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Human Bone Marrow-Derived Mesenchymal Stromal Cells Differentially Inhibit Cytokine Production by Peripheral Blood Monocytes Subpopulations and Myeloid Dendritic Cells

The immunosuppressive properties of mesenchymal stromal/stem cells (MSC) rendered them an attractive therapeutic approach for immune disorders and an increasing body of evidence demonstrated their clinical value. However, the influence of MSC on the function of specific immune cell populations, name...

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Autores principales: Laranjeira, Paula, Gomes, Joana, Pedreiro, Susana, Pedrosa, Monia, Martinho, Antonio, Antunes, Brigida, Ribeiro, Tania, Santos, Francisco, Domingues, Rosario, Abecasis, Manuel, Trindade, Helder, Paiva, Artur
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4427776/
https://www.ncbi.nlm.nih.gov/pubmed/26060498
http://dx.doi.org/10.1155/2015/819084
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author Laranjeira, Paula
Gomes, Joana
Pedreiro, Susana
Pedrosa, Monia
Martinho, Antonio
Antunes, Brigida
Ribeiro, Tania
Santos, Francisco
Domingues, Rosario
Abecasis, Manuel
Trindade, Helder
Paiva, Artur
author_facet Laranjeira, Paula
Gomes, Joana
Pedreiro, Susana
Pedrosa, Monia
Martinho, Antonio
Antunes, Brigida
Ribeiro, Tania
Santos, Francisco
Domingues, Rosario
Abecasis, Manuel
Trindade, Helder
Paiva, Artur
author_sort Laranjeira, Paula
collection PubMed
description The immunosuppressive properties of mesenchymal stromal/stem cells (MSC) rendered them an attractive therapeutic approach for immune disorders and an increasing body of evidence demonstrated their clinical value. However, the influence of MSC on the function of specific immune cell populations, namely, monocyte subpopulations, is not well elucidated. Here, we investigated the influence of human bone marrow MSC on the cytokine and chemokine expression by peripheral blood classical, intermediate and nonclassical monocytes, and myeloid dendritic cells (mDC), stimulated with lipopolysaccharide plus interferon (IFN)γ. We found that MSC effectively inhibit tumor necrosis factor- (TNF-) α and macrophage inflammatory protein- (MIP-) 1β protein expression in monocytes and mDC, without suppressing CCR7 and CD83 protein expression. Interestingly, mDC exhibited the highest degree of inhibition, for both TNF-α and MIP-1β, whereas the reduction of TNF-α expression was less marked for nonclassical monocytes. Similarly, MSC decreased mRNA levels of interleukin- (IL-) 1β and IL-6 in classical monocytes, CCL3, CCL5, CXCL9, and CXCL10 in classical and nonclassical monocytes, and IL-1β and CXCL10 in mDC. MSC do not impair the expression of maturation markers in monocytes and mDC under our experimental conditions; nevertheless, they hamper the proinflammatory function of monocytes and mDC, which may impede the development of inflammatory immune responses.
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spelling pubmed-44277762015-06-09 Human Bone Marrow-Derived Mesenchymal Stromal Cells Differentially Inhibit Cytokine Production by Peripheral Blood Monocytes Subpopulations and Myeloid Dendritic Cells Laranjeira, Paula Gomes, Joana Pedreiro, Susana Pedrosa, Monia Martinho, Antonio Antunes, Brigida Ribeiro, Tania Santos, Francisco Domingues, Rosario Abecasis, Manuel Trindade, Helder Paiva, Artur Stem Cells Int Research Article The immunosuppressive properties of mesenchymal stromal/stem cells (MSC) rendered them an attractive therapeutic approach for immune disorders and an increasing body of evidence demonstrated their clinical value. However, the influence of MSC on the function of specific immune cell populations, namely, monocyte subpopulations, is not well elucidated. Here, we investigated the influence of human bone marrow MSC on the cytokine and chemokine expression by peripheral blood classical, intermediate and nonclassical monocytes, and myeloid dendritic cells (mDC), stimulated with lipopolysaccharide plus interferon (IFN)γ. We found that MSC effectively inhibit tumor necrosis factor- (TNF-) α and macrophage inflammatory protein- (MIP-) 1β protein expression in monocytes and mDC, without suppressing CCR7 and CD83 protein expression. Interestingly, mDC exhibited the highest degree of inhibition, for both TNF-α and MIP-1β, whereas the reduction of TNF-α expression was less marked for nonclassical monocytes. Similarly, MSC decreased mRNA levels of interleukin- (IL-) 1β and IL-6 in classical monocytes, CCL3, CCL5, CXCL9, and CXCL10 in classical and nonclassical monocytes, and IL-1β and CXCL10 in mDC. MSC do not impair the expression of maturation markers in monocytes and mDC under our experimental conditions; nevertheless, they hamper the proinflammatory function of monocytes and mDC, which may impede the development of inflammatory immune responses. Hindawi Publishing Corporation 2015 2015-04-28 /pmc/articles/PMC4427776/ /pubmed/26060498 http://dx.doi.org/10.1155/2015/819084 Text en Copyright © 2015 Paula Laranjeira et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Laranjeira, Paula
Gomes, Joana
Pedreiro, Susana
Pedrosa, Monia
Martinho, Antonio
Antunes, Brigida
Ribeiro, Tania
Santos, Francisco
Domingues, Rosario
Abecasis, Manuel
Trindade, Helder
Paiva, Artur
Human Bone Marrow-Derived Mesenchymal Stromal Cells Differentially Inhibit Cytokine Production by Peripheral Blood Monocytes Subpopulations and Myeloid Dendritic Cells
title Human Bone Marrow-Derived Mesenchymal Stromal Cells Differentially Inhibit Cytokine Production by Peripheral Blood Monocytes Subpopulations and Myeloid Dendritic Cells
title_full Human Bone Marrow-Derived Mesenchymal Stromal Cells Differentially Inhibit Cytokine Production by Peripheral Blood Monocytes Subpopulations and Myeloid Dendritic Cells
title_fullStr Human Bone Marrow-Derived Mesenchymal Stromal Cells Differentially Inhibit Cytokine Production by Peripheral Blood Monocytes Subpopulations and Myeloid Dendritic Cells
title_full_unstemmed Human Bone Marrow-Derived Mesenchymal Stromal Cells Differentially Inhibit Cytokine Production by Peripheral Blood Monocytes Subpopulations and Myeloid Dendritic Cells
title_short Human Bone Marrow-Derived Mesenchymal Stromal Cells Differentially Inhibit Cytokine Production by Peripheral Blood Monocytes Subpopulations and Myeloid Dendritic Cells
title_sort human bone marrow-derived mesenchymal stromal cells differentially inhibit cytokine production by peripheral blood monocytes subpopulations and myeloid dendritic cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4427776/
https://www.ncbi.nlm.nih.gov/pubmed/26060498
http://dx.doi.org/10.1155/2015/819084
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