Cargando…

Correlation between Saliva and Plasma Levels of Endothelin Isoforms ET-1, ET-2, and ET-3

Although saliva endothelins are emerging as valuable noninvasive cardiovascular biomarkers, reports on the relationship between isoforms in saliva and plasma remain scarce. We measured endothelins in concurrent saliva and plasma samples (n = 30 males; age 18–63) by HPLC-fluorescence. Results reveale...

Descripción completa

Detalles Bibliográficos
Autores principales: Gurusankar, Roma, Kumarathasan, Prem, Saravanamuthu, Anusha, Thomson, Errol M., Vincent, Renaud
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4417981/
https://www.ncbi.nlm.nih.gov/pubmed/25972900
http://dx.doi.org/10.1155/2015/828759
_version_ 1782369416636268544
author Gurusankar, Roma
Kumarathasan, Prem
Saravanamuthu, Anusha
Thomson, Errol M.
Vincent, Renaud
author_facet Gurusankar, Roma
Kumarathasan, Prem
Saravanamuthu, Anusha
Thomson, Errol M.
Vincent, Renaud
author_sort Gurusankar, Roma
collection PubMed
description Although saliva endothelins are emerging as valuable noninvasive cardiovascular biomarkers, reports on the relationship between isoforms in saliva and plasma remain scarce. We measured endothelins in concurrent saliva and plasma samples (n = 30 males; age 18–63) by HPLC-fluorescence. Results revealed statistically significant positive correlations among all isoforms between saliva and plasma: big endothelin-1 (BET-1, 0.55 ± 0.27 versus 3.35 ± 1.28 pmol/mL; r = 0.38, p = 0.041), endothelin-1 (ET-1, 0.52 ± 0.21 versus 3.45 ± 1.28 pmol/mL; r = 0.53, p = 0.003), endothelin-2 (ET-2, 0.21 ± 0.07 versus 1.63 ± 0.66 pmol/mL; r = 0.51, p = 0.004), and endothelin-3 (ET-3, 0.39 ± 0.19 versus 2.32 ± 1.44 pmol/mL; r = 0.75, p < 0.001). Correlations of BET-1, ET-1, and ET-3 within each compartment were positive in both plasma (p < 0.05) and saliva (p ≤ 0.1), whereas ET-2 was not significantly correlated with other isoforms in either plasma or saliva. For all isoforms, concentrations varied on average fivefold between individuals (90th/10th percentiles); individuals with high plasma endothelin levels generally had high saliva endothelin levels. Our results reveal that salivary ET isoform profiles portray the plasmatic profiles and support the view of coordinated regulation of ET-1 and ET-3, but distinct regulatory pathways for ET-2.
format Online
Article
Text
id pubmed-4417981
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-44179812015-05-13 Correlation between Saliva and Plasma Levels of Endothelin Isoforms ET-1, ET-2, and ET-3 Gurusankar, Roma Kumarathasan, Prem Saravanamuthu, Anusha Thomson, Errol M. Vincent, Renaud Int J Pept Research Article Although saliva endothelins are emerging as valuable noninvasive cardiovascular biomarkers, reports on the relationship between isoforms in saliva and plasma remain scarce. We measured endothelins in concurrent saliva and plasma samples (n = 30 males; age 18–63) by HPLC-fluorescence. Results revealed statistically significant positive correlations among all isoforms between saliva and plasma: big endothelin-1 (BET-1, 0.55 ± 0.27 versus 3.35 ± 1.28 pmol/mL; r = 0.38, p = 0.041), endothelin-1 (ET-1, 0.52 ± 0.21 versus 3.45 ± 1.28 pmol/mL; r = 0.53, p = 0.003), endothelin-2 (ET-2, 0.21 ± 0.07 versus 1.63 ± 0.66 pmol/mL; r = 0.51, p = 0.004), and endothelin-3 (ET-3, 0.39 ± 0.19 versus 2.32 ± 1.44 pmol/mL; r = 0.75, p < 0.001). Correlations of BET-1, ET-1, and ET-3 within each compartment were positive in both plasma (p < 0.05) and saliva (p ≤ 0.1), whereas ET-2 was not significantly correlated with other isoforms in either plasma or saliva. For all isoforms, concentrations varied on average fivefold between individuals (90th/10th percentiles); individuals with high plasma endothelin levels generally had high saliva endothelin levels. Our results reveal that salivary ET isoform profiles portray the plasmatic profiles and support the view of coordinated regulation of ET-1 and ET-3, but distinct regulatory pathways for ET-2. Hindawi Publishing Corporation 2015 2015-04-20 /pmc/articles/PMC4417981/ /pubmed/25972900 http://dx.doi.org/10.1155/2015/828759 Text en Copyright © 2015 Roma Gurusankar et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Gurusankar, Roma
Kumarathasan, Prem
Saravanamuthu, Anusha
Thomson, Errol M.
Vincent, Renaud
Correlation between Saliva and Plasma Levels of Endothelin Isoforms ET-1, ET-2, and ET-3
title Correlation between Saliva and Plasma Levels of Endothelin Isoforms ET-1, ET-2, and ET-3
title_full Correlation between Saliva and Plasma Levels of Endothelin Isoforms ET-1, ET-2, and ET-3
title_fullStr Correlation between Saliva and Plasma Levels of Endothelin Isoforms ET-1, ET-2, and ET-3
title_full_unstemmed Correlation between Saliva and Plasma Levels of Endothelin Isoforms ET-1, ET-2, and ET-3
title_short Correlation between Saliva and Plasma Levels of Endothelin Isoforms ET-1, ET-2, and ET-3
title_sort correlation between saliva and plasma levels of endothelin isoforms et-1, et-2, and et-3
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4417981/
https://www.ncbi.nlm.nih.gov/pubmed/25972900
http://dx.doi.org/10.1155/2015/828759
work_keys_str_mv AT gurusankarroma correlationbetweensalivaandplasmalevelsofendothelinisoformset1et2andet3
AT kumarathasanprem correlationbetweensalivaandplasmalevelsofendothelinisoformset1et2andet3
AT saravanamuthuanusha correlationbetweensalivaandplasmalevelsofendothelinisoformset1et2andet3
AT thomsonerrolm correlationbetweensalivaandplasmalevelsofendothelinisoformset1et2andet3
AT vincentrenaud correlationbetweensalivaandplasmalevelsofendothelinisoformset1et2andet3