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Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders

Amide hydrogen/deuterium exchange (ssHDX-MS) and side-chain photolytic labeling (ssPL-MS) followed by mass spectrometric analysis can be valuable for characterizing lyophilized formulations of protein therapeutics. Labeling followed by suitable proteolytic digestion allows the protein structure and...

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Detalles Bibliográficos
Autores principales:	Moorthy, Balakrishnan S., Iyer, Lavanya K., Topp, Elizabeth M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MyJove Corporation 2015
Materias:	Chemistry
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4422116/ https://www.ncbi.nlm.nih.gov/pubmed/25938927 http://dx.doi.org/10.3791/52503

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