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A Multipurpose, High-Throughput Single-Nucleotide Polymorphism Chip for the Dengue and Yellow Fever Mosquito, Aedes aegypti

The dengue and yellow fever mosquito, Aedes aegypti, contributes significantly to global disease burden. Genetic study of Aedes aegypti is essential to understanding its evolutionary history, competence as a disease vector, and the effects and efficacy of vector control methods. The prevalence of re...

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Autores principales: Evans, Benjamin R., Gloria-Soria, Andrea, Hou, Lin, McBride, Carolyn, Bonizzoni, Mariangela, Zhao, Hongyu, Powell, Jeffrey R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4426360/
https://www.ncbi.nlm.nih.gov/pubmed/25721127
http://dx.doi.org/10.1534/g3.114.016196
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author Evans, Benjamin R.
Gloria-Soria, Andrea
Hou, Lin
McBride, Carolyn
Bonizzoni, Mariangela
Zhao, Hongyu
Powell, Jeffrey R.
author_facet Evans, Benjamin R.
Gloria-Soria, Andrea
Hou, Lin
McBride, Carolyn
Bonizzoni, Mariangela
Zhao, Hongyu
Powell, Jeffrey R.
author_sort Evans, Benjamin R.
collection PubMed
description The dengue and yellow fever mosquito, Aedes aegypti, contributes significantly to global disease burden. Genetic study of Aedes aegypti is essential to understanding its evolutionary history, competence as a disease vector, and the effects and efficacy of vector control methods. The prevalence of repeats and transposable elements in the Aedes aegypti genome complicates marker development and makes genome-wide genetic study challenging. To overcome these challenges, we developed a high-throughput genotyping chip, Axiom_aegypti1. This chip screens for 50,000 single-nucleotide polymorphisms present in Aedes aegypti populations from around the world. The array currently used genotypes 96 samples simultaneously. To ensure that these markers satisfy assumptions commonly made in many genetic analyses, we tested for Mendelian inheritance and linkage disequilibrium in laboratory crosses and a wild population, respectively. We have validated more than 25,000 of these markers to date, and expect this number to increase with more sampling. We also present evidence of the chip’s efficacy in distinguishing populations throughout the world. The markers on this chip are ideal for applications ranging from population genetics to genome-wide association studies. This tool makes rapid, cost-effective, and comparable genotype data attainable to diverse sets of Aedes aegypti researchers, from those interested in potential range shifts due to climate change to those characterizing the genetic underpinnings of its competence to transmit disease.
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spelling pubmed-44263602015-05-13 A Multipurpose, High-Throughput Single-Nucleotide Polymorphism Chip for the Dengue and Yellow Fever Mosquito, Aedes aegypti Evans, Benjamin R. Gloria-Soria, Andrea Hou, Lin McBride, Carolyn Bonizzoni, Mariangela Zhao, Hongyu Powell, Jeffrey R. G3 (Bethesda) Investigations The dengue and yellow fever mosquito, Aedes aegypti, contributes significantly to global disease burden. Genetic study of Aedes aegypti is essential to understanding its evolutionary history, competence as a disease vector, and the effects and efficacy of vector control methods. The prevalence of repeats and transposable elements in the Aedes aegypti genome complicates marker development and makes genome-wide genetic study challenging. To overcome these challenges, we developed a high-throughput genotyping chip, Axiom_aegypti1. This chip screens for 50,000 single-nucleotide polymorphisms present in Aedes aegypti populations from around the world. The array currently used genotypes 96 samples simultaneously. To ensure that these markers satisfy assumptions commonly made in many genetic analyses, we tested for Mendelian inheritance and linkage disequilibrium in laboratory crosses and a wild population, respectively. We have validated more than 25,000 of these markers to date, and expect this number to increase with more sampling. We also present evidence of the chip’s efficacy in distinguishing populations throughout the world. The markers on this chip are ideal for applications ranging from population genetics to genome-wide association studies. This tool makes rapid, cost-effective, and comparable genotype data attainable to diverse sets of Aedes aegypti researchers, from those interested in potential range shifts due to climate change to those characterizing the genetic underpinnings of its competence to transmit disease. Genetics Society of America 2015-02-26 /pmc/articles/PMC4426360/ /pubmed/25721127 http://dx.doi.org/10.1534/g3.114.016196 Text en Copyright © 2015 Evans et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Investigations
Evans, Benjamin R.
Gloria-Soria, Andrea
Hou, Lin
McBride, Carolyn
Bonizzoni, Mariangela
Zhao, Hongyu
Powell, Jeffrey R.
A Multipurpose, High-Throughput Single-Nucleotide Polymorphism Chip for the Dengue and Yellow Fever Mosquito, Aedes aegypti
title A Multipurpose, High-Throughput Single-Nucleotide Polymorphism Chip for the Dengue and Yellow Fever Mosquito, Aedes aegypti
title_full A Multipurpose, High-Throughput Single-Nucleotide Polymorphism Chip for the Dengue and Yellow Fever Mosquito, Aedes aegypti
title_fullStr A Multipurpose, High-Throughput Single-Nucleotide Polymorphism Chip for the Dengue and Yellow Fever Mosquito, Aedes aegypti
title_full_unstemmed A Multipurpose, High-Throughput Single-Nucleotide Polymorphism Chip for the Dengue and Yellow Fever Mosquito, Aedes aegypti
title_short A Multipurpose, High-Throughput Single-Nucleotide Polymorphism Chip for the Dengue and Yellow Fever Mosquito, Aedes aegypti
title_sort multipurpose, high-throughput single-nucleotide polymorphism chip for the dengue and yellow fever mosquito, aedes aegypti
topic Investigations
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4426360/
https://www.ncbi.nlm.nih.gov/pubmed/25721127
http://dx.doi.org/10.1534/g3.114.016196
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