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Cellulosome stoichiometry in Clostridium cellulolyticum is regulated by selective RNA processing and stabilization

The mechanism, physiological relevance and evolutionary implication of selective RNA processing and stabilization (SRPS) remain elusive. Here we report the genome-wide maps of transcriptional start sites (TSs) and post-transcriptional processed sites (PSs) for Clostridium cellulolyticum. The PS-asso...

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Autores principales: Xu, Chenggang, Huang, Ranran, Teng, Lin, Jing, Xiaoyan, Hu, Jianqiang, Cui, Guzhen, Wang, Yilin, Cui, Qiu, Xu, Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Pub. Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4423207/
https://www.ncbi.nlm.nih.gov/pubmed/25908225
http://dx.doi.org/10.1038/ncomms7900
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author Xu, Chenggang
Huang, Ranran
Teng, Lin
Jing, Xiaoyan
Hu, Jianqiang
Cui, Guzhen
Wang, Yilin
Cui, Qiu
Xu, Jian
author_facet Xu, Chenggang
Huang, Ranran
Teng, Lin
Jing, Xiaoyan
Hu, Jianqiang
Cui, Guzhen
Wang, Yilin
Cui, Qiu
Xu, Jian
author_sort Xu, Chenggang
collection PubMed
description The mechanism, physiological relevance and evolutionary implication of selective RNA processing and stabilization (SRPS) remain elusive. Here we report the genome-wide maps of transcriptional start sites (TSs) and post-transcriptional processed sites (PSs) for Clostridium cellulolyticum. The PS-associated genes are preferably associated with subunits of heteromultimeric protein complexes, and the intergenic PSs (iPSs) are enriched in operons exhibiting highly skewed transcript-abundance landscape. Stem-loop structures associated with those iPSs located at 3′ termini of highly transcribed genes exhibit folding free energy negatively correlated with transcript-abundance ratio of flanking genes. In the cellulosome-encoding cip-cel operon, iPSs and stem-loops precisely regulate structure and abundance of the subunit-encoding transcripts processed from a primary polycistronic RNA, quantitatively specifying cellulosome stoichiometry. Moreover, cellulosome evolution is shaped by the number, position and biophysical nature of TSs, iPSs and stem-loops. Our findings unveil a genome-wide RNA-encoded strategy controlling in vivo stoichiometry of protein complexes.
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spelling pubmed-44232072015-05-20 Cellulosome stoichiometry in Clostridium cellulolyticum is regulated by selective RNA processing and stabilization Xu, Chenggang Huang, Ranran Teng, Lin Jing, Xiaoyan Hu, Jianqiang Cui, Guzhen Wang, Yilin Cui, Qiu Xu, Jian Nat Commun Article The mechanism, physiological relevance and evolutionary implication of selective RNA processing and stabilization (SRPS) remain elusive. Here we report the genome-wide maps of transcriptional start sites (TSs) and post-transcriptional processed sites (PSs) for Clostridium cellulolyticum. The PS-associated genes are preferably associated with subunits of heteromultimeric protein complexes, and the intergenic PSs (iPSs) are enriched in operons exhibiting highly skewed transcript-abundance landscape. Stem-loop structures associated with those iPSs located at 3′ termini of highly transcribed genes exhibit folding free energy negatively correlated with transcript-abundance ratio of flanking genes. In the cellulosome-encoding cip-cel operon, iPSs and stem-loops precisely regulate structure and abundance of the subunit-encoding transcripts processed from a primary polycistronic RNA, quantitatively specifying cellulosome stoichiometry. Moreover, cellulosome evolution is shaped by the number, position and biophysical nature of TSs, iPSs and stem-loops. Our findings unveil a genome-wide RNA-encoded strategy controlling in vivo stoichiometry of protein complexes. Nature Pub. Group 2015-04-24 /pmc/articles/PMC4423207/ /pubmed/25908225 http://dx.doi.org/10.1038/ncomms7900 Text en Copyright © 2015, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Xu, Chenggang
Huang, Ranran
Teng, Lin
Jing, Xiaoyan
Hu, Jianqiang
Cui, Guzhen
Wang, Yilin
Cui, Qiu
Xu, Jian
Cellulosome stoichiometry in Clostridium cellulolyticum is regulated by selective RNA processing and stabilization
title Cellulosome stoichiometry in Clostridium cellulolyticum is regulated by selective RNA processing and stabilization
title_full Cellulosome stoichiometry in Clostridium cellulolyticum is regulated by selective RNA processing and stabilization
title_fullStr Cellulosome stoichiometry in Clostridium cellulolyticum is regulated by selective RNA processing and stabilization
title_full_unstemmed Cellulosome stoichiometry in Clostridium cellulolyticum is regulated by selective RNA processing and stabilization
title_short Cellulosome stoichiometry in Clostridium cellulolyticum is regulated by selective RNA processing and stabilization
title_sort cellulosome stoichiometry in clostridium cellulolyticum is regulated by selective rna processing and stabilization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4423207/
https://www.ncbi.nlm.nih.gov/pubmed/25908225
http://dx.doi.org/10.1038/ncomms7900
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