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Detection of Shiga Toxins by Lateral Flow Assay
Shiga toxin-producing Escherichia coli (STEC) produce shiga toxins (Stxs) that can cause human disease and death. The contamination of food products with STEC represents a food safety problem that necessitates rapid and effective detection strategies to mitigate risk. In this manuscript, we report t...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4417961/ https://www.ncbi.nlm.nih.gov/pubmed/25855129 http://dx.doi.org/10.3390/toxins7041163 |
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author | Ching, Kathryn H. He, Xiaohua Stanker, Larry H. Lin, Alice V. McGarvey, Jeffery A. Hnasko, Robert |
author_facet | Ching, Kathryn H. He, Xiaohua Stanker, Larry H. Lin, Alice V. McGarvey, Jeffery A. Hnasko, Robert |
author_sort | Ching, Kathryn H. |
collection | PubMed |
description | Shiga toxin-producing Escherichia coli (STEC) produce shiga toxins (Stxs) that can cause human disease and death. The contamination of food products with STEC represents a food safety problem that necessitates rapid and effective detection strategies to mitigate risk. In this manuscript, we report the development of a colorimetric lateral flow assay (LFA) for the rapid detection of Stxs in <10 min using a pair of monoclonal antibodies that bind epitopes common to Stx1 and six Stx2 variants. This LFA provides a rapid and sensitive test for the detection of Stxs directly from STEC culture supernatants or at risk food samples with a 0.1 ng/mL limit of detection (LOD) for Stx2a. This Stx LFA is applicable for use in the rapid evaluation of Stx production from cultured E. coli strains or as a tool to augment current methods as part of food safety testing. |
format | Online Article Text |
id | pubmed-4417961 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-44179612015-05-18 Detection of Shiga Toxins by Lateral Flow Assay Ching, Kathryn H. He, Xiaohua Stanker, Larry H. Lin, Alice V. McGarvey, Jeffery A. Hnasko, Robert Toxins (Basel) Article Shiga toxin-producing Escherichia coli (STEC) produce shiga toxins (Stxs) that can cause human disease and death. The contamination of food products with STEC represents a food safety problem that necessitates rapid and effective detection strategies to mitigate risk. In this manuscript, we report the development of a colorimetric lateral flow assay (LFA) for the rapid detection of Stxs in <10 min using a pair of monoclonal antibodies that bind epitopes common to Stx1 and six Stx2 variants. This LFA provides a rapid and sensitive test for the detection of Stxs directly from STEC culture supernatants or at risk food samples with a 0.1 ng/mL limit of detection (LOD) for Stx2a. This Stx LFA is applicable for use in the rapid evaluation of Stx production from cultured E. coli strains or as a tool to augment current methods as part of food safety testing. MDPI 2015-04-03 /pmc/articles/PMC4417961/ /pubmed/25855129 http://dx.doi.org/10.3390/toxins7041163 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ching, Kathryn H. He, Xiaohua Stanker, Larry H. Lin, Alice V. McGarvey, Jeffery A. Hnasko, Robert Detection of Shiga Toxins by Lateral Flow Assay |
title | Detection of Shiga Toxins by Lateral Flow Assay |
title_full | Detection of Shiga Toxins by Lateral Flow Assay |
title_fullStr | Detection of Shiga Toxins by Lateral Flow Assay |
title_full_unstemmed | Detection of Shiga Toxins by Lateral Flow Assay |
title_short | Detection of Shiga Toxins by Lateral Flow Assay |
title_sort | detection of shiga toxins by lateral flow assay |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4417961/ https://www.ncbi.nlm.nih.gov/pubmed/25855129 http://dx.doi.org/10.3390/toxins7041163 |
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