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Single-cell evaluation of red blood cell bio-mechanical and nano-structural alterations upon chemically induced oxidative stress

Erythroid cells, specifically red blood cells (RBCs), are constantly exposed to highly reactive radicals during cellular gaseous exchange. Such exposure often exceeds the cells' innate anti-oxidant defense systems, leading to progressive damage and eventual senescence. One of the contributing f...

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Autores principales: Sinha, Ameya, Chu, Trang T. T., Dao, Ming, Chandramohanadas, Rajesh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4423428/
https://www.ncbi.nlm.nih.gov/pubmed/25950144
http://dx.doi.org/10.1038/srep09768
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author Sinha, Ameya
Chu, Trang T. T.
Dao, Ming
Chandramohanadas, Rajesh
author_facet Sinha, Ameya
Chu, Trang T. T.
Dao, Ming
Chandramohanadas, Rajesh
author_sort Sinha, Ameya
collection PubMed
description Erythroid cells, specifically red blood cells (RBCs), are constantly exposed to highly reactive radicals during cellular gaseous exchange. Such exposure often exceeds the cells' innate anti-oxidant defense systems, leading to progressive damage and eventual senescence. One of the contributing factors to this process are alterations to hemoglobin conformation and globin binding to red cell cytoskeleton. However, in addition to the aforementioned changes, it is possible that oxidative damage induces critical changes to the erythrocyte cytoskeleton and corresponding bio-mechanical and nano-structural properties of the red cell membrane. To quantitatively characterize how oxidative damage accounts for such changes, we employed single-cell manipulation techniques such as micropipette aspiration and atomic force microscopy (AFM) on RBCs. These investigations demonstrated visible morphological changes upon chemically induced oxidative damage (using hydrogen peroxide, diamide, primaquine bisphosphate and cumene hydroperoxide). Our results provide previously unavailable observations on remarkable changes in red cell cytoskeletal architecture and membrane stiffness due to oxidative damage. Furthermore, we also demonstrate that a pathogen that infects human blood cells, Plasmodium falciparum was unable to penetrate through the oxidant-exposed RBCs that have damaged cytoskeleton and stiffer membranes. This indicates the importance of bio-physical factors pertinent to aged RBCs and it's relevance to malaria infectivity.
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spelling pubmed-44234282015-05-13 Single-cell evaluation of red blood cell bio-mechanical and nano-structural alterations upon chemically induced oxidative stress Sinha, Ameya Chu, Trang T. T. Dao, Ming Chandramohanadas, Rajesh Sci Rep Article Erythroid cells, specifically red blood cells (RBCs), are constantly exposed to highly reactive radicals during cellular gaseous exchange. Such exposure often exceeds the cells' innate anti-oxidant defense systems, leading to progressive damage and eventual senescence. One of the contributing factors to this process are alterations to hemoglobin conformation and globin binding to red cell cytoskeleton. However, in addition to the aforementioned changes, it is possible that oxidative damage induces critical changes to the erythrocyte cytoskeleton and corresponding bio-mechanical and nano-structural properties of the red cell membrane. To quantitatively characterize how oxidative damage accounts for such changes, we employed single-cell manipulation techniques such as micropipette aspiration and atomic force microscopy (AFM) on RBCs. These investigations demonstrated visible morphological changes upon chemically induced oxidative damage (using hydrogen peroxide, diamide, primaquine bisphosphate and cumene hydroperoxide). Our results provide previously unavailable observations on remarkable changes in red cell cytoskeletal architecture and membrane stiffness due to oxidative damage. Furthermore, we also demonstrate that a pathogen that infects human blood cells, Plasmodium falciparum was unable to penetrate through the oxidant-exposed RBCs that have damaged cytoskeleton and stiffer membranes. This indicates the importance of bio-physical factors pertinent to aged RBCs and it's relevance to malaria infectivity. Nature Publishing Group 2015-05-07 /pmc/articles/PMC4423428/ /pubmed/25950144 http://dx.doi.org/10.1038/srep09768 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Sinha, Ameya
Chu, Trang T. T.
Dao, Ming
Chandramohanadas, Rajesh
Single-cell evaluation of red blood cell bio-mechanical and nano-structural alterations upon chemically induced oxidative stress
title Single-cell evaluation of red blood cell bio-mechanical and nano-structural alterations upon chemically induced oxidative stress
title_full Single-cell evaluation of red blood cell bio-mechanical and nano-structural alterations upon chemically induced oxidative stress
title_fullStr Single-cell evaluation of red blood cell bio-mechanical and nano-structural alterations upon chemically induced oxidative stress
title_full_unstemmed Single-cell evaluation of red blood cell bio-mechanical and nano-structural alterations upon chemically induced oxidative stress
title_short Single-cell evaluation of red blood cell bio-mechanical and nano-structural alterations upon chemically induced oxidative stress
title_sort single-cell evaluation of red blood cell bio-mechanical and nano-structural alterations upon chemically induced oxidative stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4423428/
https://www.ncbi.nlm.nih.gov/pubmed/25950144
http://dx.doi.org/10.1038/srep09768
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