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Common occurrence of antibacterial agents in human intestinal microbiota

Laboratory experiments have revealed many active mechanisms by which bacteria can inhibit the growth of other organisms. Bacteriocins are a diverse group of natural ribosomally synthesized antimicrobial peptides produced by a wide range of bacteria and which seem to play an important role in mediati...

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Autores principales: Drissi, Fatima, Buffet, Sylvain, Raoult, Didier, Merhej, Vicky
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4423438/
https://www.ncbi.nlm.nih.gov/pubmed/25999943
http://dx.doi.org/10.3389/fmicb.2015.00441
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author Drissi, Fatima
Buffet, Sylvain
Raoult, Didier
Merhej, Vicky
author_facet Drissi, Fatima
Buffet, Sylvain
Raoult, Didier
Merhej, Vicky
author_sort Drissi, Fatima
collection PubMed
description Laboratory experiments have revealed many active mechanisms by which bacteria can inhibit the growth of other organisms. Bacteriocins are a diverse group of natural ribosomally synthesized antimicrobial peptides produced by a wide range of bacteria and which seem to play an important role in mediating competition within bacterial communities. In this study, we have identified and established the structural classification of putative bacteriocins encoded by 317 microbial genomes in the human intestine. On the basis of homologies to available bacteriocin sequences, mainly from lactic acid bacteria, we report the widespread occurrence of bacteriocins across the gut microbiota: 175 bacteriocins were found to be encoded in Firmicutes, 79 in Proteobacteria, 34 in Bacteroidetes, and 25 in Actinobacteria. Bacteriocins from gut bacteria displayed wide differences among phyla with regard to class distribution, net positive charge, hydrophobicity and secondary structure, but the α-helix was the most abundant structure. The peptide structures and physiochemical properties of bacteriocins produced by the most abundant bacteria in the gut, the Firmicutes and the Bacteroidetes, seem to ensure low antibiotic activity and participate in permanent intestinal host defense against the proliferation of harmful bacteria. Meanwhile, the potentially harmful bacteria, including the Proteobacteria, displayed highly effective bacteriocins, probably supporting the virulent character of diseases. These findings highlight the eventual role played by bacteriocins in gut microbial competition and their potential place in antibiotic therapy.
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spelling pubmed-44234382015-05-21 Common occurrence of antibacterial agents in human intestinal microbiota Drissi, Fatima Buffet, Sylvain Raoult, Didier Merhej, Vicky Front Microbiol Microbiology Laboratory experiments have revealed many active mechanisms by which bacteria can inhibit the growth of other organisms. Bacteriocins are a diverse group of natural ribosomally synthesized antimicrobial peptides produced by a wide range of bacteria and which seem to play an important role in mediating competition within bacterial communities. In this study, we have identified and established the structural classification of putative bacteriocins encoded by 317 microbial genomes in the human intestine. On the basis of homologies to available bacteriocin sequences, mainly from lactic acid bacteria, we report the widespread occurrence of bacteriocins across the gut microbiota: 175 bacteriocins were found to be encoded in Firmicutes, 79 in Proteobacteria, 34 in Bacteroidetes, and 25 in Actinobacteria. Bacteriocins from gut bacteria displayed wide differences among phyla with regard to class distribution, net positive charge, hydrophobicity and secondary structure, but the α-helix was the most abundant structure. The peptide structures and physiochemical properties of bacteriocins produced by the most abundant bacteria in the gut, the Firmicutes and the Bacteroidetes, seem to ensure low antibiotic activity and participate in permanent intestinal host defense against the proliferation of harmful bacteria. Meanwhile, the potentially harmful bacteria, including the Proteobacteria, displayed highly effective bacteriocins, probably supporting the virulent character of diseases. These findings highlight the eventual role played by bacteriocins in gut microbial competition and their potential place in antibiotic therapy. Frontiers Media S.A. 2015-05-07 /pmc/articles/PMC4423438/ /pubmed/25999943 http://dx.doi.org/10.3389/fmicb.2015.00441 Text en Copyright © 2015 Drissi, Buffet, Raoult and Merhej. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Drissi, Fatima
Buffet, Sylvain
Raoult, Didier
Merhej, Vicky
Common occurrence of antibacterial agents in human intestinal microbiota
title Common occurrence of antibacterial agents in human intestinal microbiota
title_full Common occurrence of antibacterial agents in human intestinal microbiota
title_fullStr Common occurrence of antibacterial agents in human intestinal microbiota
title_full_unstemmed Common occurrence of antibacterial agents in human intestinal microbiota
title_short Common occurrence of antibacterial agents in human intestinal microbiota
title_sort common occurrence of antibacterial agents in human intestinal microbiota
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4423438/
https://www.ncbi.nlm.nih.gov/pubmed/25999943
http://dx.doi.org/10.3389/fmicb.2015.00441
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