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Characterization of the Recognition Specificity of BH2, a Monoclonal Antibody Prepared against the HLA-B27 Heavy Chain

BH2, a monoclonal antibody prepared against the denatured human leukocytic antigen-B27 heavy chain (HLA-B27 HC), can immunoprecipitate the misfolded HLA-B27 HC complexed with Bip in the endoplasmic reticulum and recognize the homodimerized HLA-B27 HC that is often observed on the cell membrane of pa...

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Autores principales: Yu, Hui-Chun, Huang, Kuang-Yung, Lu, Ming-Chi, Huang, Hsien-Lu, Liu, Wei-Ting, Lee, Wen-Chien, Liu, Su-Qin, Huang, Hsien-Bin, Lai, Ning-Sheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4425072/
https://www.ncbi.nlm.nih.gov/pubmed/25872138
http://dx.doi.org/10.3390/ijms16048142
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author Yu, Hui-Chun
Huang, Kuang-Yung
Lu, Ming-Chi
Huang, Hsien-Lu
Liu, Wei-Ting
Lee, Wen-Chien
Liu, Su-Qin
Huang, Hsien-Bin
Lai, Ning-Sheng
author_facet Yu, Hui-Chun
Huang, Kuang-Yung
Lu, Ming-Chi
Huang, Hsien-Lu
Liu, Wei-Ting
Lee, Wen-Chien
Liu, Su-Qin
Huang, Hsien-Bin
Lai, Ning-Sheng
author_sort Yu, Hui-Chun
collection PubMed
description BH2, a monoclonal antibody prepared against the denatured human leukocytic antigen-B27 heavy chain (HLA-B27 HC), can immunoprecipitate the misfolded HLA-B27 HC complexed with Bip in the endoplasmic reticulum and recognize the homodimerized HLA-B27 HC that is often observed on the cell membrane of patients suffered from ankylosing spondylitis (AS). However, the recognition specificity of BH2 toward the other molecules of HLA-B type and toward the different types of HLA molecules remained uncharacterized. In this study, we carried out the HLA-typing by using the Luminex Technology to characterize the recognition specificity of BH2 and analyzed the binding domain of HLA-B27 HC by BH2. Our results indicated that BH2 preferably binds to molecules of HLA-B and -C rather than HLA-A and the binding site is located within the α2 domain of HLA-B27 HC.
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spelling pubmed-44250722015-05-20 Characterization of the Recognition Specificity of BH2, a Monoclonal Antibody Prepared against the HLA-B27 Heavy Chain Yu, Hui-Chun Huang, Kuang-Yung Lu, Ming-Chi Huang, Hsien-Lu Liu, Wei-Ting Lee, Wen-Chien Liu, Su-Qin Huang, Hsien-Bin Lai, Ning-Sheng Int J Mol Sci Communication BH2, a monoclonal antibody prepared against the denatured human leukocytic antigen-B27 heavy chain (HLA-B27 HC), can immunoprecipitate the misfolded HLA-B27 HC complexed with Bip in the endoplasmic reticulum and recognize the homodimerized HLA-B27 HC that is often observed on the cell membrane of patients suffered from ankylosing spondylitis (AS). However, the recognition specificity of BH2 toward the other molecules of HLA-B type and toward the different types of HLA molecules remained uncharacterized. In this study, we carried out the HLA-typing by using the Luminex Technology to characterize the recognition specificity of BH2 and analyzed the binding domain of HLA-B27 HC by BH2. Our results indicated that BH2 preferably binds to molecules of HLA-B and -C rather than HLA-A and the binding site is located within the α2 domain of HLA-B27 HC. MDPI 2015-04-13 /pmc/articles/PMC4425072/ /pubmed/25872138 http://dx.doi.org/10.3390/ijms16048142 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Yu, Hui-Chun
Huang, Kuang-Yung
Lu, Ming-Chi
Huang, Hsien-Lu
Liu, Wei-Ting
Lee, Wen-Chien
Liu, Su-Qin
Huang, Hsien-Bin
Lai, Ning-Sheng
Characterization of the Recognition Specificity of BH2, a Monoclonal Antibody Prepared against the HLA-B27 Heavy Chain
title Characterization of the Recognition Specificity of BH2, a Monoclonal Antibody Prepared against the HLA-B27 Heavy Chain
title_full Characterization of the Recognition Specificity of BH2, a Monoclonal Antibody Prepared against the HLA-B27 Heavy Chain
title_fullStr Characterization of the Recognition Specificity of BH2, a Monoclonal Antibody Prepared against the HLA-B27 Heavy Chain
title_full_unstemmed Characterization of the Recognition Specificity of BH2, a Monoclonal Antibody Prepared against the HLA-B27 Heavy Chain
title_short Characterization of the Recognition Specificity of BH2, a Monoclonal Antibody Prepared against the HLA-B27 Heavy Chain
title_sort characterization of the recognition specificity of bh2, a monoclonal antibody prepared against the hla-b27 heavy chain
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4425072/
https://www.ncbi.nlm.nih.gov/pubmed/25872138
http://dx.doi.org/10.3390/ijms16048142
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