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Prohibitin Is Involved in Patients with IgG4 Related Disease

OBJECTIVE: IgG4-related disease (IgG4-RD) is a chronic systemic disease involved in many organs and tissues. As only limited autoantigens have been found since the beginning of this century, the aim of this study was to reveal new candidate autoantigens of IgG4-RD. METHODS: Multiple cell lines inclu...

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Autores principales: Du, Hongwu, Shi, Lili, Chen, Peng, Yang, Weikang, Xun, Yiping, Yang, Chunhe, Zhao, Lanqing, Zhou, Yabin, Chen, Guangyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4416882/
https://www.ncbi.nlm.nih.gov/pubmed/25932630
http://dx.doi.org/10.1371/journal.pone.0125331
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author Du, Hongwu
Shi, Lili
Chen, Peng
Yang, Weikang
Xun, Yiping
Yang, Chunhe
Zhao, Lanqing
Zhou, Yabin
Chen, Guangyu
author_facet Du, Hongwu
Shi, Lili
Chen, Peng
Yang, Weikang
Xun, Yiping
Yang, Chunhe
Zhao, Lanqing
Zhou, Yabin
Chen, Guangyu
author_sort Du, Hongwu
collection PubMed
description OBJECTIVE: IgG4-related disease (IgG4-RD) is a chronic systemic disease involved in many organs and tissues. As only limited autoantigens have been found since the beginning of this century, the aim of this study was to reveal new candidate autoantigens of IgG4-RD. METHODS: Multiple cell lines including HT-29, EA.hy926, HEK 293 and HepG2 were used to test the binding ability of circulating autoantibodies from IgG4-RD sera. The amino-acid sequence was then analyzed by matrix-assisted laser desorption/ionization time-of-flight tandem (MALDI-TOF/TOF) mass spectrometry. After the cloning and expression of recombinant putative autoantigen in a bacterial expression system, the corresponding immuno assay was set up and utilized to observe the prevalence of serum autoantibodies in a large set of confirmed clinical samples. RESULTS: One positive autoantigen was identified as prohibitin. ELISA analysis showed that a majority of patients with IgG4-RD have antibodies against prohibitin. Anti-prohibitin antibodies were present in the sera of patients with definite autoimmune pancreatitis (25/34; 73.5%), Mikulicz’s disease (8/15; 53.3%), retroperitoneal fibrosis (6/11; 54.5%), other probable IgG4-RD (26/29; 89.7%) and Sjögren’s syndrome (4/30; 13.3%) but not in apparently healthy donors (1/70; 1.4%). CONCLUSIONS: An association between prohibitin and patients with some IgG4-RD was observed, although the results were quite heterogeneous among different individuals within autoimmune pancreatitis, Mikulicz’s disease and retroperitoneal fibrosis.
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spelling pubmed-44168822015-05-07 Prohibitin Is Involved in Patients with IgG4 Related Disease Du, Hongwu Shi, Lili Chen, Peng Yang, Weikang Xun, Yiping Yang, Chunhe Zhao, Lanqing Zhou, Yabin Chen, Guangyu PLoS One Research Article OBJECTIVE: IgG4-related disease (IgG4-RD) is a chronic systemic disease involved in many organs and tissues. As only limited autoantigens have been found since the beginning of this century, the aim of this study was to reveal new candidate autoantigens of IgG4-RD. METHODS: Multiple cell lines including HT-29, EA.hy926, HEK 293 and HepG2 were used to test the binding ability of circulating autoantibodies from IgG4-RD sera. The amino-acid sequence was then analyzed by matrix-assisted laser desorption/ionization time-of-flight tandem (MALDI-TOF/TOF) mass spectrometry. After the cloning and expression of recombinant putative autoantigen in a bacterial expression system, the corresponding immuno assay was set up and utilized to observe the prevalence of serum autoantibodies in a large set of confirmed clinical samples. RESULTS: One positive autoantigen was identified as prohibitin. ELISA analysis showed that a majority of patients with IgG4-RD have antibodies against prohibitin. Anti-prohibitin antibodies were present in the sera of patients with definite autoimmune pancreatitis (25/34; 73.5%), Mikulicz’s disease (8/15; 53.3%), retroperitoneal fibrosis (6/11; 54.5%), other probable IgG4-RD (26/29; 89.7%) and Sjögren’s syndrome (4/30; 13.3%) but not in apparently healthy donors (1/70; 1.4%). CONCLUSIONS: An association between prohibitin and patients with some IgG4-RD was observed, although the results were quite heterogeneous among different individuals within autoimmune pancreatitis, Mikulicz’s disease and retroperitoneal fibrosis. Public Library of Science 2015-05-01 /pmc/articles/PMC4416882/ /pubmed/25932630 http://dx.doi.org/10.1371/journal.pone.0125331 Text en © 2015 Du et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Du, Hongwu
Shi, Lili
Chen, Peng
Yang, Weikang
Xun, Yiping
Yang, Chunhe
Zhao, Lanqing
Zhou, Yabin
Chen, Guangyu
Prohibitin Is Involved in Patients with IgG4 Related Disease
title Prohibitin Is Involved in Patients with IgG4 Related Disease
title_full Prohibitin Is Involved in Patients with IgG4 Related Disease
title_fullStr Prohibitin Is Involved in Patients with IgG4 Related Disease
title_full_unstemmed Prohibitin Is Involved in Patients with IgG4 Related Disease
title_short Prohibitin Is Involved in Patients with IgG4 Related Disease
title_sort prohibitin is involved in patients with igg4 related disease
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4416882/
https://www.ncbi.nlm.nih.gov/pubmed/25932630
http://dx.doi.org/10.1371/journal.pone.0125331
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