Articular Joint Lubricants during Osteoarthritis and Rheumatoid Arthritis Display Altered Levels and Molecular Species

BACKGROUND: Hyaluronic acid (HA), lubricin, and phospholipid species (PLs) contribute independently or together to the boundary lubrication of articular joints that is provided by synovial fluid (SF). Our study is the first reporting quantitative data about the molecular weight (MW) forms of HA, lub...

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Autores principales: Kosinska, Marta Krystyna, Ludwig, Taryn E., Liebisch, Gerhard, Zhang, Ruiyan, Siebert, Hans-Christian, Wilhelm, Jochen, Kaesser, Ulrich, Dettmeyer, Reinhard B., Klein, Heiko, Ishaque, Bernd, Rickert, Markus, Schmitz, Gerd, Schmidt, Tannin A., Steinmeyer, Juergen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4416892/
https://www.ncbi.nlm.nih.gov/pubmed/25933137
http://dx.doi.org/10.1371/journal.pone.0125192
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author Kosinska, Marta Krystyna
Ludwig, Taryn E.
Liebisch, Gerhard
Zhang, Ruiyan
Siebert, Hans-Christian
Wilhelm, Jochen
Kaesser, Ulrich
Dettmeyer, Reinhard B.
Klein, Heiko
Ishaque, Bernd
Rickert, Markus
Schmitz, Gerd
Schmidt, Tannin A.
Steinmeyer, Juergen
author_facet Kosinska, Marta Krystyna
Ludwig, Taryn E.
Liebisch, Gerhard
Zhang, Ruiyan
Siebert, Hans-Christian
Wilhelm, Jochen
Kaesser, Ulrich
Dettmeyer, Reinhard B.
Klein, Heiko
Ishaque, Bernd
Rickert, Markus
Schmitz, Gerd
Schmidt, Tannin A.
Steinmeyer, Juergen
author_sort Kosinska, Marta Krystyna
collection PubMed
description BACKGROUND: Hyaluronic acid (HA), lubricin, and phospholipid species (PLs) contribute independently or together to the boundary lubrication of articular joints that is provided by synovial fluid (SF). Our study is the first reporting quantitative data about the molecular weight (MW) forms of HA, lubricin, and PLs in SF from cohorts of healthy donors, patients with early (eOA)- or late (lOA)-stage osteoarthritis (OA), and patients with active rheumatoid arthritis (RA). METHODS: We used human SF from unaffected controls, eOA, lOA, and RA. HA and lubricin levels were measured by enzyme-linked immunosorbent assay. PLs was quantified by electrospray ionization tandem mass spectrometry. Fatty acids (FAs) were analyzed by gas chromatography, coupled with mass spectrometry. The MW distribution of HA was determined by agarose gel electrophoresis. RESULTS: Compared with control SF, the concentrations of HA and lubricin were lower in OA and RA SF, whereas those of PLs were higher in OA and RA SF. Moreover, the MW distribution of HA shifted toward the lower ranges in OA and RA SF. We noted distinct alterations between cohorts in the relative distribution of PLs and the degree of FA saturation and chain lengths of FAs. CONCLUSIONS: The levels, composition, and MW distribution of all currently known lubricants in SF—HA, lubricin, PLs—vary with joint disease and stage of OA. Our study is the first delivering a comprehensive view about all joint lubricants during health and widespread joint diseases. Thus, we provide the framework to develop new optimal compounded lubricants to reduce joint destruction.
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spelling pubmed-44168922015-05-07 Articular Joint Lubricants during Osteoarthritis and Rheumatoid Arthritis Display Altered Levels and Molecular Species Kosinska, Marta Krystyna Ludwig, Taryn E. Liebisch, Gerhard Zhang, Ruiyan Siebert, Hans-Christian Wilhelm, Jochen Kaesser, Ulrich Dettmeyer, Reinhard B. Klein, Heiko Ishaque, Bernd Rickert, Markus Schmitz, Gerd Schmidt, Tannin A. Steinmeyer, Juergen PLoS One Research Article BACKGROUND: Hyaluronic acid (HA), lubricin, and phospholipid species (PLs) contribute independently or together to the boundary lubrication of articular joints that is provided by synovial fluid (SF). Our study is the first reporting quantitative data about the molecular weight (MW) forms of HA, lubricin, and PLs in SF from cohorts of healthy donors, patients with early (eOA)- or late (lOA)-stage osteoarthritis (OA), and patients with active rheumatoid arthritis (RA). METHODS: We used human SF from unaffected controls, eOA, lOA, and RA. HA and lubricin levels were measured by enzyme-linked immunosorbent assay. PLs was quantified by electrospray ionization tandem mass spectrometry. Fatty acids (FAs) were analyzed by gas chromatography, coupled with mass spectrometry. The MW distribution of HA was determined by agarose gel electrophoresis. RESULTS: Compared with control SF, the concentrations of HA and lubricin were lower in OA and RA SF, whereas those of PLs were higher in OA and RA SF. Moreover, the MW distribution of HA shifted toward the lower ranges in OA and RA SF. We noted distinct alterations between cohorts in the relative distribution of PLs and the degree of FA saturation and chain lengths of FAs. CONCLUSIONS: The levels, composition, and MW distribution of all currently known lubricants in SF—HA, lubricin, PLs—vary with joint disease and stage of OA. Our study is the first delivering a comprehensive view about all joint lubricants during health and widespread joint diseases. Thus, we provide the framework to develop new optimal compounded lubricants to reduce joint destruction. Public Library of Science 2015-05-01 /pmc/articles/PMC4416892/ /pubmed/25933137 http://dx.doi.org/10.1371/journal.pone.0125192 Text en © 2015 Kosinska et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kosinska, Marta Krystyna
Ludwig, Taryn E.
Liebisch, Gerhard
Zhang, Ruiyan
Siebert, Hans-Christian
Wilhelm, Jochen
Kaesser, Ulrich
Dettmeyer, Reinhard B.
Klein, Heiko
Ishaque, Bernd
Rickert, Markus
Schmitz, Gerd
Schmidt, Tannin A.
Steinmeyer, Juergen
Articular Joint Lubricants during Osteoarthritis and Rheumatoid Arthritis Display Altered Levels and Molecular Species
title Articular Joint Lubricants during Osteoarthritis and Rheumatoid Arthritis Display Altered Levels and Molecular Species
title_full Articular Joint Lubricants during Osteoarthritis and Rheumatoid Arthritis Display Altered Levels and Molecular Species
title_fullStr Articular Joint Lubricants during Osteoarthritis and Rheumatoid Arthritis Display Altered Levels and Molecular Species
title_full_unstemmed Articular Joint Lubricants during Osteoarthritis and Rheumatoid Arthritis Display Altered Levels and Molecular Species
title_short Articular Joint Lubricants during Osteoarthritis and Rheumatoid Arthritis Display Altered Levels and Molecular Species
title_sort articular joint lubricants during osteoarthritis and rheumatoid arthritis display altered levels and molecular species
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4416892/
https://www.ncbi.nlm.nih.gov/pubmed/25933137
http://dx.doi.org/10.1371/journal.pone.0125192
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