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Polyethylene Oxide (PEO) and Polyethylene Glycol (PEG) Polymer Sieving Matrix for RNA Capillary Electrophoresis
The selection of sieving polymer for RNA fragments separation by capillary electrophoresis is imperative. We investigated the separation of RNA fragments ranged from 100 to 10,000 nt in polyethylene glycol (PEG) and polyethylene oxide (PEO) solutions with different molecular weight and different con...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4416823/ https://www.ncbi.nlm.nih.gov/pubmed/25933347 http://dx.doi.org/10.1371/journal.pone.0123406 |
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author | Yamaguchi, Yoshinori Li, Zhenqing Zhu, Xifang Liu, Chenchen Zhang, Dawei Dou, Xiaoming |
author_facet | Yamaguchi, Yoshinori Li, Zhenqing Zhu, Xifang Liu, Chenchen Zhang, Dawei Dou, Xiaoming |
author_sort | Yamaguchi, Yoshinori |
collection | PubMed |
description | The selection of sieving polymer for RNA fragments separation by capillary electrophoresis is imperative. We investigated the separation of RNA fragments ranged from 100 to 10,000 nt in polyethylene glycol (PEG) and polyethylene oxide (PEO) solutions with different molecular weight and different concentration. We found that the separation performance of the small RNA fragments (<1000 nt) was improved with the increase of polymer concentration, whereas the separation performance for the large ones (>4000 nt) deteriorated in PEG/PEO solutions when the concentration was above 1.0%/0.6%, respectively. By double logarithmic plot of mobility and RNA fragment size, we revealed three migration regimes for RNA in PEG (300-500k) and PEO (4,000k). Moreover, we calculated the smallest resolvable nucleotide length (N (min)) from the resolution length analysis. |
format | Online Article Text |
id | pubmed-4416823 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-44168232015-05-07 Polyethylene Oxide (PEO) and Polyethylene Glycol (PEG) Polymer Sieving Matrix for RNA Capillary Electrophoresis Yamaguchi, Yoshinori Li, Zhenqing Zhu, Xifang Liu, Chenchen Zhang, Dawei Dou, Xiaoming PLoS One Research Article The selection of sieving polymer for RNA fragments separation by capillary electrophoresis is imperative. We investigated the separation of RNA fragments ranged from 100 to 10,000 nt in polyethylene glycol (PEG) and polyethylene oxide (PEO) solutions with different molecular weight and different concentration. We found that the separation performance of the small RNA fragments (<1000 nt) was improved with the increase of polymer concentration, whereas the separation performance for the large ones (>4000 nt) deteriorated in PEG/PEO solutions when the concentration was above 1.0%/0.6%, respectively. By double logarithmic plot of mobility and RNA fragment size, we revealed three migration regimes for RNA in PEG (300-500k) and PEO (4,000k). Moreover, we calculated the smallest resolvable nucleotide length (N (min)) from the resolution length analysis. Public Library of Science 2015-05-01 /pmc/articles/PMC4416823/ /pubmed/25933347 http://dx.doi.org/10.1371/journal.pone.0123406 Text en © 2015 Yamaguchi et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Yamaguchi, Yoshinori Li, Zhenqing Zhu, Xifang Liu, Chenchen Zhang, Dawei Dou, Xiaoming Polyethylene Oxide (PEO) and Polyethylene Glycol (PEG) Polymer Sieving Matrix for RNA Capillary Electrophoresis |
title | Polyethylene Oxide (PEO) and Polyethylene Glycol (PEG) Polymer Sieving Matrix for RNA Capillary Electrophoresis |
title_full | Polyethylene Oxide (PEO) and Polyethylene Glycol (PEG) Polymer Sieving Matrix for RNA Capillary Electrophoresis |
title_fullStr | Polyethylene Oxide (PEO) and Polyethylene Glycol (PEG) Polymer Sieving Matrix for RNA Capillary Electrophoresis |
title_full_unstemmed | Polyethylene Oxide (PEO) and Polyethylene Glycol (PEG) Polymer Sieving Matrix for RNA Capillary Electrophoresis |
title_short | Polyethylene Oxide (PEO) and Polyethylene Glycol (PEG) Polymer Sieving Matrix for RNA Capillary Electrophoresis |
title_sort | polyethylene oxide (peo) and polyethylene glycol (peg) polymer sieving matrix for rna capillary electrophoresis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4416823/ https://www.ncbi.nlm.nih.gov/pubmed/25933347 http://dx.doi.org/10.1371/journal.pone.0123406 |
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