Cargando…

Hydrolysis of the Rutinose-Conjugates Flavonoids Rutin and Hesperidin by the Gut Microbiota and Bifidobacteria

Flavonols and flavanones are polyphenols exerting many healthy biological activities. They are often glycosylated by rutinose, which hampers absorption in the small intestine. Therefore they require the gut microbiota to release the aglycone and enable colonic absorption. The role of the gut microbi...

Descripción completa

Detalles Bibliográficos
Autores principales: Amaretti, Alberto, Raimondi, Stefano, Leonardi, Alan, Quartieri, Andrea, Rossi, Maddalena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4425173/
https://www.ncbi.nlm.nih.gov/pubmed/25875120
http://dx.doi.org/10.3390/nu7042788
_version_ 1782370457112018944
author Amaretti, Alberto
Raimondi, Stefano
Leonardi, Alan
Quartieri, Andrea
Rossi, Maddalena
author_facet Amaretti, Alberto
Raimondi, Stefano
Leonardi, Alan
Quartieri, Andrea
Rossi, Maddalena
author_sort Amaretti, Alberto
collection PubMed
description Flavonols and flavanones are polyphenols exerting many healthy biological activities. They are often glycosylated by rutinose, which hampers absorption in the small intestine. Therefore they require the gut microbiota to release the aglycone and enable colonic absorption. The role of the gut microbiota and bifidobacteria in the release of the aglycones from two major rutinosides, hesperidin and rutin, was investigated. In bioconversion experiments, the microbiota removed rutinose from both rutin and hesperidin, even though complete hydrolysis was not obtained. To investigate whether bifidobacteria can participate to the hydrolysis of rutinosides, 33 strains were screened. Rutin was resistant to hydrolysis by all the strains. Among six tested species, mostly Bifidobacterium catenulatum and Bifidobacterium pseudocatenultum were able to hydrolyze hesperidin, by means of a cell-associated activity. This result is in agreement with the presence of a putative α-l-rhamnosidase in the genome of B. pseudocatenulatum, while most of the available genome sequences of bifidobacteria aside from this species do not bear this sequence. Even though B. pseudocatenulatum may contribute to the release of the aglycone from certain rutinose-conjugated polyphenols, such as hesperidin, it remains to be clarified whether this species may exert a role in affecting the bioavailability of the rutinoside in vivo.
format Online
Article
Text
id pubmed-4425173
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-44251732015-05-11 Hydrolysis of the Rutinose-Conjugates Flavonoids Rutin and Hesperidin by the Gut Microbiota and Bifidobacteria Amaretti, Alberto Raimondi, Stefano Leonardi, Alan Quartieri, Andrea Rossi, Maddalena Nutrients Article Flavonols and flavanones are polyphenols exerting many healthy biological activities. They are often glycosylated by rutinose, which hampers absorption in the small intestine. Therefore they require the gut microbiota to release the aglycone and enable colonic absorption. The role of the gut microbiota and bifidobacteria in the release of the aglycones from two major rutinosides, hesperidin and rutin, was investigated. In bioconversion experiments, the microbiota removed rutinose from both rutin and hesperidin, even though complete hydrolysis was not obtained. To investigate whether bifidobacteria can participate to the hydrolysis of rutinosides, 33 strains were screened. Rutin was resistant to hydrolysis by all the strains. Among six tested species, mostly Bifidobacterium catenulatum and Bifidobacterium pseudocatenultum were able to hydrolyze hesperidin, by means of a cell-associated activity. This result is in agreement with the presence of a putative α-l-rhamnosidase in the genome of B. pseudocatenulatum, while most of the available genome sequences of bifidobacteria aside from this species do not bear this sequence. Even though B. pseudocatenulatum may contribute to the release of the aglycone from certain rutinose-conjugated polyphenols, such as hesperidin, it remains to be clarified whether this species may exert a role in affecting the bioavailability of the rutinoside in vivo. MDPI 2015-04-14 /pmc/articles/PMC4425173/ /pubmed/25875120 http://dx.doi.org/10.3390/nu7042788 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Amaretti, Alberto
Raimondi, Stefano
Leonardi, Alan
Quartieri, Andrea
Rossi, Maddalena
Hydrolysis of the Rutinose-Conjugates Flavonoids Rutin and Hesperidin by the Gut Microbiota and Bifidobacteria
title Hydrolysis of the Rutinose-Conjugates Flavonoids Rutin and Hesperidin by the Gut Microbiota and Bifidobacteria
title_full Hydrolysis of the Rutinose-Conjugates Flavonoids Rutin and Hesperidin by the Gut Microbiota and Bifidobacteria
title_fullStr Hydrolysis of the Rutinose-Conjugates Flavonoids Rutin and Hesperidin by the Gut Microbiota and Bifidobacteria
title_full_unstemmed Hydrolysis of the Rutinose-Conjugates Flavonoids Rutin and Hesperidin by the Gut Microbiota and Bifidobacteria
title_short Hydrolysis of the Rutinose-Conjugates Flavonoids Rutin and Hesperidin by the Gut Microbiota and Bifidobacteria
title_sort hydrolysis of the rutinose-conjugates flavonoids rutin and hesperidin by the gut microbiota and bifidobacteria
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4425173/
https://www.ncbi.nlm.nih.gov/pubmed/25875120
http://dx.doi.org/10.3390/nu7042788
work_keys_str_mv AT amarettialberto hydrolysisoftherutinoseconjugatesflavonoidsrutinandhesperidinbythegutmicrobiotaandbifidobacteria
AT raimondistefano hydrolysisoftherutinoseconjugatesflavonoidsrutinandhesperidinbythegutmicrobiotaandbifidobacteria
AT leonardialan hydrolysisoftherutinoseconjugatesflavonoidsrutinandhesperidinbythegutmicrobiotaandbifidobacteria
AT quartieriandrea hydrolysisoftherutinoseconjugatesflavonoidsrutinandhesperidinbythegutmicrobiotaandbifidobacteria
AT rossimaddalena hydrolysisoftherutinoseconjugatesflavonoidsrutinandhesperidinbythegutmicrobiotaandbifidobacteria