Cargando…

A microRNA profile associated with Opisthorchis viverrini-induced cholangiocarcinoma in tissue and plasma

BACKGROUND: Intrahepatic cholangiocarcinoma (ICC) is a highly aggressive tumor of the bile duct, and a significant public health problem in East Asia, where it is associated with infection by the parasite Opisthorchis viverrini. ICC is often detected at an advanced stage and with a poor prognosis, m...

Descripción completa

Detalles Bibliográficos
Autores principales: Plieskatt, Jordan, Rinaldi, Gabriel, Feng, Yanjun, Peng, Jin, Easley, Samantha, Jia, Xinying, Potriquet, Jeremy, Pairojkul, Chawalit, Bhudhisawasdi, Vajarabhongsa, Sripa, Banchob, Brindley, Paul J, Bethony, Jeffrey, Mulvenna, Jason
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4417245/
https://www.ncbi.nlm.nih.gov/pubmed/25903557
http://dx.doi.org/10.1186/s12885-015-1270-5
_version_ 1782369339399208960
author Plieskatt, Jordan
Rinaldi, Gabriel
Feng, Yanjun
Peng, Jin
Easley, Samantha
Jia, Xinying
Potriquet, Jeremy
Pairojkul, Chawalit
Bhudhisawasdi, Vajarabhongsa
Sripa, Banchob
Brindley, Paul J
Bethony, Jeffrey
Mulvenna, Jason
author_facet Plieskatt, Jordan
Rinaldi, Gabriel
Feng, Yanjun
Peng, Jin
Easley, Samantha
Jia, Xinying
Potriquet, Jeremy
Pairojkul, Chawalit
Bhudhisawasdi, Vajarabhongsa
Sripa, Banchob
Brindley, Paul J
Bethony, Jeffrey
Mulvenna, Jason
author_sort Plieskatt, Jordan
collection PubMed
description BACKGROUND: Intrahepatic cholangiocarcinoma (ICC) is a highly aggressive tumor of the bile duct, and a significant public health problem in East Asia, where it is associated with infection by the parasite Opisthorchis viverrini. ICC is often detected at an advanced stage and with a poor prognosis, making a biomarker for early detection a priority. METHODS: We have comprehensively profiled miRNA expression levels in ICC tumor tissue using small RNA-Seq and validated these profiles using quantitative PCR on matched plasma samples. RESULTS: Distinct miRNA profiles were associated with increasing histological differentiation of ICC tumor tissue. We also observed that histologically normal tissue adjacent to ICC tumor displayed miRNA expression profiles more similar to tumor than liver tissue from healthy donors. In plasma samples, an eight-miRNA signature associated with ICC, regardless of the degree of histological differentiation of its matched tissue, forming the basis of a circulating miRNA-based biomarker for ICC. CONCLUSIONS: The association of unique miRNA profiles with different ICC subtypes suggests the involvement of specific miRNAs during ICC tumor progression. In plasma, an eight-miRNA signature associated with ICC could form the foundation of an accessible (plasma-based) miRNA-based biomarker for the early detection of ICC. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12885-015-1270-5) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-4417245
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-44172452015-05-03 A microRNA profile associated with Opisthorchis viverrini-induced cholangiocarcinoma in tissue and plasma Plieskatt, Jordan Rinaldi, Gabriel Feng, Yanjun Peng, Jin Easley, Samantha Jia, Xinying Potriquet, Jeremy Pairojkul, Chawalit Bhudhisawasdi, Vajarabhongsa Sripa, Banchob Brindley, Paul J Bethony, Jeffrey Mulvenna, Jason BMC Cancer Research Article BACKGROUND: Intrahepatic cholangiocarcinoma (ICC) is a highly aggressive tumor of the bile duct, and a significant public health problem in East Asia, where it is associated with infection by the parasite Opisthorchis viverrini. ICC is often detected at an advanced stage and with a poor prognosis, making a biomarker for early detection a priority. METHODS: We have comprehensively profiled miRNA expression levels in ICC tumor tissue using small RNA-Seq and validated these profiles using quantitative PCR on matched plasma samples. RESULTS: Distinct miRNA profiles were associated with increasing histological differentiation of ICC tumor tissue. We also observed that histologically normal tissue adjacent to ICC tumor displayed miRNA expression profiles more similar to tumor than liver tissue from healthy donors. In plasma samples, an eight-miRNA signature associated with ICC, regardless of the degree of histological differentiation of its matched tissue, forming the basis of a circulating miRNA-based biomarker for ICC. CONCLUSIONS: The association of unique miRNA profiles with different ICC subtypes suggests the involvement of specific miRNAs during ICC tumor progression. In plasma, an eight-miRNA signature associated with ICC could form the foundation of an accessible (plasma-based) miRNA-based biomarker for the early detection of ICC. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12885-015-1270-5) contains supplementary material, which is available to authorized users. BioMed Central 2015-04-23 /pmc/articles/PMC4417245/ /pubmed/25903557 http://dx.doi.org/10.1186/s12885-015-1270-5 Text en © Plieskatt et al.; licensee BioMed Central. 2015 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Plieskatt, Jordan
Rinaldi, Gabriel
Feng, Yanjun
Peng, Jin
Easley, Samantha
Jia, Xinying
Potriquet, Jeremy
Pairojkul, Chawalit
Bhudhisawasdi, Vajarabhongsa
Sripa, Banchob
Brindley, Paul J
Bethony, Jeffrey
Mulvenna, Jason
A microRNA profile associated with Opisthorchis viverrini-induced cholangiocarcinoma in tissue and plasma
title A microRNA profile associated with Opisthorchis viverrini-induced cholangiocarcinoma in tissue and plasma
title_full A microRNA profile associated with Opisthorchis viverrini-induced cholangiocarcinoma in tissue and plasma
title_fullStr A microRNA profile associated with Opisthorchis viverrini-induced cholangiocarcinoma in tissue and plasma
title_full_unstemmed A microRNA profile associated with Opisthorchis viverrini-induced cholangiocarcinoma in tissue and plasma
title_short A microRNA profile associated with Opisthorchis viverrini-induced cholangiocarcinoma in tissue and plasma
title_sort microrna profile associated with opisthorchis viverrini-induced cholangiocarcinoma in tissue and plasma
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4417245/
https://www.ncbi.nlm.nih.gov/pubmed/25903557
http://dx.doi.org/10.1186/s12885-015-1270-5
work_keys_str_mv AT plieskattjordan amicrornaprofileassociatedwithopisthorchisviverriniinducedcholangiocarcinomaintissueandplasma
AT rinaldigabriel amicrornaprofileassociatedwithopisthorchisviverriniinducedcholangiocarcinomaintissueandplasma
AT fengyanjun amicrornaprofileassociatedwithopisthorchisviverriniinducedcholangiocarcinomaintissueandplasma
AT pengjin amicrornaprofileassociatedwithopisthorchisviverriniinducedcholangiocarcinomaintissueandplasma
AT easleysamantha amicrornaprofileassociatedwithopisthorchisviverriniinducedcholangiocarcinomaintissueandplasma
AT jiaxinying amicrornaprofileassociatedwithopisthorchisviverriniinducedcholangiocarcinomaintissueandplasma
AT potriquetjeremy amicrornaprofileassociatedwithopisthorchisviverriniinducedcholangiocarcinomaintissueandplasma
AT pairojkulchawalit amicrornaprofileassociatedwithopisthorchisviverriniinducedcholangiocarcinomaintissueandplasma
AT bhudhisawasdivajarabhongsa amicrornaprofileassociatedwithopisthorchisviverriniinducedcholangiocarcinomaintissueandplasma
AT sripabanchob amicrornaprofileassociatedwithopisthorchisviverriniinducedcholangiocarcinomaintissueandplasma
AT brindleypaulj amicrornaprofileassociatedwithopisthorchisviverriniinducedcholangiocarcinomaintissueandplasma
AT bethonyjeffrey amicrornaprofileassociatedwithopisthorchisviverriniinducedcholangiocarcinomaintissueandplasma
AT mulvennajason amicrornaprofileassociatedwithopisthorchisviverriniinducedcholangiocarcinomaintissueandplasma
AT plieskattjordan micrornaprofileassociatedwithopisthorchisviverriniinducedcholangiocarcinomaintissueandplasma
AT rinaldigabriel micrornaprofileassociatedwithopisthorchisviverriniinducedcholangiocarcinomaintissueandplasma
AT fengyanjun micrornaprofileassociatedwithopisthorchisviverriniinducedcholangiocarcinomaintissueandplasma
AT pengjin micrornaprofileassociatedwithopisthorchisviverriniinducedcholangiocarcinomaintissueandplasma
AT easleysamantha micrornaprofileassociatedwithopisthorchisviverriniinducedcholangiocarcinomaintissueandplasma
AT jiaxinying micrornaprofileassociatedwithopisthorchisviverriniinducedcholangiocarcinomaintissueandplasma
AT potriquetjeremy micrornaprofileassociatedwithopisthorchisviverriniinducedcholangiocarcinomaintissueandplasma
AT pairojkulchawalit micrornaprofileassociatedwithopisthorchisviverriniinducedcholangiocarcinomaintissueandplasma
AT bhudhisawasdivajarabhongsa micrornaprofileassociatedwithopisthorchisviverriniinducedcholangiocarcinomaintissueandplasma
AT sripabanchob micrornaprofileassociatedwithopisthorchisviverriniinducedcholangiocarcinomaintissueandplasma
AT brindleypaulj micrornaprofileassociatedwithopisthorchisviverriniinducedcholangiocarcinomaintissueandplasma
AT bethonyjeffrey micrornaprofileassociatedwithopisthorchisviverriniinducedcholangiocarcinomaintissueandplasma
AT mulvennajason micrornaprofileassociatedwithopisthorchisviverriniinducedcholangiocarcinomaintissueandplasma