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The nucleoporin Mlp2 is involved in chromosomal distribution during mitosis in trypanosomatids
Nucleoporins are evolutionary conserved proteins mainly involved in the constitution of the nuclear pores and trafficking between the nucleus and cytoplasm, but are also increasingly viewed as main actors in chromatin dynamics and intra-nuclear mitotic events. Here, we determined the cellular locali...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4417144/ https://www.ncbi.nlm.nih.gov/pubmed/25690889 http://dx.doi.org/10.1093/nar/gkv056 |
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author | Morelle, Christelle Sterkers, Yvon Crobu, Lucien MBang-Benet, Diane-Ethna Kuk, Nada Portalès, Pierre Bastien, Patrick Pagès, Michel Lachaud, Laurence |
author_facet | Morelle, Christelle Sterkers, Yvon Crobu, Lucien MBang-Benet, Diane-Ethna Kuk, Nada Portalès, Pierre Bastien, Patrick Pagès, Michel Lachaud, Laurence |
author_sort | Morelle, Christelle |
collection | PubMed |
description | Nucleoporins are evolutionary conserved proteins mainly involved in the constitution of the nuclear pores and trafficking between the nucleus and cytoplasm, but are also increasingly viewed as main actors in chromatin dynamics and intra-nuclear mitotic events. Here, we determined the cellular localization of the nucleoporin Mlp2 in the ‘divergent’ eukaryotes Leishmania major and Trypanosoma brucei. In both protozoa, Mlp2 displayed an atypical localization for a nucleoporin, essentially intranuclear, and preferentially in the periphery of the nucleolus during interphase; moreover, it relocated at the mitotic spindle poles during mitosis. In T. brucei, where most centromeres have been identified, TbMlp2 was found adjacent to the centromeric sequences, as well as to a recently described unconventional kinetochore protein, in the periphery of the nucleolus, during interphase and from the end of anaphase onwards. TbMlp2 and the centromeres/kinetochores exhibited a differential migration towards the poles during mitosis. RNAi knockdown of TbMlp2 disrupted the mitotic distribution of chromosomes, leading to a surprisingly well-tolerated aneuploidy. In addition, diploidy was restored in a complementation assay where LmMlp2, the orthologue of TbMlp2 in Leishmania, was expressed in TbMlp2-RNAi-knockdown parasites. Taken together, our results demonstrate that Mlp2 is involved in the distribution of chromosomes during mitosis in trypanosomatids. |
format | Online Article Text |
id | pubmed-4417144 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-44171442015-05-12 The nucleoporin Mlp2 is involved in chromosomal distribution during mitosis in trypanosomatids Morelle, Christelle Sterkers, Yvon Crobu, Lucien MBang-Benet, Diane-Ethna Kuk, Nada Portalès, Pierre Bastien, Patrick Pagès, Michel Lachaud, Laurence Nucleic Acids Res Genome Integrity, Repair and Replication Nucleoporins are evolutionary conserved proteins mainly involved in the constitution of the nuclear pores and trafficking between the nucleus and cytoplasm, but are also increasingly viewed as main actors in chromatin dynamics and intra-nuclear mitotic events. Here, we determined the cellular localization of the nucleoporin Mlp2 in the ‘divergent’ eukaryotes Leishmania major and Trypanosoma brucei. In both protozoa, Mlp2 displayed an atypical localization for a nucleoporin, essentially intranuclear, and preferentially in the periphery of the nucleolus during interphase; moreover, it relocated at the mitotic spindle poles during mitosis. In T. brucei, where most centromeres have been identified, TbMlp2 was found adjacent to the centromeric sequences, as well as to a recently described unconventional kinetochore protein, in the periphery of the nucleolus, during interphase and from the end of anaphase onwards. TbMlp2 and the centromeres/kinetochores exhibited a differential migration towards the poles during mitosis. RNAi knockdown of TbMlp2 disrupted the mitotic distribution of chromosomes, leading to a surprisingly well-tolerated aneuploidy. In addition, diploidy was restored in a complementation assay where LmMlp2, the orthologue of TbMlp2 in Leishmania, was expressed in TbMlp2-RNAi-knockdown parasites. Taken together, our results demonstrate that Mlp2 is involved in the distribution of chromosomes during mitosis in trypanosomatids. Oxford University Press 2015-04-30 2015-02-17 /pmc/articles/PMC4417144/ /pubmed/25690889 http://dx.doi.org/10.1093/nar/gkv056 Text en © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Genome Integrity, Repair and Replication Morelle, Christelle Sterkers, Yvon Crobu, Lucien MBang-Benet, Diane-Ethna Kuk, Nada Portalès, Pierre Bastien, Patrick Pagès, Michel Lachaud, Laurence The nucleoporin Mlp2 is involved in chromosomal distribution during mitosis in trypanosomatids |
title | The nucleoporin Mlp2 is involved in chromosomal distribution during mitosis in trypanosomatids |
title_full | The nucleoporin Mlp2 is involved in chromosomal distribution during mitosis in trypanosomatids |
title_fullStr | The nucleoporin Mlp2 is involved in chromosomal distribution during mitosis in trypanosomatids |
title_full_unstemmed | The nucleoporin Mlp2 is involved in chromosomal distribution during mitosis in trypanosomatids |
title_short | The nucleoporin Mlp2 is involved in chromosomal distribution during mitosis in trypanosomatids |
title_sort | nucleoporin mlp2 is involved in chromosomal distribution during mitosis in trypanosomatids |
topic | Genome Integrity, Repair and Replication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4417144/ https://www.ncbi.nlm.nih.gov/pubmed/25690889 http://dx.doi.org/10.1093/nar/gkv056 |
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