrix for RNA Capillary Electrophoresis
title Polyethylene Oxide (PEO) and Polyethylene Glycol (PEG) Polymer Sieving Matrix for RNA Capillary Electrophoresis
title_full Polyethylene Oxide (PEO) and Polyethylene Glycol (PEG) Polymer Sieving Matrix for RNA Capillary Electrophoresis
title_fullStr Polyethylene Oxide (PEO) and Polyethylene Glycol (PEG) Polymer Sieving Matrix for RNA Capillary Electrophoresis
title_full_unstemmed Polyethylene Oxide (PEO) and Polyethylene Glycol (PEG) Polymer Sieving Matrix for RNA Capillary Electrophoresis
title_short Polyethylene Oxide (PEO) and Polyethylene Glycol (PEG) Polymer Sieving Matrix for RNA Capillary Electrophoresis
title_sort polyethylene oxide (peo) and polyethylene glycol (peg) polymer sieving matrix for rna capillary electrophoresis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4416823/
https://www.ncbi.nlm.nih.gov/pubmed/25933347
http://dx.doi.org/10.1371/journal.pone.0123406
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