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Characterization of Brachypodium distachyon as a nonhost model against switchgrass rust pathogen Puccinia emaculata

BACKGROUND: Switchgrass rust, caused by Puccinia emaculata, is an important disease of switchgrass, a potential biofuel crop in the United States. In severe cases, switchgrass rust has the potential to significantly affect biomass yield. In an effort to identify novel sources of resistance against s...

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Autores principales: Gill, Upinder S, Uppalapati, Srinivasa R, Nakashima, Jin, Mysore, Kirankumar S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4424542/
https://www.ncbi.nlm.nih.gov/pubmed/25953307
http://dx.doi.org/10.1186/s12870-015-0502-9
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author Gill, Upinder S
Uppalapati, Srinivasa R
Nakashima, Jin
Mysore, Kirankumar S
author_facet Gill, Upinder S
Uppalapati, Srinivasa R
Nakashima, Jin
Mysore, Kirankumar S
author_sort Gill, Upinder S
collection PubMed
description BACKGROUND: Switchgrass rust, caused by Puccinia emaculata, is an important disease of switchgrass, a potential biofuel crop in the United States. In severe cases, switchgrass rust has the potential to significantly affect biomass yield. In an effort to identify novel sources of resistance against switchgrass rust, we explored nonhost resistance against P. emaculata by characterizing its interactions with six monocot nonhost plant species. We also studied the genetic variations for resistance among Brachypodium inbred accessions and the involvement of various defense pathways in nonhost resistance of Brachypodium. RESULTS: We characterized P. emaculata interactions with six monocot nonhost species and identified Brachypodium distachyon (Bd21) as a suitable nonhost model to study switchgrass rust. Interestingly, screening of Brachypodium accessions identified natural variations in resistance to switchgrass rust. Brachypodium inbred accessions Bd3-1 and Bd30-1 were identified as most and least resistant to switchgrass rust, respectively, when compared to tested accessions. Transcript profiling of defense-related genes indicated that the genes which were induced in Bd21after P. emaculata inoculation also had higher basal transcript abundance in Bd3-1 when compared to Bd30-1 and Bd21 indicating their potential involvement in nonhost resistance against switchgrass rust. CONCLUSION: In the present study, we identified Brachypodium as a suitable nonhost model to study switchgrass rust which exhibit type I nonhost resistance. Variations in resistance response were also observed among tested Brachypodium accessions. Brachypodium nonhost resistance against P. emaculata may involve various defense pathways as indicated by transcript profiling of defense related genes. Overall, this study provides a new avenue to utilize novel sources of nonhost resistance in Brachypodium against switchgrass rust. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12870-015-0502-9) contains supplementary material, which is available to authorized users.
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spelling pubmed-44245422015-05-09 Characterization of Brachypodium distachyon as a nonhost model against switchgrass rust pathogen Puccinia emaculata Gill, Upinder S Uppalapati, Srinivasa R Nakashima, Jin Mysore, Kirankumar S BMC Plant Biol Research Article BACKGROUND: Switchgrass rust, caused by Puccinia emaculata, is an important disease of switchgrass, a potential biofuel crop in the United States. In severe cases, switchgrass rust has the potential to significantly affect biomass yield. In an effort to identify novel sources of resistance against switchgrass rust, we explored nonhost resistance against P. emaculata by characterizing its interactions with six monocot nonhost plant species. We also studied the genetic variations for resistance among Brachypodium inbred accessions and the involvement of various defense pathways in nonhost resistance of Brachypodium. RESULTS: We characterized P. emaculata interactions with six monocot nonhost species and identified Brachypodium distachyon (Bd21) as a suitable nonhost model to study switchgrass rust. Interestingly, screening of Brachypodium accessions identified natural variations in resistance to switchgrass rust. Brachypodium inbred accessions Bd3-1 and Bd30-1 were identified as most and least resistant to switchgrass rust, respectively, when compared to tested accessions. Transcript profiling of defense-related genes indicated that the genes which were induced in Bd21after P. emaculata inoculation also had higher basal transcript abundance in Bd3-1 when compared to Bd30-1 and Bd21 indicating their potential involvement in nonhost resistance against switchgrass rust. CONCLUSION: In the present study, we identified Brachypodium as a suitable nonhost model to study switchgrass rust which exhibit type I nonhost resistance. Variations in resistance response were also observed among tested Brachypodium accessions. Brachypodium nonhost resistance against P. emaculata may involve various defense pathways as indicated by transcript profiling of defense related genes. Overall, this study provides a new avenue to utilize novel sources of nonhost resistance in Brachypodium against switchgrass rust. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12870-015-0502-9) contains supplementary material, which is available to authorized users. BioMed Central 2015-05-08 /pmc/articles/PMC4424542/ /pubmed/25953307 http://dx.doi.org/10.1186/s12870-015-0502-9 Text en © Gill et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Gill, Upinder S
Uppalapati, Srinivasa R
Nakashima, Jin
Mysore, Kirankumar S
Characterization of Brachypodium distachyon as a nonhost model against switchgrass rust pathogen Puccinia emaculata
title Characterization of Brachypodium distachyon as a nonhost model against switchgrass rust pathogen Puccinia emaculata
title_full Characterization of Brachypodium distachyon as a nonhost model against switchgrass rust pathogen Puccinia emaculata
title_fullStr Characterization of Brachypodium distachyon as a nonhost model against switchgrass rust pathogen Puccinia emaculata
title_full_unstemmed Characterization of Brachypodium distachyon as a nonhost model against switchgrass rust pathogen Puccinia emaculata
title_short Characterization of Brachypodium distachyon as a nonhost model against switchgrass rust pathogen Puccinia emaculata
title_sort characterization of brachypodium distachyon as a nonhost model against switchgrass rust pathogen puccinia emaculata
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4424542/
https://www.ncbi.nlm.nih.gov/pubmed/25953307
http://dx.doi.org/10.1186/s12870-015-0502-9
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