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Function of glutathione peroxidases in legume root nodules

Glutathione peroxidases (Gpxs) are antioxidant enzymes not studied so far in legume nodules, despite the fact that reactive oxygen species are produced at different steps of the symbiosis. The function of two Gpxs that are highly expressed in nodules of the model legume Lotus japonicus was examined....

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Autores principales: Matamoros, Manuel A., Saiz, Ana, Peñuelas, Maria, Bustos-Sanmamed, Pilar, Mulet, Jose M., Barja, Maria V., Rouhier, Nicolas, Moore, Marten, James, Euan K., Dietz, Karl-Josef, Becana, Manuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4423513/
https://www.ncbi.nlm.nih.gov/pubmed/25740929
http://dx.doi.org/10.1093/jxb/erv066
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author Matamoros, Manuel A.
Saiz, Ana
Peñuelas, Maria
Bustos-Sanmamed, Pilar
Mulet, Jose M.
Barja, Maria V.
Rouhier, Nicolas
Moore, Marten
James, Euan K.
Dietz, Karl-Josef
Becana, Manuel
author_facet Matamoros, Manuel A.
Saiz, Ana
Peñuelas, Maria
Bustos-Sanmamed, Pilar
Mulet, Jose M.
Barja, Maria V.
Rouhier, Nicolas
Moore, Marten
James, Euan K.
Dietz, Karl-Josef
Becana, Manuel
author_sort Matamoros, Manuel A.
collection PubMed
description Glutathione peroxidases (Gpxs) are antioxidant enzymes not studied so far in legume nodules, despite the fact that reactive oxygen species are produced at different steps of the symbiosis. The function of two Gpxs that are highly expressed in nodules of the model legume Lotus japonicus was examined. Gene expression analysis, enzymatic and nitrosylation assays, yeast cell complementation, in situ mRNA hybridization, immunoelectron microscopy, and LjGpx-green fluorescent protein (GFP) fusions were used to characterize the enzymes and to localize each transcript and isoform in nodules. The LjGpx1 and LjGpx3 genes encode thioredoxin-dependent phospholipid hydroperoxidases and are differentially regulated in response to nitric oxide (NO) and hormones. LjGpx1 and LjGpx3 are nitrosylated in vitro or in plants treated with S-nitrosoglutathione (GSNO). Consistent with the modification of the peroxidatic cysteine of LjGpx3, in vitro assays demonstrated that this modification results in enzyme inhibition. The enzymes are highly expressed in the infected zone, but the LjGpx3 mRNA is also detected in the cortex and vascular bundles. LjGpx1 is localized to the plastids and nuclei, and LjGpx3 to the cytosol and endoplasmic reticulum. Based on yeast complementation experiments, both enzymes protect against oxidative stress, salt stress, and membrane damage. It is concluded that both LjGpxs perform major antioxidative functions in nodules, preventing lipid peroxidation and other oxidative processes at different subcellular sites of vascular and infected cells. The enzymes are probably involved in hormone and NO signalling, and may be regulated through nitrosylation of the peroxidatic cysteine essential for catalytic function.
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spelling pubmed-44235132015-05-13 Function of glutathione peroxidases in legume root nodules Matamoros, Manuel A. Saiz, Ana Peñuelas, Maria Bustos-Sanmamed, Pilar Mulet, Jose M. Barja, Maria V. Rouhier, Nicolas Moore, Marten James, Euan K. Dietz, Karl-Josef Becana, Manuel J Exp Bot Research Paper Glutathione peroxidases (Gpxs) are antioxidant enzymes not studied so far in legume nodules, despite the fact that reactive oxygen species are produced at different steps of the symbiosis. The function of two Gpxs that are highly expressed in nodules of the model legume Lotus japonicus was examined. Gene expression analysis, enzymatic and nitrosylation assays, yeast cell complementation, in situ mRNA hybridization, immunoelectron microscopy, and LjGpx-green fluorescent protein (GFP) fusions were used to characterize the enzymes and to localize each transcript and isoform in nodules. The LjGpx1 and LjGpx3 genes encode thioredoxin-dependent phospholipid hydroperoxidases and are differentially regulated in response to nitric oxide (NO) and hormones. LjGpx1 and LjGpx3 are nitrosylated in vitro or in plants treated with S-nitrosoglutathione (GSNO). Consistent with the modification of the peroxidatic cysteine of LjGpx3, in vitro assays demonstrated that this modification results in enzyme inhibition. The enzymes are highly expressed in the infected zone, but the LjGpx3 mRNA is also detected in the cortex and vascular bundles. LjGpx1 is localized to the plastids and nuclei, and LjGpx3 to the cytosol and endoplasmic reticulum. Based on yeast complementation experiments, both enzymes protect against oxidative stress, salt stress, and membrane damage. It is concluded that both LjGpxs perform major antioxidative functions in nodules, preventing lipid peroxidation and other oxidative processes at different subcellular sites of vascular and infected cells. The enzymes are probably involved in hormone and NO signalling, and may be regulated through nitrosylation of the peroxidatic cysteine essential for catalytic function. Oxford University Press 2015-05 2015-03-04 /pmc/articles/PMC4423513/ /pubmed/25740929 http://dx.doi.org/10.1093/jxb/erv066 Text en © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Matamoros, Manuel A.
Saiz, Ana
Peñuelas, Maria
Bustos-Sanmamed, Pilar
Mulet, Jose M.
Barja, Maria V.
Rouhier, Nicolas
Moore, Marten
James, Euan K.
Dietz, Karl-Josef
Becana, Manuel
Function of glutathione peroxidases in legume root nodules
title Function of glutathione peroxidases in legume root nodules
title_full Function of glutathione peroxidases in legume root nodules
title_fullStr Function of glutathione peroxidases in legume root nodules
title_full_unstemmed Function of glutathione peroxidases in legume root nodules
title_short Function of glutathione peroxidases in legume root nodules
title_sort function of glutathione peroxidases in legume root nodules
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4423513/
https://www.ncbi.nlm.nih.gov/pubmed/25740929
http://dx.doi.org/10.1093/jxb/erv066
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