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Methanolic Extract of Ficus carica Linn. Leaves Exerts Antiangiogenesis Effects Based on the Rat Air Pouch Model of Inflammation

The antiangiogenesis effect of Ficus carica leaves extract in an air pouch model of inflammation was investigated in rat. Inflammation was induced by injection of carrageenan into pouches. After antioxidant capacity and total phenolic content (TPC) investigations, the extract was administered at 5,...

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Autores principales: Eteraf-Oskouei, Tahereh, Allahyari, Saeideh, Akbarzadeh-Atashkhosrow, Arezu, Delazar, Abbas, Pashaii, Mahdiyeh, Gan, Siew Hua, Najafi, Moslem
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4419259/
https://www.ncbi.nlm.nih.gov/pubmed/25977699
http://dx.doi.org/10.1155/2015/760405
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author Eteraf-Oskouei, Tahereh
Allahyari, Saeideh
Akbarzadeh-Atashkhosrow, Arezu
Delazar, Abbas
Pashaii, Mahdiyeh
Gan, Siew Hua
Najafi, Moslem
author_facet Eteraf-Oskouei, Tahereh
Allahyari, Saeideh
Akbarzadeh-Atashkhosrow, Arezu
Delazar, Abbas
Pashaii, Mahdiyeh
Gan, Siew Hua
Najafi, Moslem
author_sort Eteraf-Oskouei, Tahereh
collection PubMed
description The antiangiogenesis effect of Ficus carica leaves extract in an air pouch model of inflammation was investigated in rat. Inflammation was induced by injection of carrageenan into pouches. After antioxidant capacity and total phenolic content (TPC) investigations, the extract was administered at 5, 25, and 50 mg/pouch, and then the volume of exudates, the cell number, TNFα, PGE(2), and VEGF levels were measured. Angiogenesis of granulation tissues was determined by measuring hemoglobin content. Based on the DPPH assay, the extract had significant antioxidant activity with TPC of 11.70 mg GAE/100 g dry sample. In addition, leukocyte accumulation and volume of exudate were significantly inhibited by the extract. Moreover, it significantly decreased the production of TNFα, PGE(2), and VEGF, while angiogenesis was significantly inhibited by all administered doses. Interestingly, attenuation of angiogenesis and inflammatory parameters (except leukocyte accumulation) by the extract was similar to that shown by diclofenac. The extract has anti-inflammatory effects and ameliorated cell influx and exudation to the site of the inflammatory response which may be related to the local inhibition of TNFα, PGE(2), and VEGF levels as similarly shown by diclofenac. The antiangiogenesis and anti-VEGF effects of Ficus carica may be correlated with its significant antioxidant potentials.
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spelling pubmed-44192592015-05-14 Methanolic Extract of Ficus carica Linn. Leaves Exerts Antiangiogenesis Effects Based on the Rat Air Pouch Model of Inflammation Eteraf-Oskouei, Tahereh Allahyari, Saeideh Akbarzadeh-Atashkhosrow, Arezu Delazar, Abbas Pashaii, Mahdiyeh Gan, Siew Hua Najafi, Moslem Evid Based Complement Alternat Med Research Article The antiangiogenesis effect of Ficus carica leaves extract in an air pouch model of inflammation was investigated in rat. Inflammation was induced by injection of carrageenan into pouches. After antioxidant capacity and total phenolic content (TPC) investigations, the extract was administered at 5, 25, and 50 mg/pouch, and then the volume of exudates, the cell number, TNFα, PGE(2), and VEGF levels were measured. Angiogenesis of granulation tissues was determined by measuring hemoglobin content. Based on the DPPH assay, the extract had significant antioxidant activity with TPC of 11.70 mg GAE/100 g dry sample. In addition, leukocyte accumulation and volume of exudate were significantly inhibited by the extract. Moreover, it significantly decreased the production of TNFα, PGE(2), and VEGF, while angiogenesis was significantly inhibited by all administered doses. Interestingly, attenuation of angiogenesis and inflammatory parameters (except leukocyte accumulation) by the extract was similar to that shown by diclofenac. The extract has anti-inflammatory effects and ameliorated cell influx and exudation to the site of the inflammatory response which may be related to the local inhibition of TNFα, PGE(2), and VEGF levels as similarly shown by diclofenac. The antiangiogenesis and anti-VEGF effects of Ficus carica may be correlated with its significant antioxidant potentials. Hindawi Publishing Corporation 2015 2015-04-21 /pmc/articles/PMC4419259/ /pubmed/25977699 http://dx.doi.org/10.1155/2015/760405 Text en Copyright © 2015 Tahereh Eteraf-Oskouei et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Eteraf-Oskouei, Tahereh
Allahyari, Saeideh
Akbarzadeh-Atashkhosrow, Arezu
Delazar, Abbas
Pashaii, Mahdiyeh
Gan, Siew Hua
Najafi, Moslem
Methanolic Extract of Ficus carica Linn. Leaves Exerts Antiangiogenesis Effects Based on the Rat Air Pouch Model of Inflammation
title Methanolic Extract of Ficus carica Linn. Leaves Exerts Antiangiogenesis Effects Based on the Rat Air Pouch Model of Inflammation
title_full Methanolic Extract of Ficus carica Linn. Leaves Exerts Antiangiogenesis Effects Based on the Rat Air Pouch Model of Inflammation
title_fullStr Methanolic Extract of Ficus carica Linn. Leaves Exerts Antiangiogenesis Effects Based on the Rat Air Pouch Model of Inflammation
title_full_unstemmed Methanolic Extract of Ficus carica Linn. Leaves Exerts Antiangiogenesis Effects Based on the Rat Air Pouch Model of Inflammation
title_short Methanolic Extract of Ficus carica Linn. Leaves Exerts Antiangiogenesis Effects Based on the Rat Air Pouch Model of Inflammation
title_sort methanolic extract of ficus carica linn. leaves exerts antiangiogenesis effects based on the rat air pouch model of inflammation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4419259/
https://www.ncbi.nlm.nih.gov/pubmed/25977699
http://dx.doi.org/10.1155/2015/760405
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