Cargando…
Direct Electrochemistry of Glucose Oxidase on Novel Free-Standing Nitrogen-Doped Carbon Nanospheres@Carbon Nanofibers Composite Film
We have proposed a novel free-standing nitrogen-doped carbon nanospheres@carbon nanofibers (NCNSs@CNFs) composite film with high processability for the investigation of the direct electron transfer (DET) of glucose oxidase (GOx) and the DET-based glucose biosensing. The composites were simply prepar...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4421824/ https://www.ncbi.nlm.nih.gov/pubmed/25943704 http://dx.doi.org/10.1038/srep09885 |
_version_ | 1782369954363867136 |
---|---|
author | Zhang, Xueping Liu, Dong Li, Libo You, Tianyan |
author_facet | Zhang, Xueping Liu, Dong Li, Libo You, Tianyan |
author_sort | Zhang, Xueping |
collection | PubMed |
description | We have proposed a novel free-standing nitrogen-doped carbon nanospheres@carbon nanofibers (NCNSs@CNFs) composite film with high processability for the investigation of the direct electron transfer (DET) of glucose oxidase (GOx) and the DET-based glucose biosensing. The composites were simply prepared by controlled thermal treatment of electrospun polypyrrole nanospheres doped polyacrylonitrile nanofibers (PPyNSs@PAN NFs). Without any pretreatment, the as-prepared material can directly serve as a platform for GOx immobilization. The cyclic voltammetry of immobilized GOx showed a pair of well-defined redox peaks in O(2)-free solution, indicating the DET of GOx. With the addition of glucose, the anodic peak current increased, while the cathodic peak current decreased, which demonstrated the DET-based bioelectrocatalysis. The detection of glucose based on the DET of GOx was achieved, which displayed high sensitivity, stability and selectivity, with a low detection limit of 2 μM and wide linear range of 12–1000 μM. These results demonstrate that the as-obtained NCNSs@CNFs can serve as an ideal platform for the construction of the third-generation glucose biosensor. |
format | Online Article Text |
id | pubmed-4421824 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-44218242015-05-18 Direct Electrochemistry of Glucose Oxidase on Novel Free-Standing Nitrogen-Doped Carbon Nanospheres@Carbon Nanofibers Composite Film Zhang, Xueping Liu, Dong Li, Libo You, Tianyan Sci Rep Article We have proposed a novel free-standing nitrogen-doped carbon nanospheres@carbon nanofibers (NCNSs@CNFs) composite film with high processability for the investigation of the direct electron transfer (DET) of glucose oxidase (GOx) and the DET-based glucose biosensing. The composites were simply prepared by controlled thermal treatment of electrospun polypyrrole nanospheres doped polyacrylonitrile nanofibers (PPyNSs@PAN NFs). Without any pretreatment, the as-prepared material can directly serve as a platform for GOx immobilization. The cyclic voltammetry of immobilized GOx showed a pair of well-defined redox peaks in O(2)-free solution, indicating the DET of GOx. With the addition of glucose, the anodic peak current increased, while the cathodic peak current decreased, which demonstrated the DET-based bioelectrocatalysis. The detection of glucose based on the DET of GOx was achieved, which displayed high sensitivity, stability and selectivity, with a low detection limit of 2 μM and wide linear range of 12–1000 μM. These results demonstrate that the as-obtained NCNSs@CNFs can serve as an ideal platform for the construction of the third-generation glucose biosensor. Nature Publishing Group 2015-05-06 /pmc/articles/PMC4421824/ /pubmed/25943704 http://dx.doi.org/10.1038/srep09885 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Zhang, Xueping Liu, Dong Li, Libo You, Tianyan Direct Electrochemistry of Glucose Oxidase on Novel Free-Standing Nitrogen-Doped Carbon Nanospheres@Carbon Nanofibers Composite Film |
title | Direct Electrochemistry of Glucose Oxidase on Novel Free-Standing Nitrogen-Doped Carbon Nanospheres@Carbon Nanofibers Composite Film |
title_full | Direct Electrochemistry of Glucose Oxidase on Novel Free-Standing Nitrogen-Doped Carbon Nanospheres@Carbon Nanofibers Composite Film |
title_fullStr | Direct Electrochemistry of Glucose Oxidase on Novel Free-Standing Nitrogen-Doped Carbon Nanospheres@Carbon Nanofibers Composite Film |
title_full_unstemmed | Direct Electrochemistry of Glucose Oxidase on Novel Free-Standing Nitrogen-Doped Carbon Nanospheres@Carbon Nanofibers Composite Film |
title_short | Direct Electrochemistry of Glucose Oxidase on Novel Free-Standing Nitrogen-Doped Carbon Nanospheres@Carbon Nanofibers Composite Film |
title_sort | direct electrochemistry of glucose oxidase on novel free-standing nitrogen-doped carbon nanospheres@carbon nanofibers composite film |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4421824/ https://www.ncbi.nlm.nih.gov/pubmed/25943704 http://dx.doi.org/10.1038/srep09885 |
work_keys_str_mv | AT zhangxueping directelectrochemistryofglucoseoxidaseonnovelfreestandingnitrogendopedcarbonnanospherescarbonnanofiberscompositefilm AT liudong directelectrochemistryofglucoseoxidaseonnovelfreestandingnitrogendopedcarbonnanospherescarbonnanofiberscompositefilm AT lilibo directelectrochemistryofglucoseoxidaseonnovelfreestandingnitrogendopedcarbonnanospherescarbonnanofiberscompositefilm AT youtianyan directelectrochemistryofglucoseoxidaseonnovelfreestandingnitrogendopedcarbonnanospherescarbonnanofiberscompositefilm |