Purification and characterization of DR_2577 (SlpA) a major S-layer protein from Deinococcus radiodurans

The protein DR_2577 is a major Surface layer component of the radio-resistant bacterium Deinococcus radiodurans. In the present study DR_2577 has been purified and its oligomeric profile characterized by means of size exclusion chromatography and gel electrophoresis. DR_2577 was found to be organize...

Descripción completa

Detalles Bibliográficos
Autores principales: Farci, Domenica, Bowler, Matthew W., Esposito, Francesca, McSweeney, Sean, Tramontano, Enzo, Piano, Dario
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4419837/
https://www.ncbi.nlm.nih.gov/pubmed/26074883
http://dx.doi.org/10.3389/fmicb.2015.00414
_version_ 1782369648545628160
author Farci, Domenica
Bowler, Matthew W.
Esposito, Francesca
McSweeney, Sean
Tramontano, Enzo
Piano, Dario
author_facet Farci, Domenica
Bowler, Matthew W.
Esposito, Francesca
McSweeney, Sean
Tramontano, Enzo
Piano, Dario
author_sort Farci, Domenica
collection PubMed
description The protein DR_2577 is a major Surface layer component of the radio-resistant bacterium Deinococcus radiodurans. In the present study DR_2577 has been purified and its oligomeric profile characterized by means of size exclusion chromatography and gel electrophoresis. DR_2577 was found to be organized into three hierarchical orders characterized by monomers, stable dimers formed by the occurrence of disulfide bonds, and hexamers resulting from a combination of dimers. The structural implications of these findings are discussed providing new elements for a more integrated model of this S-layer.
format Online
Article
Text
id pubmed-4419837
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-44198372015-06-12 Purification and characterization of DR_2577 (SlpA) a major S-layer protein from Deinococcus radiodurans Farci, Domenica Bowler, Matthew W. Esposito, Francesca McSweeney, Sean Tramontano, Enzo Piano, Dario Front Microbiol Microbiology The protein DR_2577 is a major Surface layer component of the radio-resistant bacterium Deinococcus radiodurans. In the present study DR_2577 has been purified and its oligomeric profile characterized by means of size exclusion chromatography and gel electrophoresis. DR_2577 was found to be organized into three hierarchical orders characterized by monomers, stable dimers formed by the occurrence of disulfide bonds, and hexamers resulting from a combination of dimers. The structural implications of these findings are discussed providing new elements for a more integrated model of this S-layer. Frontiers Media S.A. 2015-06-03 /pmc/articles/PMC4419837/ /pubmed/26074883 http://dx.doi.org/10.3389/fmicb.2015.00414 Text en Copyright © 2015 Farci, Bowler, Esposito, McSweeney, Tramontano and Piano. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Farci, Domenica
Bowler, Matthew W.
Esposito, Francesca
McSweeney, Sean
Tramontano, Enzo
Piano, Dario
Purification and characterization of DR_2577 (SlpA) a major S-layer protein from Deinococcus radiodurans
title Purification and characterization of DR_2577 (SlpA) a major S-layer protein from Deinococcus radiodurans
title_full Purification and characterization of DR_2577 (SlpA) a major S-layer protein from Deinococcus radiodurans
title_fullStr Purification and characterization of DR_2577 (SlpA) a major S-layer protein from Deinococcus radiodurans
title_full_unstemmed Purification and characterization of DR_2577 (SlpA) a major S-layer protein from Deinococcus radiodurans
title_short Purification and characterization of DR_2577 (SlpA) a major S-layer protein from Deinococcus radiodurans
title_sort purification and characterization of dr_2577 (slpa) a major s-layer protein from deinococcus radiodurans
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4419837/
https://www.ncbi.nlm.nih.gov/pubmed/26074883
http://dx.doi.org/10.3389/fmicb.2015.00414
work_keys_str_mv AT farcidomenica purificationandcharacterizationofdr2577slpaamajorslayerproteinfromdeinococcusradiodurans
AT bowlermattheww purificationandcharacterizationofdr2577slpaamajorslayerproteinfromdeinococcusradiodurans
AT espositofrancesca purificationandcharacterizationofdr2577slpaamajorslayerproteinfromdeinococcusradiodurans
AT mcsweeneysean purificationandcharacterizationofdr2577slpaamajorslayerproteinfromdeinococcusradiodurans
AT tramontanoenzo purificationandcharacterizationofdr2577slpaamajorslayerproteinfromdeinococcusradiodurans
AT pianodario purificationandcharacterizationofdr2577slpaamajorslayerproteinfromdeinococcusradiodurans

Ejemplares similares