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Study of Linkage between Glutathione Pathway and the Antibiotic Resistance of Escherichia coli from Patients’ Swabs

In this work, we focused on the differences between bacterial cultures of E. coli obtained from swabs of infectious wounds of patients compared to laboratory E. coli. In addition, blocking of the protein responsible for the synthesis of glutathione (γ-glutamylcysteine synthase—GCL) using 10 mM buthi...

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Autores principales: Kominkova, Marketa, Michalek, Petr, Cihalova, Kristyna, Guran, Roman, Cernei, Natalia, Nejdl, Lukas, Smerkova, Kristyna, Dostalova, Simona, Chudobova, Dagmar, Heger, Zbynek, Vesely, Radek, Gumulec, Jaromir, Kynicky, Jindrich, Xhaxhiu, Kledi, Zitka, Ondrej, Adam, Vojtech, Kizek, Rene
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4425013/
https://www.ncbi.nlm.nih.gov/pubmed/25837469
http://dx.doi.org/10.3390/ijms16047210
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author Kominkova, Marketa
Michalek, Petr
Cihalova, Kristyna
Guran, Roman
Cernei, Natalia
Nejdl, Lukas
Smerkova, Kristyna
Dostalova, Simona
Chudobova, Dagmar
Heger, Zbynek
Vesely, Radek
Gumulec, Jaromir
Kynicky, Jindrich
Xhaxhiu, Kledi
Zitka, Ondrej
Adam, Vojtech
Kizek, Rene
author_facet Kominkova, Marketa
Michalek, Petr
Cihalova, Kristyna
Guran, Roman
Cernei, Natalia
Nejdl, Lukas
Smerkova, Kristyna
Dostalova, Simona
Chudobova, Dagmar
Heger, Zbynek
Vesely, Radek
Gumulec, Jaromir
Kynicky, Jindrich
Xhaxhiu, Kledi
Zitka, Ondrej
Adam, Vojtech
Kizek, Rene
author_sort Kominkova, Marketa
collection PubMed
description In this work, we focused on the differences between bacterial cultures of E. coli obtained from swabs of infectious wounds of patients compared to laboratory E. coli. In addition, blocking of the protein responsible for the synthesis of glutathione (γ-glutamylcysteine synthase—GCL) using 10 mM buthionine sulfoximine was investigated. Each E. coli showed significant differences in resistance to antibiotics. According to the determined resistance, E. coli were divided into experimental groups based on a statistical evaluation of their properties as more resistant and more sensitive. These groups were also used for finding the differences in a dependence of the glutathione pathway on resistance to antibiotics. More sensitive E. coli showed the same kinetics of glutathione synthesis while blocking GCL (K(m) 0.1 µM), as compared to non-blocking. In addition, the most frequent mutations in genes of glutathione synthetase, glutathione peroxidase and glutathione reductase were observed in this group compared to laboratory E.coli. The group of “more resistant” E. coli exhibited differences in K(m) between 0.3 and 0.8 µM. The number of mutations compared to the laboratory E. coli was substantially lower compared to the other group.
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spelling pubmed-44250132015-05-20 Study of Linkage between Glutathione Pathway and the Antibiotic Resistance of Escherichia coli from Patients’ Swabs Kominkova, Marketa Michalek, Petr Cihalova, Kristyna Guran, Roman Cernei, Natalia Nejdl, Lukas Smerkova, Kristyna Dostalova, Simona Chudobova, Dagmar Heger, Zbynek Vesely, Radek Gumulec, Jaromir Kynicky, Jindrich Xhaxhiu, Kledi Zitka, Ondrej Adam, Vojtech Kizek, Rene Int J Mol Sci Article In this work, we focused on the differences between bacterial cultures of E. coli obtained from swabs of infectious wounds of patients compared to laboratory E. coli. In addition, blocking of the protein responsible for the synthesis of glutathione (γ-glutamylcysteine synthase—GCL) using 10 mM buthionine sulfoximine was investigated. Each E. coli showed significant differences in resistance to antibiotics. According to the determined resistance, E. coli were divided into experimental groups based on a statistical evaluation of their properties as more resistant and more sensitive. These groups were also used for finding the differences in a dependence of the glutathione pathway on resistance to antibiotics. More sensitive E. coli showed the same kinetics of glutathione synthesis while blocking GCL (K(m) 0.1 µM), as compared to non-blocking. In addition, the most frequent mutations in genes of glutathione synthetase, glutathione peroxidase and glutathione reductase were observed in this group compared to laboratory E.coli. The group of “more resistant” E. coli exhibited differences in K(m) between 0.3 and 0.8 µM. The number of mutations compared to the laboratory E. coli was substantially lower compared to the other group. MDPI 2015-03-31 /pmc/articles/PMC4425013/ /pubmed/25837469 http://dx.doi.org/10.3390/ijms16047210 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kominkova, Marketa
Michalek, Petr
Cihalova, Kristyna
Guran, Roman
Cernei, Natalia
Nejdl, Lukas
Smerkova, Kristyna
Dostalova, Simona
Chudobova, Dagmar
Heger, Zbynek
Vesely, Radek
Gumulec, Jaromir
Kynicky, Jindrich
Xhaxhiu, Kledi
Zitka, Ondrej
Adam, Vojtech
Kizek, Rene
Study of Linkage between Glutathione Pathway and the Antibiotic Resistance of Escherichia coli from Patients’ Swabs
title Study of Linkage between Glutathione Pathway and the Antibiotic Resistance of Escherichia coli from Patients’ Swabs
title_full Study of Linkage between Glutathione Pathway and the Antibiotic Resistance of Escherichia coli from Patients’ Swabs
title_fullStr Study of Linkage between Glutathione Pathway and the Antibiotic Resistance of Escherichia coli from Patients’ Swabs
title_full_unstemmed Study of Linkage between Glutathione Pathway and the Antibiotic Resistance of Escherichia coli from Patients’ Swabs
title_short Study of Linkage between Glutathione Pathway and the Antibiotic Resistance of Escherichia coli from Patients’ Swabs
title_sort study of linkage between glutathione pathway and the antibiotic resistance of escherichia coli from patients’ swabs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4425013/
https://www.ncbi.nlm.nih.gov/pubmed/25837469
http://dx.doi.org/10.3390/ijms16047210
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