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Antimicrobial Action of Water-Soluble β-Chitosan against Clinical Multi-Drug Resistant Bacteria

Recently, the number of patients infected by drug-resistant pathogenic microbes has increased remarkably worldwide, and a number of studies have reported new antibiotics from natural sources. Among them, chitosan, with a high molecular weight and α-conformation, exhibits potent antimicrobial activit...

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Autores principales: Park, Seong-Cheol, Nam, Joung-Pyo, Kim, Jun-Ho, Kim, Young-Min, Nah, Jae-Woon, Jang, Mi-Kyeong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4425063/
https://www.ncbi.nlm.nih.gov/pubmed/25867474
http://dx.doi.org/10.3390/ijms16047995
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author Park, Seong-Cheol
Nam, Joung-Pyo
Kim, Jun-Ho
Kim, Young-Min
Nah, Jae-Woon
Jang, Mi-Kyeong
author_facet Park, Seong-Cheol
Nam, Joung-Pyo
Kim, Jun-Ho
Kim, Young-Min
Nah, Jae-Woon
Jang, Mi-Kyeong
author_sort Park, Seong-Cheol
collection PubMed
description Recently, the number of patients infected by drug-resistant pathogenic microbes has increased remarkably worldwide, and a number of studies have reported new antibiotics from natural sources. Among them, chitosan, with a high molecular weight and α-conformation, exhibits potent antimicrobial activity, but useful applications as an antibiotic are limited by its cytotoxicity and insolubility at physiological pH. In the present study, the antibacterial activity of low molecular weight water-soluble (LMWS) α-chitosan (α1k, α5k, and α10k with molecular masses of 1, 5, and 10 kDa, respectively) and β-chitosan (β1k, β5k, and β10k) was compared using a range of pathogenic bacteria containing drug-resistant bacteria isolated from patients at different pH. Interestingly, β5k and β10k exhibited potent antibacterial activity, even at pH 7.4, whereas only α10k was effective at pH 7.4. The active target of β-chitosan is the bacterial membrane, where the leakage of calcein is induced in artificial PE/PG vesicles, bacterial mimetic membrane. Moreover, scanning electron microscopy showed that they caused significant morphological changes on the bacterial surfaces. An in vivo study utilizing a bacteria-infected mouse model found that LMWS β-chitosan could be used as a candidate in anti-infective or wound healing therapeutic applications.
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spelling pubmed-44250632015-05-20 Antimicrobial Action of Water-Soluble β-Chitosan against Clinical Multi-Drug Resistant Bacteria Park, Seong-Cheol Nam, Joung-Pyo Kim, Jun-Ho Kim, Young-Min Nah, Jae-Woon Jang, Mi-Kyeong Int J Mol Sci Article Recently, the number of patients infected by drug-resistant pathogenic microbes has increased remarkably worldwide, and a number of studies have reported new antibiotics from natural sources. Among them, chitosan, with a high molecular weight and α-conformation, exhibits potent antimicrobial activity, but useful applications as an antibiotic are limited by its cytotoxicity and insolubility at physiological pH. In the present study, the antibacterial activity of low molecular weight water-soluble (LMWS) α-chitosan (α1k, α5k, and α10k with molecular masses of 1, 5, and 10 kDa, respectively) and β-chitosan (β1k, β5k, and β10k) was compared using a range of pathogenic bacteria containing drug-resistant bacteria isolated from patients at different pH. Interestingly, β5k and β10k exhibited potent antibacterial activity, even at pH 7.4, whereas only α10k was effective at pH 7.4. The active target of β-chitosan is the bacterial membrane, where the leakage of calcein is induced in artificial PE/PG vesicles, bacterial mimetic membrane. Moreover, scanning electron microscopy showed that they caused significant morphological changes on the bacterial surfaces. An in vivo study utilizing a bacteria-infected mouse model found that LMWS β-chitosan could be used as a candidate in anti-infective or wound healing therapeutic applications. MDPI 2015-04-10 /pmc/articles/PMC4425063/ /pubmed/25867474 http://dx.doi.org/10.3390/ijms16047995 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Park, Seong-Cheol
Nam, Joung-Pyo
Kim, Jun-Ho
Kim, Young-Min
Nah, Jae-Woon
Jang, Mi-Kyeong
Antimicrobial Action of Water-Soluble β-Chitosan against Clinical Multi-Drug Resistant Bacteria
title Antimicrobial Action of Water-Soluble β-Chitosan against Clinical Multi-Drug Resistant Bacteria
title_full Antimicrobial Action of Water-Soluble β-Chitosan against Clinical Multi-Drug Resistant Bacteria
title_fullStr Antimicrobial Action of Water-Soluble β-Chitosan against Clinical Multi-Drug Resistant Bacteria
title_full_unstemmed Antimicrobial Action of Water-Soluble β-Chitosan against Clinical Multi-Drug Resistant Bacteria
title_short Antimicrobial Action of Water-Soluble β-Chitosan against Clinical Multi-Drug Resistant Bacteria
title_sort antimicrobial action of water-soluble β-chitosan against clinical multi-drug resistant bacteria
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4425063/
https://www.ncbi.nlm.nih.gov/pubmed/25867474
http://dx.doi.org/10.3390/ijms16047995
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