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Ca-Mediated Electroformation of Cell-Sized Lipid Vesicles
Cell-sized lipid giant unilamellar vesicles (GUVs) are formed when lipid molecules self-assemble to construct a single bilayer compartment with similar morphology to living cells. The physics of self-assembly process is only generally understood and the size distribution of GUVs tends to be very pol...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4423497/ https://www.ncbi.nlm.nih.gov/pubmed/25950604 http://dx.doi.org/10.1038/srep09839 |
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author | Tao, Fei Yang, Peng |
author_facet | Tao, Fei Yang, Peng |
author_sort | Tao, Fei |
collection | PubMed |
description | Cell-sized lipid giant unilamellar vesicles (GUVs) are formed when lipid molecules self-assemble to construct a single bilayer compartment with similar morphology to living cells. The physics of self-assembly process is only generally understood and the size distribution of GUVs tends to be very polydisperse. Herein we report a strategy for the production of controlled size distributions of GUVs by a novel mechanism dissecting the mediation ability of calcium (Ca) on the conventional electroformation of GUVs. We finely construct both of the calcium ion (Ca(2+)) and calcium carbonate (CaCO(3)) mineral adsorption layers on a lipid film surface respectively during the electroformation of GUVs. It is found that Ca(2+) Slip plane polarized by alternating electric field could induce a pattern of electroosmotic flow across the surface, and thus confine the fusion and growth of GUVs to facilitate the formation of uniform GUVs. The model is further improved by directly using CaCO(3) that is in situ formed on a lipid film surface, providing a GUV population with narrow polydispersity. The two models deciphers the new biological function of calcium on the birth of cell-like lipid vesicles, and thus might be potentially relevant to the construction of new model to elucidate the cellular development process. |
format | Online Article Text |
id | pubmed-4423497 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-44234972015-05-13 Ca-Mediated Electroformation of Cell-Sized Lipid Vesicles Tao, Fei Yang, Peng Sci Rep Article Cell-sized lipid giant unilamellar vesicles (GUVs) are formed when lipid molecules self-assemble to construct a single bilayer compartment with similar morphology to living cells. The physics of self-assembly process is only generally understood and the size distribution of GUVs tends to be very polydisperse. Herein we report a strategy for the production of controlled size distributions of GUVs by a novel mechanism dissecting the mediation ability of calcium (Ca) on the conventional electroformation of GUVs. We finely construct both of the calcium ion (Ca(2+)) and calcium carbonate (CaCO(3)) mineral adsorption layers on a lipid film surface respectively during the electroformation of GUVs. It is found that Ca(2+) Slip plane polarized by alternating electric field could induce a pattern of electroosmotic flow across the surface, and thus confine the fusion and growth of GUVs to facilitate the formation of uniform GUVs. The model is further improved by directly using CaCO(3) that is in situ formed on a lipid film surface, providing a GUV population with narrow polydispersity. The two models deciphers the new biological function of calcium on the birth of cell-like lipid vesicles, and thus might be potentially relevant to the construction of new model to elucidate the cellular development process. Nature Publishing Group 2015-05-07 /pmc/articles/PMC4423497/ /pubmed/25950604 http://dx.doi.org/10.1038/srep09839 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Tao, Fei Yang, Peng Ca-Mediated Electroformation of Cell-Sized Lipid Vesicles |
title | Ca-Mediated Electroformation of Cell-Sized Lipid Vesicles |
title_full | Ca-Mediated Electroformation of Cell-Sized Lipid Vesicles |
title_fullStr | Ca-Mediated Electroformation of Cell-Sized Lipid Vesicles |
title_full_unstemmed | Ca-Mediated Electroformation of Cell-Sized Lipid Vesicles |
title_short | Ca-Mediated Electroformation of Cell-Sized Lipid Vesicles |
title_sort | ca-mediated electroformation of cell-sized lipid vesicles |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4423497/ https://www.ncbi.nlm.nih.gov/pubmed/25950604 http://dx.doi.org/10.1038/srep09839 |
work_keys_str_mv | AT taofei camediatedelectroformationofcellsizedlipidvesicles AT yangpeng camediatedelectroformationofcellsizedlipidvesicles |