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Development of an Efficient Electroporation Method for Iturin A-Producing Bacillus subtilis ZK
In order to efficiently introduce DNA into B. subtilis ZK, which produces iturin A at a high level, we optimized seven electroporation conditions and explored an efficient electroporation method. Using the optimal conditions, the electroporation efficiency was improved to 1.03 × 10(7) transformants/...
Autores principales: | Zhang, Zhi, Ding, Zhong-Tao, Shu, Dan, Luo, Di, Tan, Hong |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4425020/ https://www.ncbi.nlm.nih.gov/pubmed/25837631 http://dx.doi.org/10.3390/ijms16047334 |
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