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Bone Marrow Recovery by Morphometry during Induction Chemotherapy for Acute Lymphoblastic Leukemia in Children

Bone marrow architecture is grossly distorted at the diagnosis of ALL and details of the morphological changes that accompany response to Induction chemotherapy have not been reported before. While marrow aspirates are widely used to assess initial response to ALL therapy and provide some indication...

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Autores principales: Nguyen, Tuong-Vi, Melville, Anna, Nath, Shriram, Story, Colin, Howell, Stuart, Sutton, Rosemary, Zannettino, Andrew, Revesz, Tamas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4427405/
https://www.ncbi.nlm.nih.gov/pubmed/25962143
http://dx.doi.org/10.1371/journal.pone.0126233
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author Nguyen, Tuong-Vi
Melville, Anna
Nath, Shriram
Story, Colin
Howell, Stuart
Sutton, Rosemary
Zannettino, Andrew
Revesz, Tamas
author_facet Nguyen, Tuong-Vi
Melville, Anna
Nath, Shriram
Story, Colin
Howell, Stuart
Sutton, Rosemary
Zannettino, Andrew
Revesz, Tamas
author_sort Nguyen, Tuong-Vi
collection PubMed
description Bone marrow architecture is grossly distorted at the diagnosis of ALL and details of the morphological changes that accompany response to Induction chemotherapy have not been reported before. While marrow aspirates are widely used to assess initial response to ALL therapy and provide some indications, we have enumerated marrow components using morphometric analysis of trephine samples with the aim of achieving a greater understanding of changes in bone marrow niches. Morphometric analyses were carried out in the bone marrow trephine samples of 44 children with ALL, using a NanoZoomer HT digital scanner. Diagnostic samples were compared to those of 32 control patients with solid tumors but without marrow involvement. Samples from patients with ALL had significantly increased fibrosis and the area occupied by bony trabeculae was lower than in controls. Cellularity was higher in ALL samples due to leukemic infiltration while the percentage of normal elements such as megakaryocytes, adipocytes, osteoblasts and osteoclasts were all significantly lower. During the course of Induction therapy, there was a decrease in the cellularity of ALL samples at day 15 of therapy with a further decrease at the end of Induction and an increase in the area occupied by adipocytes and the width of sinusoids. Reticulin fibrosis decreased throughout Induction. Megakaryocytes increased, osteoblasts and osteoclasts remained unchanged. No correlation was found between clinical presentation, early response to treatment and morphological changes. Our results provide a morphological background to further studies of bone marrow stroma in ALL.
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spelling pubmed-44274052015-05-21 Bone Marrow Recovery by Morphometry during Induction Chemotherapy for Acute Lymphoblastic Leukemia in Children Nguyen, Tuong-Vi Melville, Anna Nath, Shriram Story, Colin Howell, Stuart Sutton, Rosemary Zannettino, Andrew Revesz, Tamas PLoS One Research Article Bone marrow architecture is grossly distorted at the diagnosis of ALL and details of the morphological changes that accompany response to Induction chemotherapy have not been reported before. While marrow aspirates are widely used to assess initial response to ALL therapy and provide some indications, we have enumerated marrow components using morphometric analysis of trephine samples with the aim of achieving a greater understanding of changes in bone marrow niches. Morphometric analyses were carried out in the bone marrow trephine samples of 44 children with ALL, using a NanoZoomer HT digital scanner. Diagnostic samples were compared to those of 32 control patients with solid tumors but without marrow involvement. Samples from patients with ALL had significantly increased fibrosis and the area occupied by bony trabeculae was lower than in controls. Cellularity was higher in ALL samples due to leukemic infiltration while the percentage of normal elements such as megakaryocytes, adipocytes, osteoblasts and osteoclasts were all significantly lower. During the course of Induction therapy, there was a decrease in the cellularity of ALL samples at day 15 of therapy with a further decrease at the end of Induction and an increase in the area occupied by adipocytes and the width of sinusoids. Reticulin fibrosis decreased throughout Induction. Megakaryocytes increased, osteoblasts and osteoclasts remained unchanged. No correlation was found between clinical presentation, early response to treatment and morphological changes. Our results provide a morphological background to further studies of bone marrow stroma in ALL. Public Library of Science 2015-05-11 /pmc/articles/PMC4427405/ /pubmed/25962143 http://dx.doi.org/10.1371/journal.pone.0126233 Text en © 2015 Nguyen et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Nguyen, Tuong-Vi
Melville, Anna
Nath, Shriram
Story, Colin
Howell, Stuart
Sutton, Rosemary
Zannettino, Andrew
Revesz, Tamas
Bone Marrow Recovery by Morphometry during Induction Chemotherapy for Acute Lymphoblastic Leukemia in Children
title Bone Marrow Recovery by Morphometry during Induction Chemotherapy for Acute Lymphoblastic Leukemia in Children
title_full Bone Marrow Recovery by Morphometry during Induction Chemotherapy for Acute Lymphoblastic Leukemia in Children
title_fullStr Bone Marrow Recovery by Morphometry during Induction Chemotherapy for Acute Lymphoblastic Leukemia in Children
title_full_unstemmed Bone Marrow Recovery by Morphometry during Induction Chemotherapy for Acute Lymphoblastic Leukemia in Children
title_short Bone Marrow Recovery by Morphometry during Induction Chemotherapy for Acute Lymphoblastic Leukemia in Children
title_sort bone marrow recovery by morphometry during induction chemotherapy for acute lymphoblastic leukemia in children
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4427405/
https://www.ncbi.nlm.nih.gov/pubmed/25962143
http://dx.doi.org/10.1371/journal.pone.0126233
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