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Genome-Wide Expression in Visceral Adipose Tissue from Obese Prepubertal Children

Characterization of the genes expressed in adipose tissue (AT) is key to understanding the pathogenesis of obesity and to developing treatments for this condition. Our objective was to compare the gene expression in visceral AT (VAT) between obese and normal-weight prepubertal children. A total of f...

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Autores principales: Aguilera, Concepción M., Gomez-Llorente, Carolina, Tofe, Inés, Gil-Campos, Mercedes, Cañete, Ramón, Gil, Ángel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4425045/
https://www.ncbi.nlm.nih.gov/pubmed/25856673
http://dx.doi.org/10.3390/ijms16047723
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author Aguilera, Concepción M.
Gomez-Llorente, Carolina
Tofe, Inés
Gil-Campos, Mercedes
Cañete, Ramón
Gil, Ángel
author_facet Aguilera, Concepción M.
Gomez-Llorente, Carolina
Tofe, Inés
Gil-Campos, Mercedes
Cañete, Ramón
Gil, Ángel
author_sort Aguilera, Concepción M.
collection PubMed
description Characterization of the genes expressed in adipose tissue (AT) is key to understanding the pathogenesis of obesity and to developing treatments for this condition. Our objective was to compare the gene expression in visceral AT (VAT) between obese and normal-weight prepubertal children. A total of fifteen obese and sixteen normal-weight children undergoing abdominal elective surgery were selected. RNA was extracted from VAT biopsies. Microarray experiments were independently performed for each sample (six obese and five normal-weight samples). Validation by quantitative PCR (qPCR) was performed on an additional 10 obese and 10 normal-weight VAT samples. Of 1276 differentially expressed genes (p < 0.05), 245 were more than two-fold higher in obese children than in normal-weight children. As validated by qPCR, expression was upregulated in genes involved in lipid and amino acid metabolism (CES1, NPRR3 and BHMT2), oxidative stress and extracellular matrix regulation (TNMD and NQO1), adipogenesis (CRYAB and AFF1) and inflammation (ANXA1); by contrast, only CALCRL gene expression was confirmed to be downregulated. In conclusion, this study in prepubertal children demonstrates the up- and down-regulation of genes that encode molecules that were previously proposed to influence the pathogenesis of adulthood obesity, as well as previously unreported dysregulated genes that may be candidate genes in the aetiology of obesity.
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spelling pubmed-44250452015-05-20 Genome-Wide Expression in Visceral Adipose Tissue from Obese Prepubertal Children Aguilera, Concepción M. Gomez-Llorente, Carolina Tofe, Inés Gil-Campos, Mercedes Cañete, Ramón Gil, Ángel Int J Mol Sci Article Characterization of the genes expressed in adipose tissue (AT) is key to understanding the pathogenesis of obesity and to developing treatments for this condition. Our objective was to compare the gene expression in visceral AT (VAT) between obese and normal-weight prepubertal children. A total of fifteen obese and sixteen normal-weight children undergoing abdominal elective surgery were selected. RNA was extracted from VAT biopsies. Microarray experiments were independently performed for each sample (six obese and five normal-weight samples). Validation by quantitative PCR (qPCR) was performed on an additional 10 obese and 10 normal-weight VAT samples. Of 1276 differentially expressed genes (p < 0.05), 245 were more than two-fold higher in obese children than in normal-weight children. As validated by qPCR, expression was upregulated in genes involved in lipid and amino acid metabolism (CES1, NPRR3 and BHMT2), oxidative stress and extracellular matrix regulation (TNMD and NQO1), adipogenesis (CRYAB and AFF1) and inflammation (ANXA1); by contrast, only CALCRL gene expression was confirmed to be downregulated. In conclusion, this study in prepubertal children demonstrates the up- and down-regulation of genes that encode molecules that were previously proposed to influence the pathogenesis of adulthood obesity, as well as previously unreported dysregulated genes that may be candidate genes in the aetiology of obesity. MDPI 2015-04-08 /pmc/articles/PMC4425045/ /pubmed/25856673 http://dx.doi.org/10.3390/ijms16047723 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Aguilera, Concepción M.
Gomez-Llorente, Carolina
Tofe, Inés
Gil-Campos, Mercedes
Cañete, Ramón
Gil, Ángel
Genome-Wide Expression in Visceral Adipose Tissue from Obese Prepubertal Children
title Genome-Wide Expression in Visceral Adipose Tissue from Obese Prepubertal Children
title_full Genome-Wide Expression in Visceral Adipose Tissue from Obese Prepubertal Children
title_fullStr Genome-Wide Expression in Visceral Adipose Tissue from Obese Prepubertal Children
title_full_unstemmed Genome-Wide Expression in Visceral Adipose Tissue from Obese Prepubertal Children
title_short Genome-Wide Expression in Visceral Adipose Tissue from Obese Prepubertal Children
title_sort genome-wide expression in visceral adipose tissue from obese prepubertal children
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4425045/
https://www.ncbi.nlm.nih.gov/pubmed/25856673
http://dx.doi.org/10.3390/ijms16047723
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