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The nucleoporin Mlp2 is involved in chromosomal distribution during mitosis in trypanosomatids

Nucleoporins are evolutionary conserved proteins mainly involved in the constitution of the nuclear pores and trafficking between the nucleus and cytoplasm, but are also increasingly viewed as main actors in chromatin dynamics and intra-nuclear mitotic events. Here, we determined the cellular locali...

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Autores principales: Morelle, Christelle, Sterkers, Yvon, Crobu, Lucien, MBang-Benet, Diane-Ethna, Kuk, Nada, Portalès, Pierre, Bastien, Patrick, Pagès, Michel, Lachaud, Laurence
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4417144/
https://www.ncbi.nlm.nih.gov/pubmed/25690889
http://dx.doi.org/10.1093/nar/gkv056
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author Morelle, Christelle
Sterkers, Yvon
Crobu, Lucien
MBang-Benet, Diane-Ethna
Kuk, Nada
Portalès, Pierre
Bastien, Patrick
Pagès, Michel
Lachaud, Laurence
author_facet Morelle, Christelle
Sterkers, Yvon
Crobu, Lucien
MBang-Benet, Diane-Ethna
Kuk, Nada
Portalès, Pierre
Bastien, Patrick
Pagès, Michel
Lachaud, Laurence
author_sort Morelle, Christelle
collection PubMed
description Nucleoporins are evolutionary conserved proteins mainly involved in the constitution of the nuclear pores and trafficking between the nucleus and cytoplasm, but are also increasingly viewed as main actors in chromatin dynamics and intra-nuclear mitotic events. Here, we determined the cellular localization of the nucleoporin Mlp2 in the ‘divergent’ eukaryotes Leishmania major and Trypanosoma brucei. In both protozoa, Mlp2 displayed an atypical localization for a nucleoporin, essentially intranuclear, and preferentially in the periphery of the nucleolus during interphase; moreover, it relocated at the mitotic spindle poles during mitosis. In T. brucei, where most centromeres have been identified, TbMlp2 was found adjacent to the centromeric sequences, as well as to a recently described unconventional kinetochore protein, in the periphery of the nucleolus, during interphase and from the end of anaphase onwards. TbMlp2 and the centromeres/kinetochores exhibited a differential migration towards the poles during mitosis. RNAi knockdown of TbMlp2 disrupted the mitotic distribution of chromosomes, leading to a surprisingly well-tolerated aneuploidy. In addition, diploidy was restored in a complementation assay where LmMlp2, the orthologue of TbMlp2 in Leishmania, was expressed in TbMlp2-RNAi-knockdown parasites. Taken together, our results demonstrate that Mlp2 is involved in the distribution of chromosomes during mitosis in trypanosomatids.
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spelling pubmed-44171442015-05-12 The nucleoporin Mlp2 is involved in chromosomal distribution during mitosis in trypanosomatids Morelle, Christelle Sterkers, Yvon Crobu, Lucien MBang-Benet, Diane-Ethna Kuk, Nada Portalès, Pierre Bastien, Patrick Pagès, Michel Lachaud, Laurence Nucleic Acids Res Genome Integrity, Repair and Replication Nucleoporins are evolutionary conserved proteins mainly involved in the constitution of the nuclear pores and trafficking between the nucleus and cytoplasm, but are also increasingly viewed as main actors in chromatin dynamics and intra-nuclear mitotic events. Here, we determined the cellular localization of the nucleoporin Mlp2 in the ‘divergent’ eukaryotes Leishmania major and Trypanosoma brucei. In both protozoa, Mlp2 displayed an atypical localization for a nucleoporin, essentially intranuclear, and preferentially in the periphery of the nucleolus during interphase; moreover, it relocated at the mitotic spindle poles during mitosis. In T. brucei, where most centromeres have been identified, TbMlp2 was found adjacent to the centromeric sequences, as well as to a recently described unconventional kinetochore protein, in the periphery of the nucleolus, during interphase and from the end of anaphase onwards. TbMlp2 and the centromeres/kinetochores exhibited a differential migration towards the poles during mitosis. RNAi knockdown of TbMlp2 disrupted the mitotic distribution of chromosomes, leading to a surprisingly well-tolerated aneuploidy. In addition, diploidy was restored in a complementation assay where LmMlp2, the orthologue of TbMlp2 in Leishmania, was expressed in TbMlp2-RNAi-knockdown parasites. Taken together, our results demonstrate that Mlp2 is involved in the distribution of chromosomes during mitosis in trypanosomatids. Oxford University Press 2015-04-30 2015-02-17 /pmc/articles/PMC4417144/ /pubmed/25690889 http://dx.doi.org/10.1093/nar/gkv056 Text en © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Genome Integrity, Repair and Replication
Morelle, Christelle
Sterkers, Yvon
Crobu, Lucien
MBang-Benet, Diane-Ethna
Kuk, Nada
Portalès, Pierre
Bastien, Patrick
Pagès, Michel
Lachaud, Laurence
The nucleoporin Mlp2 is involved in chromosomal distribution during mitosis in trypanosomatids
title The nucleoporin Mlp2 is involved in chromosomal distribution during mitosis in trypanosomatids
title_full The nucleoporin Mlp2 is involved in chromosomal distribution during mitosis in trypanosomatids
title_fullStr The nucleoporin Mlp2 is involved in chromosomal distribution during mitosis in trypanosomatids
title_full_unstemmed The nucleoporin Mlp2 is involved in chromosomal distribution during mitosis in trypanosomatids
title_short The nucleoporin Mlp2 is involved in chromosomal distribution during mitosis in trypanosomatids
title_sort nucleoporin mlp2 is involved in chromosomal distribution during mitosis in trypanosomatids
topic Genome Integrity, Repair and Replication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4417144/
https://www.ncbi.nlm.nih.gov/pubmed/25690889
http://dx.doi.org/10.1093/nar/gkv056
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