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Melanogenesis-Inducing Effect of Cirsimaritin through Increases in Microphthalmia-Associated Transcription Factor and Tyrosinase Expression

The melanin-inducing properties of cirsimaritin were investigated in murine B16F10 cells. Cirsimaritin is an active flavone with methoxy groups, which is isolated from the branches of Lithocarpus dealbatus. Tyrosinase activity and melanin content in murine B16F10 melanoma cells were increased by cir...

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Autores principales: Kim, Hyo Jung, Kim, Il Soon, Dong, Yin, Lee, Ik-Soo, Kim, Jin Sook, Kim, Jong-Sang, Woo, Je-Tae, Cha, Byung-Yoon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4425108/
https://www.ncbi.nlm.nih.gov/pubmed/25903150
http://dx.doi.org/10.3390/ijms16048772
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author Kim, Hyo Jung
Kim, Il Soon
Dong, Yin
Lee, Ik-Soo
Kim, Jin Sook
Kim, Jong-Sang
Woo, Je-Tae
Cha, Byung-Yoon
author_facet Kim, Hyo Jung
Kim, Il Soon
Dong, Yin
Lee, Ik-Soo
Kim, Jin Sook
Kim, Jong-Sang
Woo, Je-Tae
Cha, Byung-Yoon
author_sort Kim, Hyo Jung
collection PubMed
description The melanin-inducing properties of cirsimaritin were investigated in murine B16F10 cells. Cirsimaritin is an active flavone with methoxy groups, which is isolated from the branches of Lithocarpus dealbatus. Tyrosinase activity and melanin content in murine B16F10 melanoma cells were increased by cirsimaritin in a dose-dependent manner. Western blot analysis revealed that tyrosinase, tyrosinase-related protein (TRP) 1, TRP2 protein levels were enhanced after treatment with cirsimaritin for 48 h. Cirsimaritin also upregulated the expression of microphthalmia-associated transcription factor (MITF) after 24 h of treatment. Furthermore, cirsimaritin induced phosphorylation of cyclic adenosine monophosphate (cAMP) response element-binding protein (CREB) in a dose-dependent manner after treatment for 15 min. The cirsimaritin-mediated increase of tyrosinase activity was significantly attenuated by H89, a cAMP-dependent protein kinase A inhibitor. These findings indicate that cirsimaritin stimulates melanogenesis in B16F10 cells by activation of CREB as well as upregulation of MITF and tyrosinase expression, which was activated by cAMP signaling. Finally, the melanogenic effect of cirsimaritin was confirmed in human epidermal melanocytes. These results support the putative application of cirsimaritin in ultraviolet photoprotection and hair coloration treatments.
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spelling pubmed-44251082015-05-20 Melanogenesis-Inducing Effect of Cirsimaritin through Increases in Microphthalmia-Associated Transcription Factor and Tyrosinase Expression Kim, Hyo Jung Kim, Il Soon Dong, Yin Lee, Ik-Soo Kim, Jin Sook Kim, Jong-Sang Woo, Je-Tae Cha, Byung-Yoon Int J Mol Sci Article The melanin-inducing properties of cirsimaritin were investigated in murine B16F10 cells. Cirsimaritin is an active flavone with methoxy groups, which is isolated from the branches of Lithocarpus dealbatus. Tyrosinase activity and melanin content in murine B16F10 melanoma cells were increased by cirsimaritin in a dose-dependent manner. Western blot analysis revealed that tyrosinase, tyrosinase-related protein (TRP) 1, TRP2 protein levels were enhanced after treatment with cirsimaritin for 48 h. Cirsimaritin also upregulated the expression of microphthalmia-associated transcription factor (MITF) after 24 h of treatment. Furthermore, cirsimaritin induced phosphorylation of cyclic adenosine monophosphate (cAMP) response element-binding protein (CREB) in a dose-dependent manner after treatment for 15 min. The cirsimaritin-mediated increase of tyrosinase activity was significantly attenuated by H89, a cAMP-dependent protein kinase A inhibitor. These findings indicate that cirsimaritin stimulates melanogenesis in B16F10 cells by activation of CREB as well as upregulation of MITF and tyrosinase expression, which was activated by cAMP signaling. Finally, the melanogenic effect of cirsimaritin was confirmed in human epidermal melanocytes. These results support the putative application of cirsimaritin in ultraviolet photoprotection and hair coloration treatments. MDPI 2015-04-20 /pmc/articles/PMC4425108/ /pubmed/25903150 http://dx.doi.org/10.3390/ijms16048772 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Hyo Jung
Kim, Il Soon
Dong, Yin
Lee, Ik-Soo
Kim, Jin Sook
Kim, Jong-Sang
Woo, Je-Tae
Cha, Byung-Yoon
Melanogenesis-Inducing Effect of Cirsimaritin through Increases in Microphthalmia-Associated Transcription Factor and Tyrosinase Expression
title Melanogenesis-Inducing Effect of Cirsimaritin through Increases in Microphthalmia-Associated Transcription Factor and Tyrosinase Expression
title_full Melanogenesis-Inducing Effect of Cirsimaritin through Increases in Microphthalmia-Associated Transcription Factor and Tyrosinase Expression
title_fullStr Melanogenesis-Inducing Effect of Cirsimaritin through Increases in Microphthalmia-Associated Transcription Factor and Tyrosinase Expression
title_full_unstemmed Melanogenesis-Inducing Effect of Cirsimaritin through Increases in Microphthalmia-Associated Transcription Factor and Tyrosinase Expression
title_short Melanogenesis-Inducing Effect of Cirsimaritin through Increases in Microphthalmia-Associated Transcription Factor and Tyrosinase Expression
title_sort melanogenesis-inducing effect of cirsimaritin through increases in microphthalmia-associated transcription factor and tyrosinase expression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4425108/
https://www.ncbi.nlm.nih.gov/pubmed/25903150
http://dx.doi.org/10.3390/ijms16048772
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