Cargando…
In Vitro antibacterial and in Vivo cytotoxic activities of Grewia paniculata
OBJECTIVES: Grewia paniculata (Family: Malvaceae) has been used to treat inflammation, respiratory disorders and fever. It is additionally employed for other health conditions including colds, diarrhea and as an insecticide in Bangladesh. The aim of the present study was to investigate the antibacte...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Mashhad University of Medical Sciences
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4418058/ https://www.ncbi.nlm.nih.gov/pubmed/25949950 |
_version_ | 1782369428807090176 |
---|---|
author | Nasrin, Mahmuda Dash, Pritesh Ranjan Ali, Mohammad Shawkat |
author_facet | Nasrin, Mahmuda Dash, Pritesh Ranjan Ali, Mohammad Shawkat |
author_sort | Nasrin, Mahmuda |
collection | PubMed |
description | OBJECTIVES: Grewia paniculata (Family: Malvaceae) has been used to treat inflammation, respiratory disorders and fever. It is additionally employed for other health conditions including colds, diarrhea and as an insecticide in Bangladesh. The aim of the present study was to investigate the antibacterial and cytotoxic activities of different extracts of Grewia paniculata. MATERIALS AND METHODS: The antibacterial activity was evaluated against both gram negative and gram positive bacteria using disc diffusion method by determination of the diameter of zone of inhibition. Cytotoxic activity was performed by brine shrimp (Artemia salina) lethality bioassay. RESULTS: In disc diffusion method, all the natural products (400 μg/disc) showed moderate to potent activity against all the tested bacteria. The ethanol extract of bark (EEB) and ethanol fraction of bark (EFB) (400 μg/disc) exhibited highest activity against Shigella dysenteriae with a zone of inhibition of 23±1.63 mm and 23±1.77 mm respectively. In the brine shrimp lethality bioassay all the extracts showed moderate cytotoxic activity when compared with the standard drug vincristin sulphate. For example, LC50 value of the ethanol fraction of bark (EFB) was 3.01 μg/ml while the LC50 of vincristine sulphate was 0.52 μg/ml. CONCLUSIONS: The results suggest that all the natural products possess potent antibacterial and moderate cytotoxic. |
format | Online Article Text |
id | pubmed-4418058 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Mashhad University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-44180582015-05-06 In Vitro antibacterial and in Vivo cytotoxic activities of Grewia paniculata Nasrin, Mahmuda Dash, Pritesh Ranjan Ali, Mohammad Shawkat Avicenna J Phytomed Original Article OBJECTIVES: Grewia paniculata (Family: Malvaceae) has been used to treat inflammation, respiratory disorders and fever. It is additionally employed for other health conditions including colds, diarrhea and as an insecticide in Bangladesh. The aim of the present study was to investigate the antibacterial and cytotoxic activities of different extracts of Grewia paniculata. MATERIALS AND METHODS: The antibacterial activity was evaluated against both gram negative and gram positive bacteria using disc diffusion method by determination of the diameter of zone of inhibition. Cytotoxic activity was performed by brine shrimp (Artemia salina) lethality bioassay. RESULTS: In disc diffusion method, all the natural products (400 μg/disc) showed moderate to potent activity against all the tested bacteria. The ethanol extract of bark (EEB) and ethanol fraction of bark (EFB) (400 μg/disc) exhibited highest activity against Shigella dysenteriae with a zone of inhibition of 23±1.63 mm and 23±1.77 mm respectively. In the brine shrimp lethality bioassay all the extracts showed moderate cytotoxic activity when compared with the standard drug vincristin sulphate. For example, LC50 value of the ethanol fraction of bark (EFB) was 3.01 μg/ml while the LC50 of vincristine sulphate was 0.52 μg/ml. CONCLUSIONS: The results suggest that all the natural products possess potent antibacterial and moderate cytotoxic. Mashhad University of Medical Sciences 2015 /pmc/articles/PMC4418058/ /pubmed/25949950 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Nasrin, Mahmuda Dash, Pritesh Ranjan Ali, Mohammad Shawkat In Vitro antibacterial and in Vivo cytotoxic activities of Grewia paniculata |
title |
In Vitro antibacterial and in Vivo cytotoxic activities of Grewia paniculata |
title_full |
In Vitro antibacterial and in Vivo cytotoxic activities of Grewia paniculata |
title_fullStr |
In Vitro antibacterial and in Vivo cytotoxic activities of Grewia paniculata |
title_full_unstemmed |
In Vitro antibacterial and in Vivo cytotoxic activities of Grewia paniculata |
title_short |
In Vitro antibacterial and in Vivo cytotoxic activities of Grewia paniculata |
title_sort | in vitro antibacterial and in vivo cytotoxic activities of grewia paniculata |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4418058/ https://www.ncbi.nlm.nih.gov/pubmed/25949950 |
work_keys_str_mv | AT nasrinmahmuda invitroantibacterialandinvivocytotoxicactivitiesofgrewiapaniculata AT dashpriteshranjan invitroantibacterialandinvivocytotoxicactivitiesofgrewiapaniculata AT alimohammadshawkat invitroantibacterialandinvivocytotoxicactivitiesofgrewiapaniculata |