Cargando…

Gut Microbial Dysbiosis May Predict Diarrhea and Fatigue in Patients Undergoing Pelvic Cancer Radiotherapy: A Pilot Study

Fatigue and diarrhea are the most frequent adverse effects of pelvic radiotherapy, while their etiologies are largely unknown. The aim of this study is to investigate the correlations between fatigue, diarrhea, and alterations in gut microbiota induced by pelvic radiotherapy. During the 5-week treat...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Aiping, Ling, Zongxin, Yang, Zhixiang, Kiela, Pawel R., Wang, Tao, Wang, Cheng, Cao, Le, Geng, Fang, Shen, Mingqiang, Ran, Xinze, Su, Yongping, Cheng, Tianmin, Wang, Junping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4425680/
https://www.ncbi.nlm.nih.gov/pubmed/25955845
http://dx.doi.org/10.1371/journal.pone.0126312
_version_ 1782370519292575744
author Wang, Aiping
Ling, Zongxin
Yang, Zhixiang
Kiela, Pawel R.
Wang, Tao
Wang, Cheng
Cao, Le
Geng, Fang
Shen, Mingqiang
Ran, Xinze
Su, Yongping
Cheng, Tianmin
Wang, Junping
author_facet Wang, Aiping
Ling, Zongxin
Yang, Zhixiang
Kiela, Pawel R.
Wang, Tao
Wang, Cheng
Cao, Le
Geng, Fang
Shen, Mingqiang
Ran, Xinze
Su, Yongping
Cheng, Tianmin
Wang, Junping
author_sort Wang, Aiping
collection PubMed
description Fatigue and diarrhea are the most frequent adverse effects of pelvic radiotherapy, while their etiologies are largely unknown. The aim of this study is to investigate the correlations between fatigue, diarrhea, and alterations in gut microbiota induced by pelvic radiotherapy. During the 5-week treatment of pelvic radiotherapy in 11 cancer patients, the general fatigue score significantly increased and was more prominent in the patients with diarrhea. The fatigue score was closely correlated with the decrease of serum citrulline (an indicator of the functional enterocyte mass) and the increases of systemic inflammatory proteins, including haptoglobin, orosomuoid, α(1)-antitrypsin and TNF-α. Serum level of lipopolysaccharide (LPS) was also elevated, especially in the patients with diarrhea indicating epithelial barrier breach and endotoxemia. Pyrosequencing analysis of 16S rRNA gene revealed that microbial diversity, richness, and the Firmicutes/Bacteroidetes ratio were significantly altered prior to radiotherapy in patients who later developed diarrhea. Pelvic radiotherapy induced further changes in fecal microbial ecology, some of which were specific to the patients with or without diarrhea. Our results indicate that gut microbial dysbiosis prior to radiation therapy may be exploited to predict development of diarrhea and to guide preventive treatment options. Radiation-induced dysbiosis may contribute to pelvic radiation disease, including mucositis, diarrhea, systemic inflammatory response, and pelvic radiotherapy-associated fatigue in cancer patients.
format Online
Article
Text
id pubmed-4425680
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-44256802015-05-21 Gut Microbial Dysbiosis May Predict Diarrhea and Fatigue in Patients Undergoing Pelvic Cancer Radiotherapy: A Pilot Study Wang, Aiping Ling, Zongxin Yang, Zhixiang Kiela, Pawel R. Wang, Tao Wang, Cheng Cao, Le Geng, Fang Shen, Mingqiang Ran, Xinze Su, Yongping Cheng, Tianmin Wang, Junping PLoS One Research Article Fatigue and diarrhea are the most frequent adverse effects of pelvic radiotherapy, while their etiologies are largely unknown. The aim of this study is to investigate the correlations between fatigue, diarrhea, and alterations in gut microbiota induced by pelvic radiotherapy. During the 5-week treatment of pelvic radiotherapy in 11 cancer patients, the general fatigue score significantly increased and was more prominent in the patients with diarrhea. The fatigue score was closely correlated with the decrease of serum citrulline (an indicator of the functional enterocyte mass) and the increases of systemic inflammatory proteins, including haptoglobin, orosomuoid, α(1)-antitrypsin and TNF-α. Serum level of lipopolysaccharide (LPS) was also elevated, especially in the patients with diarrhea indicating epithelial barrier breach and endotoxemia. Pyrosequencing analysis of 16S rRNA gene revealed that microbial diversity, richness, and the Firmicutes/Bacteroidetes ratio were significantly altered prior to radiotherapy in patients who later developed diarrhea. Pelvic radiotherapy induced further changes in fecal microbial ecology, some of which were specific to the patients with or without diarrhea. Our results indicate that gut microbial dysbiosis prior to radiation therapy may be exploited to predict development of diarrhea and to guide preventive treatment options. Radiation-induced dysbiosis may contribute to pelvic radiation disease, including mucositis, diarrhea, systemic inflammatory response, and pelvic radiotherapy-associated fatigue in cancer patients. Public Library of Science 2015-05-08 /pmc/articles/PMC4425680/ /pubmed/25955845 http://dx.doi.org/10.1371/journal.pone.0126312 Text en © 2015 Wang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Wang, Aiping
Ling, Zongxin
Yang, Zhixiang
Kiela, Pawel R.
Wang, Tao
Wang, Cheng
Cao, Le
Geng, Fang
Shen, Mingqiang
Ran, Xinze
Su, Yongping
Cheng, Tianmin
Wang, Junping
Gut Microbial Dysbiosis May Predict Diarrhea and Fatigue in Patients Undergoing Pelvic Cancer Radiotherapy: A Pilot Study
title Gut Microbial Dysbiosis May Predict Diarrhea and Fatigue in Patients Undergoing Pelvic Cancer Radiotherapy: A Pilot Study
title_full Gut Microbial Dysbiosis May Predict Diarrhea and Fatigue in Patients Undergoing Pelvic Cancer Radiotherapy: A Pilot Study
title_fullStr Gut Microbial Dysbiosis May Predict Diarrhea and Fatigue in Patients Undergoing Pelvic Cancer Radiotherapy: A Pilot Study
title_full_unstemmed Gut Microbial Dysbiosis May Predict Diarrhea and Fatigue in Patients Undergoing Pelvic Cancer Radiotherapy: A Pilot Study
title_short Gut Microbial Dysbiosis May Predict Diarrhea and Fatigue in Patients Undergoing Pelvic Cancer Radiotherapy: A Pilot Study
title_sort gut microbial dysbiosis may predict diarrhea and fatigue in patients undergoing pelvic cancer radiotherapy: a pilot study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4425680/
https://www.ncbi.nlm.nih.gov/pubmed/25955845
http://dx.doi.org/10.1371/journal.pone.0126312
work_keys_str_mv AT wangaiping gutmicrobialdysbiosismaypredictdiarrheaandfatigueinpatientsundergoingpelviccancerradiotherapyapilotstudy
AT lingzongxin gutmicrobialdysbiosismaypredictdiarrheaandfatigueinpatientsundergoingpelviccancerradiotherapyapilotstudy
AT yangzhixiang gutmicrobialdysbiosismaypredictdiarrheaandfatigueinpatientsundergoingpelviccancerradiotherapyapilotstudy
AT kielapawelr gutmicrobialdysbiosismaypredictdiarrheaandfatigueinpatientsundergoingpelviccancerradiotherapyapilotstudy
AT wangtao gutmicrobialdysbiosismaypredictdiarrheaandfatigueinpatientsundergoingpelviccancerradiotherapyapilotstudy
AT wangcheng gutmicrobialdysbiosismaypredictdiarrheaandfatigueinpatientsundergoingpelviccancerradiotherapyapilotstudy
AT caole gutmicrobialdysbiosismaypredictdiarrheaandfatigueinpatientsundergoingpelviccancerradiotherapyapilotstudy
AT gengfang gutmicrobialdysbiosismaypredictdiarrheaandfatigueinpatientsundergoingpelviccancerradiotherapyapilotstudy
AT shenmingqiang gutmicrobialdysbiosismaypredictdiarrheaandfatigueinpatientsundergoingpelviccancerradiotherapyapilotstudy
AT ranxinze gutmicrobialdysbiosismaypredictdiarrheaandfatigueinpatientsundergoingpelviccancerradiotherapyapilotstudy
AT suyongping gutmicrobialdysbiosismaypredictdiarrheaandfatigueinpatientsundergoingpelviccancerradiotherapyapilotstudy
AT chengtianmin gutmicrobialdysbiosismaypredictdiarrheaandfatigueinpatientsundergoingpelviccancerradiotherapyapilotstudy
AT wangjunping gutmicrobialdysbiosismaypredictdiarrheaandfatigueinpatientsundergoingpelviccancerradiotherapyapilotstudy