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Selective inhibition of miR-21 by phage display screened peptide

miRNAs are nodal regulators of gene expression and deregulation of miRNAs is causally associated with different diseases, including cancer. Modulation of miRNA expression is thus of therapeutic importance. Small molecules are currently being explored for their potential to downregulate miRNAs. Pepti...

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Detalles Bibliográficos
Autores principales: Bose, Debojit, Nahar, Smita, Rai, Manish Kumar, Ray, Arjun, Chakraborty, Kausik, Maiti, Souvik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4417150/
https://www.ncbi.nlm.nih.gov/pubmed/25824952
http://dx.doi.org/10.1093/nar/gkv185
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author Bose, Debojit
Nahar, Smita
Rai, Manish Kumar
Ray, Arjun
Chakraborty, Kausik
Maiti, Souvik
author_facet Bose, Debojit
Nahar, Smita
Rai, Manish Kumar
Ray, Arjun
Chakraborty, Kausik
Maiti, Souvik
author_sort Bose, Debojit
collection PubMed
description miRNAs are nodal regulators of gene expression and deregulation of miRNAs is causally associated with different diseases, including cancer. Modulation of miRNA expression is thus of therapeutic importance. Small molecules are currently being explored for their potential to downregulate miRNAs. Peptides have shown to have better potency and selectivity toward their targets but their potential in targeting and modulating miRNAs remain unexplored. Herein, using phage display we found a very selective peptide against pre-miR-21. Interestingly, the peptide has the potential to downregulate miR-21, by binding to pre-miR-21 and hindering Dicer processing. It is selective towards miR-21 inside the cell. By antagonising miR-21 function, the peptide is able to increase the expression of its target proteins and thereby increase apoptosis and suppress cell proliferation, invasion and migration. This peptide can further be explored for its anti-cancer activity in vivo and may be even extended to clinical studies.
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spelling pubmed-44171502015-05-12 Selective inhibition of miR-21 by phage display screened peptide Bose, Debojit Nahar, Smita Rai, Manish Kumar Ray, Arjun Chakraborty, Kausik Maiti, Souvik Nucleic Acids Res Synthetic Biology and Bioengineering miRNAs are nodal regulators of gene expression and deregulation of miRNAs is causally associated with different diseases, including cancer. Modulation of miRNA expression is thus of therapeutic importance. Small molecules are currently being explored for their potential to downregulate miRNAs. Peptides have shown to have better potency and selectivity toward their targets but their potential in targeting and modulating miRNAs remain unexplored. Herein, using phage display we found a very selective peptide against pre-miR-21. Interestingly, the peptide has the potential to downregulate miR-21, by binding to pre-miR-21 and hindering Dicer processing. It is selective towards miR-21 inside the cell. By antagonising miR-21 function, the peptide is able to increase the expression of its target proteins and thereby increase apoptosis and suppress cell proliferation, invasion and migration. This peptide can further be explored for its anti-cancer activity in vivo and may be even extended to clinical studies. Oxford University Press 2015-04-30 2015-03-30 /pmc/articles/PMC4417150/ /pubmed/25824952 http://dx.doi.org/10.1093/nar/gkv185 Text en © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Synthetic Biology and Bioengineering
Bose, Debojit
Nahar, Smita
Rai, Manish Kumar
Ray, Arjun
Chakraborty, Kausik
Maiti, Souvik
Selective inhibition of miR-21 by phage display screened peptide
title Selective inhibition of miR-21 by phage display screened peptide
title_full Selective inhibition of miR-21 by phage display screened peptide
title_fullStr Selective inhibition of miR-21 by phage display screened peptide
title_full_unstemmed Selective inhibition of miR-21 by phage display screened peptide
title_short Selective inhibition of miR-21 by phage display screened peptide
title_sort selective inhibition of mir-21 by phage display screened peptide
topic Synthetic Biology and Bioengineering
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4417150/
https://www.ncbi.nlm.nih.gov/pubmed/25824952
http://dx.doi.org/10.1093/nar/gkv185
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