Cargando…
Hydrolysis of the Rutinose-Conjugates Flavonoids Rutin and Hesperidin by the Gut Microbiota and Bifidobacteria
Flavonols and flavanones are polyphenols exerting many healthy biological activities. They are often glycosylated by rutinose, which hampers absorption in the small intestine. Therefore they require the gut microbiota to release the aglycone and enable colonic absorption. The role of the gut microbi...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4425173/ https://www.ncbi.nlm.nih.gov/pubmed/25875120 http://dx.doi.org/10.3390/nu7042788 |
_version_ | 1782370457112018944 |
---|---|
author | Amaretti, Alberto Raimondi, Stefano Leonardi, Alan Quartieri, Andrea Rossi, Maddalena |
author_facet | Amaretti, Alberto Raimondi, Stefano Leonardi, Alan Quartieri, Andrea Rossi, Maddalena |
author_sort | Amaretti, Alberto |
collection | PubMed |
description | Flavonols and flavanones are polyphenols exerting many healthy biological activities. They are often glycosylated by rutinose, which hampers absorption in the small intestine. Therefore they require the gut microbiota to release the aglycone and enable colonic absorption. The role of the gut microbiota and bifidobacteria in the release of the aglycones from two major rutinosides, hesperidin and rutin, was investigated. In bioconversion experiments, the microbiota removed rutinose from both rutin and hesperidin, even though complete hydrolysis was not obtained. To investigate whether bifidobacteria can participate to the hydrolysis of rutinosides, 33 strains were screened. Rutin was resistant to hydrolysis by all the strains. Among six tested species, mostly Bifidobacterium catenulatum and Bifidobacterium pseudocatenultum were able to hydrolyze hesperidin, by means of a cell-associated activity. This result is in agreement with the presence of a putative α-l-rhamnosidase in the genome of B. pseudocatenulatum, while most of the available genome sequences of bifidobacteria aside from this species do not bear this sequence. Even though B. pseudocatenulatum may contribute to the release of the aglycone from certain rutinose-conjugated polyphenols, such as hesperidin, it remains to be clarified whether this species may exert a role in affecting the bioavailability of the rutinoside in vivo. |
format | Online Article Text |
id | pubmed-4425173 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-44251732015-05-11 Hydrolysis of the Rutinose-Conjugates Flavonoids Rutin and Hesperidin by the Gut Microbiota and Bifidobacteria Amaretti, Alberto Raimondi, Stefano Leonardi, Alan Quartieri, Andrea Rossi, Maddalena Nutrients Article Flavonols and flavanones are polyphenols exerting many healthy biological activities. They are often glycosylated by rutinose, which hampers absorption in the small intestine. Therefore they require the gut microbiota to release the aglycone and enable colonic absorption. The role of the gut microbiota and bifidobacteria in the release of the aglycones from two major rutinosides, hesperidin and rutin, was investigated. In bioconversion experiments, the microbiota removed rutinose from both rutin and hesperidin, even though complete hydrolysis was not obtained. To investigate whether bifidobacteria can participate to the hydrolysis of rutinosides, 33 strains were screened. Rutin was resistant to hydrolysis by all the strains. Among six tested species, mostly Bifidobacterium catenulatum and Bifidobacterium pseudocatenultum were able to hydrolyze hesperidin, by means of a cell-associated activity. This result is in agreement with the presence of a putative α-l-rhamnosidase in the genome of B. pseudocatenulatum, while most of the available genome sequences of bifidobacteria aside from this species do not bear this sequence. Even though B. pseudocatenulatum may contribute to the release of the aglycone from certain rutinose-conjugated polyphenols, such as hesperidin, it remains to be clarified whether this species may exert a role in affecting the bioavailability of the rutinoside in vivo. MDPI 2015-04-14 /pmc/articles/PMC4425173/ /pubmed/25875120 http://dx.doi.org/10.3390/nu7042788 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Amaretti, Alberto Raimondi, Stefano Leonardi, Alan Quartieri, Andrea Rossi, Maddalena Hydrolysis of the Rutinose-Conjugates Flavonoids Rutin and Hesperidin by the Gut Microbiota and Bifidobacteria |
title | Hydrolysis of the Rutinose-Conjugates Flavonoids Rutin and Hesperidin by the Gut Microbiota and Bifidobacteria |
title_full | Hydrolysis of the Rutinose-Conjugates Flavonoids Rutin and Hesperidin by the Gut Microbiota and Bifidobacteria |
title_fullStr | Hydrolysis of the Rutinose-Conjugates Flavonoids Rutin and Hesperidin by the Gut Microbiota and Bifidobacteria |
title_full_unstemmed | Hydrolysis of the Rutinose-Conjugates Flavonoids Rutin and Hesperidin by the Gut Microbiota and Bifidobacteria |
title_short | Hydrolysis of the Rutinose-Conjugates Flavonoids Rutin and Hesperidin by the Gut Microbiota and Bifidobacteria |
title_sort | hydrolysis of the rutinose-conjugates flavonoids rutin and hesperidin by the gut microbiota and bifidobacteria |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4425173/ https://www.ncbi.nlm.nih.gov/pubmed/25875120 http://dx.doi.org/10.3390/nu7042788 |
work_keys_str_mv | AT amarettialberto hydrolysisoftherutinoseconjugatesflavonoidsrutinandhesperidinbythegutmicrobiotaandbifidobacteria AT raimondistefano hydrolysisoftherutinoseconjugatesflavonoidsrutinandhesperidinbythegutmicrobiotaandbifidobacteria AT leonardialan hydrolysisoftherutinoseconjugatesflavonoidsrutinandhesperidinbythegutmicrobiotaandbifidobacteria AT quartieriandrea hydrolysisoftherutinoseconjugatesflavonoidsrutinandhesperidinbythegutmicrobiotaandbifidobacteria AT rossimaddalena hydrolysisoftherutinoseconjugatesflavonoidsrutinandhesperidinbythegutmicrobiotaandbifidobacteria |