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Transgenic Alfalfa Plants Expressing the Sweetpotato Orange Gene Exhibit Enhanced Abiotic Stress Tolerance
Alfalfa (Medicago sativa L.), a perennial forage crop with high nutritional content, is widely distributed in various environments worldwide. We recently demonstrated that the sweetpotato Orange gene (IbOr) is involved in increasing carotenoid accumulation and enhancing resistance to multiple abioti...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4422619/ https://www.ncbi.nlm.nih.gov/pubmed/25946429 http://dx.doi.org/10.1371/journal.pone.0126050 |
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author | Wang, Zhi Ke, Qingbo Kim, Myoung Duck Kim, Sun Ha Ji, Chang Yoon Jeong, Jae Cheol Lee, Haeng-Soon Park, Woo Sung Ahn, Mi-Jeong Li, Hongbing Xu, Bingcheng Deng, Xiping Lee, Sang-Hoon Lim, Yong Pyo Kwak, Sang-Soo |
author_facet | Wang, Zhi Ke, Qingbo Kim, Myoung Duck Kim, Sun Ha Ji, Chang Yoon Jeong, Jae Cheol Lee, Haeng-Soon Park, Woo Sung Ahn, Mi-Jeong Li, Hongbing Xu, Bingcheng Deng, Xiping Lee, Sang-Hoon Lim, Yong Pyo Kwak, Sang-Soo |
author_sort | Wang, Zhi |
collection | PubMed |
description | Alfalfa (Medicago sativa L.), a perennial forage crop with high nutritional content, is widely distributed in various environments worldwide. We recently demonstrated that the sweetpotato Orange gene (IbOr) is involved in increasing carotenoid accumulation and enhancing resistance to multiple abiotic stresses. In this study, in an effort to improve the nutritional quality and environmental stress tolerance of alfalfa, we transferred the IbOr gene into alfalfa (cv. Xinjiang Daye) under the control of an oxidative stress-inducible peroxidase (SWPA2) promoter through Agrobacterium tumefaciens-mediated transformation. Among the 11 transgenic alfalfa lines (referred to as SOR plants), three lines (SOR2, SOR3, and SOR8) selected based on their IbOr transcript levels were examined for their tolerance to methyl viologen (MV)-induced oxidative stress in a leaf disc assay. The SOR plants exhibited less damage in response to MV-mediated oxidative stress and salt stress than non-transgenic plants. The SOR plants also exhibited enhanced tolerance to drought stress, along with higher total carotenoid levels. The results suggest that SOR alfalfa plants would be useful as forage crops with improved nutritional value and increased tolerance to multiple abiotic stresses, which would enhance the development of sustainable agriculture on marginal lands. |
format | Online Article Text |
id | pubmed-4422619 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-44226192015-05-12 Transgenic Alfalfa Plants Expressing the Sweetpotato Orange Gene Exhibit Enhanced Abiotic Stress Tolerance Wang, Zhi Ke, Qingbo Kim, Myoung Duck Kim, Sun Ha Ji, Chang Yoon Jeong, Jae Cheol Lee, Haeng-Soon Park, Woo Sung Ahn, Mi-Jeong Li, Hongbing Xu, Bingcheng Deng, Xiping Lee, Sang-Hoon Lim, Yong Pyo Kwak, Sang-Soo PLoS One Research Article Alfalfa (Medicago sativa L.), a perennial forage crop with high nutritional content, is widely distributed in various environments worldwide. We recently demonstrated that the sweetpotato Orange gene (IbOr) is involved in increasing carotenoid accumulation and enhancing resistance to multiple abiotic stresses. In this study, in an effort to improve the nutritional quality and environmental stress tolerance of alfalfa, we transferred the IbOr gene into alfalfa (cv. Xinjiang Daye) under the control of an oxidative stress-inducible peroxidase (SWPA2) promoter through Agrobacterium tumefaciens-mediated transformation. Among the 11 transgenic alfalfa lines (referred to as SOR plants), three lines (SOR2, SOR3, and SOR8) selected based on their IbOr transcript levels were examined for their tolerance to methyl viologen (MV)-induced oxidative stress in a leaf disc assay. The SOR plants exhibited less damage in response to MV-mediated oxidative stress and salt stress than non-transgenic plants. The SOR plants also exhibited enhanced tolerance to drought stress, along with higher total carotenoid levels. The results suggest that SOR alfalfa plants would be useful as forage crops with improved nutritional value and increased tolerance to multiple abiotic stresses, which would enhance the development of sustainable agriculture on marginal lands. Public Library of Science 2015-05-06 /pmc/articles/PMC4422619/ /pubmed/25946429 http://dx.doi.org/10.1371/journal.pone.0126050 Text en © 2015 Wang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Wang, Zhi Ke, Qingbo Kim, Myoung Duck Kim, Sun Ha Ji, Chang Yoon Jeong, Jae Cheol Lee, Haeng-Soon Park, Woo Sung Ahn, Mi-Jeong Li, Hongbing Xu, Bingcheng Deng, Xiping Lee, Sang-Hoon Lim, Yong Pyo Kwak, Sang-Soo Transgenic Alfalfa Plants Expressing the Sweetpotato Orange Gene Exhibit Enhanced Abiotic Stress Tolerance |
title | Transgenic Alfalfa Plants Expressing the Sweetpotato Orange Gene Exhibit Enhanced Abiotic Stress Tolerance |
title_full | Transgenic Alfalfa Plants Expressing the Sweetpotato Orange Gene Exhibit Enhanced Abiotic Stress Tolerance |
title_fullStr | Transgenic Alfalfa Plants Expressing the Sweetpotato Orange Gene Exhibit Enhanced Abiotic Stress Tolerance |
title_full_unstemmed | Transgenic Alfalfa Plants Expressing the Sweetpotato Orange Gene Exhibit Enhanced Abiotic Stress Tolerance |
title_short | Transgenic Alfalfa Plants Expressing the Sweetpotato Orange Gene Exhibit Enhanced Abiotic Stress Tolerance |
title_sort | transgenic alfalfa plants expressing the sweetpotato orange gene exhibit enhanced abiotic stress tolerance |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4422619/ https://www.ncbi.nlm.nih.gov/pubmed/25946429 http://dx.doi.org/10.1371/journal.pone.0126050 |
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