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Expression of inducible nitric oxide synthase in carcinomas and sarcomas affecting the oral cavity

CONTEXT: Inducible nitric oxide synthase (iNOS) is a cytoplasmic enzyme which plays a crucial role in the pathogenesis of oral carcinomas and sarcomas. AIMS: The objective of this study was to analyze the immunohistochemical expression of iNOS in carcinomas and sarcomas affecting the oral cavity in...

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Autores principales: Augustine, Dominic, Sekar, B., Murali, S., Ramesh, Maya, Madhavan, R. Nirmal, Patil, Shankar Gouda, Rao, Roopa S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4418088/
https://www.ncbi.nlm.nih.gov/pubmed/25992347
http://dx.doi.org/10.4103/2278-330X.155686
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author Augustine, Dominic
Sekar, B.
Murali, S.
Ramesh, Maya
Madhavan, R. Nirmal
Patil, Shankar Gouda
Rao, Roopa S
author_facet Augustine, Dominic
Sekar, B.
Murali, S.
Ramesh, Maya
Madhavan, R. Nirmal
Patil, Shankar Gouda
Rao, Roopa S
author_sort Augustine, Dominic
collection PubMed
description CONTEXT: Inducible nitric oxide synthase (iNOS) is a cytoplasmic enzyme which plays a crucial role in the pathogenesis of oral carcinomas and sarcomas. AIMS: The objective of this study was to analyze the immunohistochemical expression of iNOS in carcinomas and sarcomas affecting the oral cavity in order to understand the possible role of iNOS in their biologic behavior and to correlate iNOS expression with lymph node metastasis in carcinomas and sarcomas. SETTINGS AND DESIGN: Patients, who attended the oral diagnosis department of Vinayaka Missions Sankarachariyar Dental College, were screened, for the purpose of the study. Besides these, paraffin-embedded tissue blocks were also retrieved from archives of the Oral and Maxillofacial Pathology Department. A total of 40 cases (20 carcinomas and 20 sarcomas) were selected for the study. SUBJECTS AND METHODS: A total of 40 cases (20 carcinomas and 20 sarcomas) were selected for the study. Five apparently normal tissues were obtained from the tumor adjacent normal tissue to be used as a control. These were subjected to immunohistochemical staining using antibody to iNOS and evaluated. STATISTICAL ANALYSIS USED: The results were analyzed using the Chi-square test. RESULTS: Among the 20 carcinomas 19 showed a positive immunoreactivity for iNOS and 1 case was negative. Among the 19 immunopositive iNOS cases of carcinomas, 15 cases showed positive lymph node metastasis. Among the sarcomas, positive immunoreactivity for iNOS was seen in 10 hard tissue sarcomas, while the remaining 10 soft tissue sarcomas were negative for iNOS expression. The results were analyzed using the Chi-square test. CONCLUSIONS: iNOS is a reliable marker for lymph node metastasis in carcinomas irrespective of the histologic grade. The high expression in carcinomas shows that the carcinomas elaborate more angiogenesis for growth compared with the sarcomas with the exception of hard tissue sarcomas.
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spelling pubmed-44180882015-05-19 Expression of inducible nitric oxide synthase in carcinomas and sarcomas affecting the oral cavity Augustine, Dominic Sekar, B. Murali, S. Ramesh, Maya Madhavan, R. Nirmal Patil, Shankar Gouda Rao, Roopa S South Asian J Cancer MINI SYMPOSIUM: MOLECULAR ONCOLOGY CONTEXT: Inducible nitric oxide synthase (iNOS) is a cytoplasmic enzyme which plays a crucial role in the pathogenesis of oral carcinomas and sarcomas. AIMS: The objective of this study was to analyze the immunohistochemical expression of iNOS in carcinomas and sarcomas affecting the oral cavity in order to understand the possible role of iNOS in their biologic behavior and to correlate iNOS expression with lymph node metastasis in carcinomas and sarcomas. SETTINGS AND DESIGN: Patients, who attended the oral diagnosis department of Vinayaka Missions Sankarachariyar Dental College, were screened, for the purpose of the study. Besides these, paraffin-embedded tissue blocks were also retrieved from archives of the Oral and Maxillofacial Pathology Department. A total of 40 cases (20 carcinomas and 20 sarcomas) were selected for the study. SUBJECTS AND METHODS: A total of 40 cases (20 carcinomas and 20 sarcomas) were selected for the study. Five apparently normal tissues were obtained from the tumor adjacent normal tissue to be used as a control. These were subjected to immunohistochemical staining using antibody to iNOS and evaluated. STATISTICAL ANALYSIS USED: The results were analyzed using the Chi-square test. RESULTS: Among the 20 carcinomas 19 showed a positive immunoreactivity for iNOS and 1 case was negative. Among the 19 immunopositive iNOS cases of carcinomas, 15 cases showed positive lymph node metastasis. Among the sarcomas, positive immunoreactivity for iNOS was seen in 10 hard tissue sarcomas, while the remaining 10 soft tissue sarcomas were negative for iNOS expression. The results were analyzed using the Chi-square test. CONCLUSIONS: iNOS is a reliable marker for lymph node metastasis in carcinomas irrespective of the histologic grade. The high expression in carcinomas shows that the carcinomas elaborate more angiogenesis for growth compared with the sarcomas with the exception of hard tissue sarcomas. Medknow Publications & Media Pvt Ltd 2015 /pmc/articles/PMC4418088/ /pubmed/25992347 http://dx.doi.org/10.4103/2278-330X.155686 Text en Copyright: © South Asian Journal of Cancer http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle MINI SYMPOSIUM: MOLECULAR ONCOLOGY
Augustine, Dominic
Sekar, B.
Murali, S.
Ramesh, Maya
Madhavan, R. Nirmal
Patil, Shankar Gouda
Rao, Roopa S
Expression of inducible nitric oxide synthase in carcinomas and sarcomas affecting the oral cavity
title Expression of inducible nitric oxide synthase in carcinomas and sarcomas affecting the oral cavity
title_full Expression of inducible nitric oxide synthase in carcinomas and sarcomas affecting the oral cavity
title_fullStr Expression of inducible nitric oxide synthase in carcinomas and sarcomas affecting the oral cavity
title_full_unstemmed Expression of inducible nitric oxide synthase in carcinomas and sarcomas affecting the oral cavity
title_short Expression of inducible nitric oxide synthase in carcinomas and sarcomas affecting the oral cavity
title_sort expression of inducible nitric oxide synthase in carcinomas and sarcomas affecting the oral cavity
topic MINI SYMPOSIUM: MOLECULAR ONCOLOGY
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4418088/
https://www.ncbi.nlm.nih.gov/pubmed/25992347
http://dx.doi.org/10.4103/2278-330X.155686
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