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Application of FTIR-ATR Spectroscopy to Determine the Extent of Lipid Peroxidation in Plasma during Haemodialysis
During a haemodialysis (HD), because of the contact of blood with the surface of the dialyser, the immune system becomes activated and reactive oxygen species (ROS) are released into plasma. Particularly exposed to the ROS are lipids and proteins contained in plasma, which undergo peroxidation. The...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4417580/ https://www.ncbi.nlm.nih.gov/pubmed/25961007 http://dx.doi.org/10.1155/2015/245607 |
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author | Oleszko, Adam Olsztyńska-Janus, Sylwia Walski, Tomasz Grzeszczuk-Kuć, Karolina Bujok, Jolanta Gałecka, Katarzyna Czerski, Albert Witkiewicz, Wojciech Komorowska, Małgorzata |
author_facet | Oleszko, Adam Olsztyńska-Janus, Sylwia Walski, Tomasz Grzeszczuk-Kuć, Karolina Bujok, Jolanta Gałecka, Katarzyna Czerski, Albert Witkiewicz, Wojciech Komorowska, Małgorzata |
author_sort | Oleszko, Adam |
collection | PubMed |
description | During a haemodialysis (HD), because of the contact of blood with the surface of the dialyser, the immune system becomes activated and reactive oxygen species (ROS) are released into plasma. Particularly exposed to the ROS are lipids and proteins contained in plasma, which undergo peroxidation. The main breakdown product of oxidized lipids is the malondialdehyde (MDA). A common method for measuring the concentration of MDA is a thiobarbituric acid reactive substances (TBARS) method. Despite the formation of MDA in plasma during HD, its concentration decreases because it is removed from the blood in the dialyser. Therefore, this research proposes the Fourier Transform Infrared Attenuated Total Reflectance (FTIR-ATR) spectroscopy, which enables determination of primary peroxidation products. We examined the influence of the amount of hydrogen peroxide added to lipid suspension that was earlier extracted from plasma specimen on lipid peroxidation with use of TBARS and FTIR-ATR methods. Linear correlation between these methods was shown. The proposed method was effective during the evaluation of changes in the extent of lipid peroxidation in plasma during a haemodialysis in sheep. A measurement using the FTIR-ATR showed an increase in plasma lipid peroxidation after 15 and 240 minutes of treatment, while the TBARS concentration was respectively lower. |
format | Online Article Text |
id | pubmed-4417580 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-44175802015-05-10 Application of FTIR-ATR Spectroscopy to Determine the Extent of Lipid Peroxidation in Plasma during Haemodialysis Oleszko, Adam Olsztyńska-Janus, Sylwia Walski, Tomasz Grzeszczuk-Kuć, Karolina Bujok, Jolanta Gałecka, Katarzyna Czerski, Albert Witkiewicz, Wojciech Komorowska, Małgorzata Biomed Res Int Research Article During a haemodialysis (HD), because of the contact of blood with the surface of the dialyser, the immune system becomes activated and reactive oxygen species (ROS) are released into plasma. Particularly exposed to the ROS are lipids and proteins contained in plasma, which undergo peroxidation. The main breakdown product of oxidized lipids is the malondialdehyde (MDA). A common method for measuring the concentration of MDA is a thiobarbituric acid reactive substances (TBARS) method. Despite the formation of MDA in plasma during HD, its concentration decreases because it is removed from the blood in the dialyser. Therefore, this research proposes the Fourier Transform Infrared Attenuated Total Reflectance (FTIR-ATR) spectroscopy, which enables determination of primary peroxidation products. We examined the influence of the amount of hydrogen peroxide added to lipid suspension that was earlier extracted from plasma specimen on lipid peroxidation with use of TBARS and FTIR-ATR methods. Linear correlation between these methods was shown. The proposed method was effective during the evaluation of changes in the extent of lipid peroxidation in plasma during a haemodialysis in sheep. A measurement using the FTIR-ATR showed an increase in plasma lipid peroxidation after 15 and 240 minutes of treatment, while the TBARS concentration was respectively lower. Hindawi Publishing Corporation 2015 2015-04-19 /pmc/articles/PMC4417580/ /pubmed/25961007 http://dx.doi.org/10.1155/2015/245607 Text en Copyright © 2015 Adam Oleszko et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Oleszko, Adam Olsztyńska-Janus, Sylwia Walski, Tomasz Grzeszczuk-Kuć, Karolina Bujok, Jolanta Gałecka, Katarzyna Czerski, Albert Witkiewicz, Wojciech Komorowska, Małgorzata Application of FTIR-ATR Spectroscopy to Determine the Extent of Lipid Peroxidation in Plasma during Haemodialysis |
title | Application of FTIR-ATR Spectroscopy to Determine the Extent of Lipid Peroxidation in Plasma during Haemodialysis |
title_full | Application of FTIR-ATR Spectroscopy to Determine the Extent of Lipid Peroxidation in Plasma during Haemodialysis |
title_fullStr | Application of FTIR-ATR Spectroscopy to Determine the Extent of Lipid Peroxidation in Plasma during Haemodialysis |
title_full_unstemmed | Application of FTIR-ATR Spectroscopy to Determine the Extent of Lipid Peroxidation in Plasma during Haemodialysis |
title_short | Application of FTIR-ATR Spectroscopy to Determine the Extent of Lipid Peroxidation in Plasma during Haemodialysis |
title_sort | application of ftir-atr spectroscopy to determine the extent of lipid peroxidation in plasma during haemodialysis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4417580/ https://www.ncbi.nlm.nih.gov/pubmed/25961007 http://dx.doi.org/10.1155/2015/245607 |
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