Protein kinase A signaling and calcium ions are major players in PAF mediated toxicity against Aspergillus niger

The Penicillium chrysogenum antifungal protein PAF is toxic against potentially pathogenic Ascomycetes. We used the highly sensitive aequorin-expressing model Aspergillus niger to identify a defined change in cytoplasmic free Ca(2+) dynamics in response to PAF. This Ca(2+) signature depended on an i...

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Detalles Bibliográficos
Autores principales: Binder, Ulrike, Benčina, Mojca, Fizil, Ádám, Batta, Gyula, Chhillar, Anil K., Marx, Florentine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Science B.V 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4424949/
https://www.ncbi.nlm.nih.gov/pubmed/25882631
http://dx.doi.org/10.1016/j.febslet.2015.03.037
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author Binder, Ulrike
Benčina, Mojca
Fizil, Ádám
Batta, Gyula
Chhillar, Anil K.
Marx, Florentine
author_facet Binder, Ulrike
Benčina, Mojca
Fizil, Ádám
Batta, Gyula
Chhillar, Anil K.
Marx, Florentine
author_sort Binder, Ulrike
collection PubMed
description The Penicillium chrysogenum antifungal protein PAF is toxic against potentially pathogenic Ascomycetes. We used the highly sensitive aequorin-expressing model Aspergillus niger to identify a defined change in cytoplasmic free Ca(2+) dynamics in response to PAF. This Ca(2+) signature depended on an intact positively charged lysine-rich PAF motif. By combining Ca(2+) measurements in A. niger mutants with deregulated cAMP/protein kinase A (PKA) signaling, we proved the interconnection of Ca(2+) perturbation and cAMP/PKA signaling in the mechanistic function of PAF. A deep understanding of the mode of action of PAF is an invaluable prerequisite for its future application as new antifungal drug.
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spelling pubmed-44249492015-05-13 Protein kinase A signaling and calcium ions are major players in PAF mediated toxicity against Aspergillus niger Binder, Ulrike Benčina, Mojca Fizil, Ádám Batta, Gyula Chhillar, Anil K. Marx, Florentine FEBS Lett Article The Penicillium chrysogenum antifungal protein PAF is toxic against potentially pathogenic Ascomycetes. We used the highly sensitive aequorin-expressing model Aspergillus niger to identify a defined change in cytoplasmic free Ca(2+) dynamics in response to PAF. This Ca(2+) signature depended on an intact positively charged lysine-rich PAF motif. By combining Ca(2+) measurements in A. niger mutants with deregulated cAMP/protein kinase A (PKA) signaling, we proved the interconnection of Ca(2+) perturbation and cAMP/PKA signaling in the mechanistic function of PAF. A deep understanding of the mode of action of PAF is an invaluable prerequisite for its future application as new antifungal drug. Elsevier Science B.V 2015-05-08 /pmc/articles/PMC4424949/ /pubmed/25882631 http://dx.doi.org/10.1016/j.febslet.2015.03.037 Text en © 2015 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Binder, Ulrike
Benčina, Mojca
Fizil, Ádám
Batta, Gyula
Chhillar, Anil K.
Marx, Florentine
Protein kinase A signaling and calcium ions are major players in PAF mediated toxicity against Aspergillus niger
title Protein kinase A signaling and calcium ions are major players in PAF mediated toxicity against Aspergillus niger
title_full Protein kinase A signaling and calcium ions are major players in PAF mediated toxicity against Aspergillus niger
title_fullStr Protein kinase A signaling and calcium ions are major players in PAF mediated toxicity against Aspergillus niger
title_full_unstemmed Protein kinase A signaling and calcium ions are major players in PAF mediated toxicity against Aspergillus niger
title_short Protein kinase A signaling and calcium ions are major players in PAF mediated toxicity against Aspergillus niger
title_sort protein kinase a signaling and calcium ions are major players in paf mediated toxicity against aspergillus niger
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4424949/
https://www.ncbi.nlm.nih.gov/pubmed/25882631
http://dx.doi.org/10.1016/j.febslet.2015.03.037
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