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Recombinant Protein Expression for Structural Biology in HEK 293F Suspension Cells: A Novel and Accessible Approach

The expression and purification of large amounts of recombinant protein complexes is an essential requirement for structural biology studies. For over two decades, prokaryotic expression systems such as E. coli have dominated the scientific literature over costly and less efficient eukaryotic cell l...

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Detalles Bibliográficos
Autores principales:	Portolano, Nicola, Watson, Peter J., Fairall, Louise, Millard, Christopher J., Milano, Charles P., Song, Yun, Cowley, Shaun M., Schwabe, John W.R.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MyJove Corporation 2014
Materias:	Biochemistry
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4420617/ https://www.ncbi.nlm.nih.gov/pubmed/25349981 http://dx.doi.org/10.3791/51897

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