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A Systematic Mutational Analysis of a Histone H3 Residue in Budding Yeast Provides Insights into Chromatin Dynamics

In previous work using the Saccharomyces cerevisiae model system, a mutant version of histone H3—H3-L61W—was found to confer a variety of abnormal growth phenotypes and defects in specific aspects of the transcription process, including a pronounced alteration in the distribution pattern of the tran...

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Autores principales: Johnson, Paige, Mitchell, Virginia, McClure, Kelsi, Kellems, Martha, Marshall, Sarah, Allison, Mary K., Lindley, Harrison, Nguyen, Hoai-Trang T., Tackett, Jessalyn E., Duina, Andrea A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4426362/
https://www.ncbi.nlm.nih.gov/pubmed/25711831
http://dx.doi.org/10.1534/g3.115.017376
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author Johnson, Paige
Mitchell, Virginia
McClure, Kelsi
Kellems, Martha
Marshall, Sarah
Allison, Mary K.
Lindley, Harrison
Nguyen, Hoai-Trang T.
Tackett, Jessalyn E.
Duina, Andrea A.
author_facet Johnson, Paige
Mitchell, Virginia
McClure, Kelsi
Kellems, Martha
Marshall, Sarah
Allison, Mary K.
Lindley, Harrison
Nguyen, Hoai-Trang T.
Tackett, Jessalyn E.
Duina, Andrea A.
author_sort Johnson, Paige
collection PubMed
description In previous work using the Saccharomyces cerevisiae model system, a mutant version of histone H3—H3-L61W—was found to confer a variety of abnormal growth phenotypes and defects in specific aspects of the transcription process, including a pronounced alteration in the distribution pattern of the transcription elongation factor Spt16 across transcribed genes and promotion of cryptic transcription initiation within the FLO8 gene. To gain insights into the contribution of the H3-L61 residue to chromatin function, we have generated yeast strains expressing versions of histone H3 harboring all possible natural amino acid substitutions at position 61 (H3-L61X mutants) and tested them in a series of assays. We found that whereas 16 of the 19 H3-L61X mutants support viability when expressed as the sole source of histone H3 in cells, all 19 confer abnormal phenotypes ranging from very mild to severe, a finding that might in part explain the high degree of conservation of the H3-L61 residue among eukaryotes. An examination of the strength of the defects conferred by each H3-L61X mutant and the nature of the corresponding substituted residue provides insights into structural features of the nucleosome required for proper Spt16−gene interactions and for prevention of cryptic transcription initiation events. Finally, we provide evidence that the defects imparted by H3-L61X mutants on Spt16−gene interactions and on repression of intragenic transcription initiation are mechanistically related to each other.
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spelling pubmed-44263622015-05-14 A Systematic Mutational Analysis of a Histone H3 Residue in Budding Yeast Provides Insights into Chromatin Dynamics Johnson, Paige Mitchell, Virginia McClure, Kelsi Kellems, Martha Marshall, Sarah Allison, Mary K. Lindley, Harrison Nguyen, Hoai-Trang T. Tackett, Jessalyn E. Duina, Andrea A. G3 (Bethesda) Investigations In previous work using the Saccharomyces cerevisiae model system, a mutant version of histone H3—H3-L61W—was found to confer a variety of abnormal growth phenotypes and defects in specific aspects of the transcription process, including a pronounced alteration in the distribution pattern of the transcription elongation factor Spt16 across transcribed genes and promotion of cryptic transcription initiation within the FLO8 gene. To gain insights into the contribution of the H3-L61 residue to chromatin function, we have generated yeast strains expressing versions of histone H3 harboring all possible natural amino acid substitutions at position 61 (H3-L61X mutants) and tested them in a series of assays. We found that whereas 16 of the 19 H3-L61X mutants support viability when expressed as the sole source of histone H3 in cells, all 19 confer abnormal phenotypes ranging from very mild to severe, a finding that might in part explain the high degree of conservation of the H3-L61 residue among eukaryotes. An examination of the strength of the defects conferred by each H3-L61X mutant and the nature of the corresponding substituted residue provides insights into structural features of the nucleosome required for proper Spt16−gene interactions and for prevention of cryptic transcription initiation events. Finally, we provide evidence that the defects imparted by H3-L61X mutants on Spt16−gene interactions and on repression of intragenic transcription initiation are mechanistically related to each other. Genetics Society of America 2015-02-23 /pmc/articles/PMC4426362/ /pubmed/25711831 http://dx.doi.org/10.1534/g3.115.017376 Text en Copyright © 2015 Johnson et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Investigations
Johnson, Paige
Mitchell, Virginia
McClure, Kelsi
Kellems, Martha
Marshall, Sarah
Allison, Mary K.
Lindley, Harrison
Nguyen, Hoai-Trang T.
Tackett, Jessalyn E.
Duina, Andrea A.
A Systematic Mutational Analysis of a Histone H3 Residue in Budding Yeast Provides Insights into Chromatin Dynamics
title A Systematic Mutational Analysis of a Histone H3 Residue in Budding Yeast Provides Insights into Chromatin Dynamics
title_full A Systematic Mutational Analysis of a Histone H3 Residue in Budding Yeast Provides Insights into Chromatin Dynamics
title_fullStr A Systematic Mutational Analysis of a Histone H3 Residue in Budding Yeast Provides Insights into Chromatin Dynamics
title_full_unstemmed A Systematic Mutational Analysis of a Histone H3 Residue in Budding Yeast Provides Insights into Chromatin Dynamics
title_short A Systematic Mutational Analysis of a Histone H3 Residue in Budding Yeast Provides Insights into Chromatin Dynamics
title_sort systematic mutational analysis of a histone h3 residue in budding yeast provides insights into chromatin dynamics
topic Investigations
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4426362/
https://www.ncbi.nlm.nih.gov/pubmed/25711831
http://dx.doi.org/10.1534/g3.115.017376
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