Characterizing and controlling intrinsic biases of lambda exonuclease in nascent strand sequencing reveals phasing between nucleosomes and G-quadruplex motifs around a subset of human replication origins

Nascent strand sequencing (NS-seq) is used to discover DNA replication origins genome-wide, allowing identification of features for their specification. NS-seq depends on the ability of lambda exonuclease (λ-exo) to efficiently digest parental DNA while leaving RNA-primer protected nascent strands i...

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Detalles Bibliográficos
Autores principales: Foulk, Michael S., Urban, John M., Casella, Cinzia, Gerbi, Susan A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2015
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4417120/
https://www.ncbi.nlm.nih.gov/pubmed/25695952
http://dx.doi.org/10.1101/gr.183848.114

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4417120/
https://www.ncbi.nlm.nih.gov/pubmed/25695952
http://dx.doi.org/10.1101/gr.183848.114

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