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Boswellia serrata Preserves Intestinal Epithelial Barrier from Oxidative and Inflammatory Damage
Aminosalicylates, corticosteroids and immunosuppressants are currently the therapeutic choices in inflammatory bowel diseases (IBD), however, with limited remission and often serious side effects. Meanwhile complementary and alternative medicine (CAM) use is increasing, particularly herbal medicine....
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4425476/ https://www.ncbi.nlm.nih.gov/pubmed/25955295 http://dx.doi.org/10.1371/journal.pone.0125375 |
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author | Catanzaro, Daniela Rancan, Serena Orso, Genny Dall’Acqua, Stefano Brun, Paola Giron, Maria Cecilia Carrara, Maria Castagliuolo, Ignazio Ragazzi, Eugenio Caparrotta, Laura Montopoli, Monica |
author_facet | Catanzaro, Daniela Rancan, Serena Orso, Genny Dall’Acqua, Stefano Brun, Paola Giron, Maria Cecilia Carrara, Maria Castagliuolo, Ignazio Ragazzi, Eugenio Caparrotta, Laura Montopoli, Monica |
author_sort | Catanzaro, Daniela |
collection | PubMed |
description | Aminosalicylates, corticosteroids and immunosuppressants are currently the therapeutic choices in inflammatory bowel diseases (IBD), however, with limited remission and often serious side effects. Meanwhile complementary and alternative medicine (CAM) use is increasing, particularly herbal medicine. Boswellia serrata is a traditional Ayurvedic remedy with anti-inflammatory properties, of interest for its usefulness in IBDs. The mechanism of this pharmacological potential of Boswellia serrata was investigated in colonic epithelial cell monolayers exposed to H(2)O(2) or INF-γ+TNF-α, chosen as in vitro experimental model of intestinal inflammation. The barrier function was evaluated by the transepithelial electrical resistance (TEER) and paracellular permeability assay, and by the tight junction proteins (zonula occludens-1, ZO-1 and occludin) immunofluorescence. The expression of phosphorylated NF-κB and reactive oxygen species (ROS) generation were determined by immunoblot and cytofluorimetric assay, respectively. Boswellia serrata oleo-gum extract (BSE) and its pure derivative acetyl-11-keto-β-boswellic acid (AKBA), were tested at 0.1-10 μg/ml and 0.027μg/ml, respectively. BSE and AKBA safety was demonstrated by no alteration of intestinal cell viability and barrier function and integrity biomarkers. H(2)O(2) or INF-γ+TNF-α treatment of Caco-2 cell monolayers significantly reduced TEER, increased paracellular permeability and caused the disassembly of tight junction proteins occludin and ZO-1. BSE and AKBA pretreatment significantly prevented functional and morphological alterations and also the NF-κB phosphorylation induced by the inflammatory stimuli. At the same concentrations BSE and AKBA counteracted the increase of ROS caused by H(2)O(2) exposure. Data showed the positive correlation of the antioxidant activity with the mechanism involved in the physiologic maintenance of the integrity and function of the intestinal epithelium. This study elucidates the pharmacological mechanisms mediated by BSE, in protecting intestinal epithelial barrier from inflammatory damage and supports its use as safe adjuvant in patients affected by IBD. |
format | Online Article Text |
id | pubmed-4425476 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-44254762015-05-21 Boswellia serrata Preserves Intestinal Epithelial Barrier from Oxidative and Inflammatory Damage Catanzaro, Daniela Rancan, Serena Orso, Genny Dall’Acqua, Stefano Brun, Paola Giron, Maria Cecilia Carrara, Maria Castagliuolo, Ignazio Ragazzi, Eugenio Caparrotta, Laura Montopoli, Monica PLoS One Research Article Aminosalicylates, corticosteroids and immunosuppressants are currently the therapeutic choices in inflammatory bowel diseases (IBD), however, with limited remission and often serious side effects. Meanwhile complementary and alternative medicine (CAM) use is increasing, particularly herbal medicine. Boswellia serrata is a traditional Ayurvedic remedy with anti-inflammatory properties, of interest for its usefulness in IBDs. The mechanism of this pharmacological potential of Boswellia serrata was investigated in colonic epithelial cell monolayers exposed to H(2)O(2) or INF-γ+TNF-α, chosen as in vitro experimental model of intestinal inflammation. The barrier function was evaluated by the transepithelial electrical resistance (TEER) and paracellular permeability assay, and by the tight junction proteins (zonula occludens-1, ZO-1 and occludin) immunofluorescence. The expression of phosphorylated NF-κB and reactive oxygen species (ROS) generation were determined by immunoblot and cytofluorimetric assay, respectively. Boswellia serrata oleo-gum extract (BSE) and its pure derivative acetyl-11-keto-β-boswellic acid (AKBA), were tested at 0.1-10 μg/ml and 0.027μg/ml, respectively. BSE and AKBA safety was demonstrated by no alteration of intestinal cell viability and barrier function and integrity biomarkers. H(2)O(2) or INF-γ+TNF-α treatment of Caco-2 cell monolayers significantly reduced TEER, increased paracellular permeability and caused the disassembly of tight junction proteins occludin and ZO-1. BSE and AKBA pretreatment significantly prevented functional and morphological alterations and also the NF-κB phosphorylation induced by the inflammatory stimuli. At the same concentrations BSE and AKBA counteracted the increase of ROS caused by H(2)O(2) exposure. Data showed the positive correlation of the antioxidant activity with the mechanism involved in the physiologic maintenance of the integrity and function of the intestinal epithelium. This study elucidates the pharmacological mechanisms mediated by BSE, in protecting intestinal epithelial barrier from inflammatory damage and supports its use as safe adjuvant in patients affected by IBD. Public Library of Science 2015-05-08 /pmc/articles/PMC4425476/ /pubmed/25955295 http://dx.doi.org/10.1371/journal.pone.0125375 Text en © 2015 Catanzaro et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Catanzaro, Daniela Rancan, Serena Orso, Genny Dall’Acqua, Stefano Brun, Paola Giron, Maria Cecilia Carrara, Maria Castagliuolo, Ignazio Ragazzi, Eugenio Caparrotta, Laura Montopoli, Monica Boswellia serrata Preserves Intestinal Epithelial Barrier from Oxidative and Inflammatory Damage |
title |
Boswellia serrata Preserves Intestinal Epithelial Barrier from Oxidative and Inflammatory Damage |
title_full |
Boswellia serrata Preserves Intestinal Epithelial Barrier from Oxidative and Inflammatory Damage |
title_fullStr |
Boswellia serrata Preserves Intestinal Epithelial Barrier from Oxidative and Inflammatory Damage |
title_full_unstemmed |
Boswellia serrata Preserves Intestinal Epithelial Barrier from Oxidative and Inflammatory Damage |
title_short |
Boswellia serrata Preserves Intestinal Epithelial Barrier from Oxidative and Inflammatory Damage |
title_sort | boswellia serrata preserves intestinal epithelial barrier from oxidative and inflammatory damage |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4425476/ https://www.ncbi.nlm.nih.gov/pubmed/25955295 http://dx.doi.org/10.1371/journal.pone.0125375 |
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