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Subunit compositions of Arabidopsis RNA polymerases I and III reveal Pol I- and Pol III-specific forms of the AC40 subunit and alternative forms of the C53 subunit

Using affinity purification and mass spectrometry, we identified the subunits of Arabidopsis thaliana multisubunit RNA polymerases I and III (abbreviated as Pol I and Pol III), the first analysis of their physical compositions in plants. In all eukaryotes examined to date, AC40 and AC19 subunits are...

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Autores principales: Ream, Thomas S., Haag, Jeremy R., Pontvianne, Frederic, Nicora, Carrie D., Norbeck, Angela D., Paša-Tolić, Ljiljana, Pikaard, Craig S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4417161/
https://www.ncbi.nlm.nih.gov/pubmed/25813043
http://dx.doi.org/10.1093/nar/gkv247
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author Ream, Thomas S.
Haag, Jeremy R.
Pontvianne, Frederic
Nicora, Carrie D.
Norbeck, Angela D.
Paša-Tolić, Ljiljana
Pikaard, Craig S.
author_facet Ream, Thomas S.
Haag, Jeremy R.
Pontvianne, Frederic
Nicora, Carrie D.
Norbeck, Angela D.
Paša-Tolić, Ljiljana
Pikaard, Craig S.
author_sort Ream, Thomas S.
collection PubMed
description Using affinity purification and mass spectrometry, we identified the subunits of Arabidopsis thaliana multisubunit RNA polymerases I and III (abbreviated as Pol I and Pol III), the first analysis of their physical compositions in plants. In all eukaryotes examined to date, AC40 and AC19 subunits are common to Pol I (a.k.a. Pol A) and Pol III (a.k.a. Pol C) and are encoded by single genes. Surprisingly, A. thaliana and related species express two distinct AC40 paralogs, one of which assembles into Pol I and the other of which assembles into Pol III. Changes at eight amino acid positions correlate with the functional divergence of Pol I- and Pol III-specific AC40 paralogs. Two genes encode homologs of the yeast C53 subunit and either protein can assemble into Pol III. By contrast, only one of two potential C17 variants, and one of two potential C31 variants were detected in Pol III. We introduce a new nomenclature system for plant Pol I and Pol III subunits in which the 12 subunits that are structurally and functionally homologous among Pols I through V are assigned equivalent numbers.
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spelling pubmed-44171612015-05-12 Subunit compositions of Arabidopsis RNA polymerases I and III reveal Pol I- and Pol III-specific forms of the AC40 subunit and alternative forms of the C53 subunit Ream, Thomas S. Haag, Jeremy R. Pontvianne, Frederic Nicora, Carrie D. Norbeck, Angela D. Paša-Tolić, Ljiljana Pikaard, Craig S. Nucleic Acids Res Nucleic Acid Enzymes Using affinity purification and mass spectrometry, we identified the subunits of Arabidopsis thaliana multisubunit RNA polymerases I and III (abbreviated as Pol I and Pol III), the first analysis of their physical compositions in plants. In all eukaryotes examined to date, AC40 and AC19 subunits are common to Pol I (a.k.a. Pol A) and Pol III (a.k.a. Pol C) and are encoded by single genes. Surprisingly, A. thaliana and related species express two distinct AC40 paralogs, one of which assembles into Pol I and the other of which assembles into Pol III. Changes at eight amino acid positions correlate with the functional divergence of Pol I- and Pol III-specific AC40 paralogs. Two genes encode homologs of the yeast C53 subunit and either protein can assemble into Pol III. By contrast, only one of two potential C17 variants, and one of two potential C31 variants were detected in Pol III. We introduce a new nomenclature system for plant Pol I and Pol III subunits in which the 12 subunits that are structurally and functionally homologous among Pols I through V are assigned equivalent numbers. Oxford University Press 2015-04-30 2015-03-26 /pmc/articles/PMC4417161/ /pubmed/25813043 http://dx.doi.org/10.1093/nar/gkv247 Text en © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Nucleic Acid Enzymes
Ream, Thomas S.
Haag, Jeremy R.
Pontvianne, Frederic
Nicora, Carrie D.
Norbeck, Angela D.
Paša-Tolić, Ljiljana
Pikaard, Craig S.
Subunit compositions of Arabidopsis RNA polymerases I and III reveal Pol I- and Pol III-specific forms of the AC40 subunit and alternative forms of the C53 subunit
title Subunit compositions of Arabidopsis RNA polymerases I and III reveal Pol I- and Pol III-specific forms of the AC40 subunit and alternative forms of the C53 subunit
title_full Subunit compositions of Arabidopsis RNA polymerases I and III reveal Pol I- and Pol III-specific forms of the AC40 subunit and alternative forms of the C53 subunit
title_fullStr Subunit compositions of Arabidopsis RNA polymerases I and III reveal Pol I- and Pol III-specific forms of the AC40 subunit and alternative forms of the C53 subunit
title_full_unstemmed Subunit compositions of Arabidopsis RNA polymerases I and III reveal Pol I- and Pol III-specific forms of the AC40 subunit and alternative forms of the C53 subunit
title_short Subunit compositions of Arabidopsis RNA polymerases I and III reveal Pol I- and Pol III-specific forms of the AC40 subunit and alternative forms of the C53 subunit
title_sort subunit compositions of arabidopsis rna polymerases i and iii reveal pol i- and pol iii-specific forms of the ac40 subunit and alternative forms of the c53 subunit
topic Nucleic Acid Enzymes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4417161/
https://www.ncbi.nlm.nih.gov/pubmed/25813043
http://dx.doi.org/10.1093/nar/gkv247
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