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Cytotoxic Activity of Piper cubeba Extract in Breast Cancer Cell Lines

This study aimed to evaluate the cytotoxicity of a crude extract of Piper cubeba against normal and breast cancer cell lines. To prepare the extract, P. cubeba seeds were ground, soaked in methanol and dichloromethane and isolated by column chromatography. Fractions were tested for cytotoxicity effe...

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Detalles Bibliográficos
Autores principales: Graidist, Potchanapond, Martla, Mananya, Sukpondma, Yaowapa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4425168/
https://www.ncbi.nlm.nih.gov/pubmed/25867951
http://dx.doi.org/10.3390/nu7042707
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author Graidist, Potchanapond
Martla, Mananya
Sukpondma, Yaowapa
author_facet Graidist, Potchanapond
Martla, Mananya
Sukpondma, Yaowapa
author_sort Graidist, Potchanapond
collection PubMed
description This study aimed to evaluate the cytotoxicity of a crude extract of Piper cubeba against normal and breast cancer cell lines. To prepare the extract, P. cubeba seeds were ground, soaked in methanol and dichloromethane and isolated by column chromatography. Fractions were tested for cytotoxicity effects on normal fibroblast (L929), normal breast (MCF-12A) and breast cancer cell lines (MCF-7, MDA-MB-468 and MDA-MB-231). The most effective fraction was selected for DNA fragmentation assay to detect apoptotic activity. The results showed that the methanolic crude extract had a higher cytotoxic activity against MDA-MB-468 and MCF-7 than a dichloromethane crude extract. Then, the methanolic crude extract was separated into six fractions, designated A to F. Fraction C was highly active against breast cancer cell lines with an IC(50) value less than 4 μg/mL. Therefore, Fraction C was further separated into seven fractions, CA to CG. The (1)H-NMR profile showed that Fraction CE was long chain hydrocarbons. Moreover, Fraction CE demonstrated the highest activity against MCF-7 cells with an IC(50) value of 2.69 ± 0.09 μg/mL and lower cytotoxicity against normal fibroblast L929 cells with an IC(50) value of 4.17 ± 0.77 μg/mL. Finally, DNA fragmentation with a ladder pattern characteristic of apoptosis was observed in MCF-7, MDA-MB-468, MDA-MB-231 and L929 cells, but not in MCF-12A cells.
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spelling pubmed-44251682015-05-11 Cytotoxic Activity of Piper cubeba Extract in Breast Cancer Cell Lines Graidist, Potchanapond Martla, Mananya Sukpondma, Yaowapa Nutrients Article This study aimed to evaluate the cytotoxicity of a crude extract of Piper cubeba against normal and breast cancer cell lines. To prepare the extract, P. cubeba seeds were ground, soaked in methanol and dichloromethane and isolated by column chromatography. Fractions were tested for cytotoxicity effects on normal fibroblast (L929), normal breast (MCF-12A) and breast cancer cell lines (MCF-7, MDA-MB-468 and MDA-MB-231). The most effective fraction was selected for DNA fragmentation assay to detect apoptotic activity. The results showed that the methanolic crude extract had a higher cytotoxic activity against MDA-MB-468 and MCF-7 than a dichloromethane crude extract. Then, the methanolic crude extract was separated into six fractions, designated A to F. Fraction C was highly active against breast cancer cell lines with an IC(50) value less than 4 μg/mL. Therefore, Fraction C was further separated into seven fractions, CA to CG. The (1)H-NMR profile showed that Fraction CE was long chain hydrocarbons. Moreover, Fraction CE demonstrated the highest activity against MCF-7 cells with an IC(50) value of 2.69 ± 0.09 μg/mL and lower cytotoxicity against normal fibroblast L929 cells with an IC(50) value of 4.17 ± 0.77 μg/mL. Finally, DNA fragmentation with a ladder pattern characteristic of apoptosis was observed in MCF-7, MDA-MB-468, MDA-MB-231 and L929 cells, but not in MCF-12A cells. MDPI 2015-04-10 /pmc/articles/PMC4425168/ /pubmed/25867951 http://dx.doi.org/10.3390/nu7042707 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Graidist, Potchanapond
Martla, Mananya
Sukpondma, Yaowapa
Cytotoxic Activity of Piper cubeba Extract in Breast Cancer Cell Lines
title Cytotoxic Activity of Piper cubeba Extract in Breast Cancer Cell Lines
title_full Cytotoxic Activity of Piper cubeba Extract in Breast Cancer Cell Lines
title_fullStr Cytotoxic Activity of Piper cubeba Extract in Breast Cancer Cell Lines
title_full_unstemmed Cytotoxic Activity of Piper cubeba Extract in Breast Cancer Cell Lines
title_short Cytotoxic Activity of Piper cubeba Extract in Breast Cancer Cell Lines
title_sort cytotoxic activity of piper cubeba extract in breast cancer cell lines
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4425168/
https://www.ncbi.nlm.nih.gov/pubmed/25867951
http://dx.doi.org/10.3390/nu7042707
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