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Plasma Lactate Levels Increase during Hyperinsulinemic Euglycemic Clamp and Oral Glucose Tolerance Test

Insulin resistance, which plays a central role in the pathogenesis of type 2 diabetes (T2D), is an early indicator that heralds the occurrence of T2D. It is imperative to understand the metabolic changes that occur at the cellular level in the early stages of insulin resistance. The objective of thi...

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Autores principales: Berhane, Feven, Fite, Alemu, Daboul, Nour, Al-Janabi, Wissam, Msallaty, Zaher, Caruso, Michael, Lewis, Monique K., Yi, Zhengping, Diamond, Michael P., Abou-Samra, Abdul-Badi, Seyoum, Berhane
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4417566/
https://www.ncbi.nlm.nih.gov/pubmed/25961050
http://dx.doi.org/10.1155/2015/102054
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author Berhane, Feven
Fite, Alemu
Daboul, Nour
Al-Janabi, Wissam
Msallaty, Zaher
Caruso, Michael
Lewis, Monique K.
Yi, Zhengping
Diamond, Michael P.
Abou-Samra, Abdul-Badi
Seyoum, Berhane
author_facet Berhane, Feven
Fite, Alemu
Daboul, Nour
Al-Janabi, Wissam
Msallaty, Zaher
Caruso, Michael
Lewis, Monique K.
Yi, Zhengping
Diamond, Michael P.
Abou-Samra, Abdul-Badi
Seyoum, Berhane
author_sort Berhane, Feven
collection PubMed
description Insulin resistance, which plays a central role in the pathogenesis of type 2 diabetes (T2D), is an early indicator that heralds the occurrence of T2D. It is imperative to understand the metabolic changes that occur at the cellular level in the early stages of insulin resistance. The objective of this study was to determine the pattern of circulating lactate levels during oral glucose tolerance test (OGTT) and hyperinsulinemic euglycemic clamp (HIEC) study in normal nondiabetic subjects. Lactate and glycerol were determined every 30 minutes during OGTT and HIEC on 22 participants. Lactate progressively increased throughout the HIEC study period (P < 0.001). Participants with BMI < 30 had significantly higher mean M-values compared to those with BMI ≥ 30 at baseline (P < 0.05). This trend also continued throughout the OGTT. In addition, those with impaired glucose tolerance test (IGT) had significantly higher mean lactate levels compared to those with normal glucose tolerance (P < 0.001). In conclusion, we found that lactate increased during HIEC study, which is a state of hyperinsulinemia similar to the metabolic milieu seen during the early stages in the development of T2D.
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spelling pubmed-44175662015-05-10 Plasma Lactate Levels Increase during Hyperinsulinemic Euglycemic Clamp and Oral Glucose Tolerance Test Berhane, Feven Fite, Alemu Daboul, Nour Al-Janabi, Wissam Msallaty, Zaher Caruso, Michael Lewis, Monique K. Yi, Zhengping Diamond, Michael P. Abou-Samra, Abdul-Badi Seyoum, Berhane J Diabetes Res Research Article Insulin resistance, which plays a central role in the pathogenesis of type 2 diabetes (T2D), is an early indicator that heralds the occurrence of T2D. It is imperative to understand the metabolic changes that occur at the cellular level in the early stages of insulin resistance. The objective of this study was to determine the pattern of circulating lactate levels during oral glucose tolerance test (OGTT) and hyperinsulinemic euglycemic clamp (HIEC) study in normal nondiabetic subjects. Lactate and glycerol were determined every 30 minutes during OGTT and HIEC on 22 participants. Lactate progressively increased throughout the HIEC study period (P < 0.001). Participants with BMI < 30 had significantly higher mean M-values compared to those with BMI ≥ 30 at baseline (P < 0.05). This trend also continued throughout the OGTT. In addition, those with impaired glucose tolerance test (IGT) had significantly higher mean lactate levels compared to those with normal glucose tolerance (P < 0.001). In conclusion, we found that lactate increased during HIEC study, which is a state of hyperinsulinemia similar to the metabolic milieu seen during the early stages in the development of T2D. Hindawi Publishing Corporation 2015 2015-04-19 /pmc/articles/PMC4417566/ /pubmed/25961050 http://dx.doi.org/10.1155/2015/102054 Text en Copyright © 2015 Feven Berhane et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Berhane, Feven
Fite, Alemu
Daboul, Nour
Al-Janabi, Wissam
Msallaty, Zaher
Caruso, Michael
Lewis, Monique K.
Yi, Zhengping
Diamond, Michael P.
Abou-Samra, Abdul-Badi
Seyoum, Berhane
Plasma Lactate Levels Increase during Hyperinsulinemic Euglycemic Clamp and Oral Glucose Tolerance Test
title Plasma Lactate Levels Increase during Hyperinsulinemic Euglycemic Clamp and Oral Glucose Tolerance Test
title_full Plasma Lactate Levels Increase during Hyperinsulinemic Euglycemic Clamp and Oral Glucose Tolerance Test
title_fullStr Plasma Lactate Levels Increase during Hyperinsulinemic Euglycemic Clamp and Oral Glucose Tolerance Test
title_full_unstemmed Plasma Lactate Levels Increase during Hyperinsulinemic Euglycemic Clamp and Oral Glucose Tolerance Test
title_short Plasma Lactate Levels Increase during Hyperinsulinemic Euglycemic Clamp and Oral Glucose Tolerance Test
title_sort plasma lactate levels increase during hyperinsulinemic euglycemic clamp and oral glucose tolerance test
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4417566/
https://www.ncbi.nlm.nih.gov/pubmed/25961050
http://dx.doi.org/10.1155/2015/102054
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