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Effect of experimental stress on the small bowel and colon in healthy humans

BACKGROUND: Symptoms of irritable bowel syndrome (IBS) are frequently reported to be exacerbated by stress. Animal studies suggest that corticotrophin releasing hormone (CRH) mediates the effect of stress on the bowel. We have shown that stressed IBS patients with diarrhea have constricted small bow...

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Autores principales: Pritchard, SE, Garsed, KC, Hoad, CL, Lingaya, M, Banwait, R, Thongborisute, W, Roberts, E, Costigan, C, Marciani, L, Gowland, PA, Spiller, RC
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4418402/
https://www.ncbi.nlm.nih.gov/pubmed/25703609
http://dx.doi.org/10.1111/nmo.12529
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author Pritchard, SE
Garsed, KC
Hoad, CL
Lingaya, M
Banwait, R
Thongborisute, W
Roberts, E
Costigan, C
Marciani, L
Gowland, PA
Spiller, RC
author_facet Pritchard, SE
Garsed, KC
Hoad, CL
Lingaya, M
Banwait, R
Thongborisute, W
Roberts, E
Costigan, C
Marciani, L
Gowland, PA
Spiller, RC
author_sort Pritchard, SE
collection PubMed
description BACKGROUND: Symptoms of irritable bowel syndrome (IBS) are frequently reported to be exacerbated by stress. Animal studies suggest that corticotrophin releasing hormone (CRH) mediates the effect of stress on the bowel. We have shown that stressed IBS patients with diarrhea have constricted small bowels. We hypothesized that we could mimic this effect by applying experimental stress in the form of either hand immersion in ice water or CRH injection in healthy volunteers (HV). METHODS: The postprandial effect of the cold pressor test (repeated hand immersion in ice cold water) and injection of CRH, were assessed vs control in two groups of 18 HVs. KEY RESULTS: CRH produced a significant rise from baseline salivary cortisol levels (p = 0.004) not seen with the cold pressor test. Small bowel water content (SBWC) fell postprandially on all four treatments. SBWC was significantly reduced by both stressors but CRH caused a greater effect (anova, p < 0.003 vs p = 0.02). Ascending colon (AC) volume was greater after CRH injection compared with saline (p = 0.002) but no differences were seen with the cold pressor test vs warm water. Postprandial increase in colon volume was also reduced by CRH which also increased the sensations of distension and bloating. CONCLUSIONS & INFERENCES: Two experimental stressors were shown to constrict the small bowel, mimicking the effect previously seen in IBS-D patients. CRH increased the volume of the AC. We speculate that stress accelerates transfer of water from the small bowel to the AC.
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spelling pubmed-44184022015-05-06 Effect of experimental stress on the small bowel and colon in healthy humans Pritchard, SE Garsed, KC Hoad, CL Lingaya, M Banwait, R Thongborisute, W Roberts, E Costigan, C Marciani, L Gowland, PA Spiller, RC Neurogastroenterol Motil Original Articles BACKGROUND: Symptoms of irritable bowel syndrome (IBS) are frequently reported to be exacerbated by stress. Animal studies suggest that corticotrophin releasing hormone (CRH) mediates the effect of stress on the bowel. We have shown that stressed IBS patients with diarrhea have constricted small bowels. We hypothesized that we could mimic this effect by applying experimental stress in the form of either hand immersion in ice water or CRH injection in healthy volunteers (HV). METHODS: The postprandial effect of the cold pressor test (repeated hand immersion in ice cold water) and injection of CRH, were assessed vs control in two groups of 18 HVs. KEY RESULTS: CRH produced a significant rise from baseline salivary cortisol levels (p = 0.004) not seen with the cold pressor test. Small bowel water content (SBWC) fell postprandially on all four treatments. SBWC was significantly reduced by both stressors but CRH caused a greater effect (anova, p < 0.003 vs p = 0.02). Ascending colon (AC) volume was greater after CRH injection compared with saline (p = 0.002) but no differences were seen with the cold pressor test vs warm water. Postprandial increase in colon volume was also reduced by CRH which also increased the sensations of distension and bloating. CONCLUSIONS & INFERENCES: Two experimental stressors were shown to constrict the small bowel, mimicking the effect previously seen in IBS-D patients. CRH increased the volume of the AC. We speculate that stress accelerates transfer of water from the small bowel to the AC. Blackwell Publishing Ltd 2015-04 2015-02-20 /pmc/articles/PMC4418402/ /pubmed/25703609 http://dx.doi.org/10.1111/nmo.12529 Text en © 2015 The Authors. Neurogastroenterology & Motility published by John Wiley & Sons Ltd. http://creativecommons.org/licenses/by-nc/4.0/ This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Original Articles
Pritchard, SE
Garsed, KC
Hoad, CL
Lingaya, M
Banwait, R
Thongborisute, W
Roberts, E
Costigan, C
Marciani, L
Gowland, PA
Spiller, RC
Effect of experimental stress on the small bowel and colon in healthy humans
title Effect of experimental stress on the small bowel and colon in healthy humans
title_full Effect of experimental stress on the small bowel and colon in healthy humans
title_fullStr Effect of experimental stress on the small bowel and colon in healthy humans
title_full_unstemmed Effect of experimental stress on the small bowel and colon in healthy humans
title_short Effect of experimental stress on the small bowel and colon in healthy humans
title_sort effect of experimental stress on the small bowel and colon in healthy humans
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4418402/
https://www.ncbi.nlm.nih.gov/pubmed/25703609
http://dx.doi.org/10.1111/nmo.12529
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