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Antimicrobial activity and safety evaluation of Enterococcus faecium KQ 2.6 isolated from peacock feces
BACKGROUND: The objective of this paper was to study antimicrobial activity and safety of Enterococcus faecium KQ 2.6 (E. faecium KQ 2.6) isolated from peacock feces. METHODS: Agar well diffusion method was adopted in antimicrobial activity assay. Disk diffusion test was used to determine the antibi...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4427955/ https://www.ncbi.nlm.nih.gov/pubmed/25962418 http://dx.doi.org/10.1186/s12896-015-0151-y |
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author | Zheng, Wei Zhang, Yu Lu, Hui-Min Li, Dan-Ting Zhang, Zhi-Liang Tang, Zhen-Xing Shi, Lu-E |
author_facet | Zheng, Wei Zhang, Yu Lu, Hui-Min Li, Dan-Ting Zhang, Zhi-Liang Tang, Zhen-Xing Shi, Lu-E |
author_sort | Zheng, Wei |
collection | PubMed |
description | BACKGROUND: The objective of this paper was to study antimicrobial activity and safety of Enterococcus faecium KQ 2.6 (E. faecium KQ 2.6) isolated from peacock feces. METHODS: Agar well diffusion method was adopted in antimicrobial activity assay. Disk diffusion test was used to determine the antibiotic resistance. The identification and virulence potential of E. faecium KQ 2.6 were investigated using PCR amplification. RESULTS: The results indicated that cell free supernatant (CFS) of the strain had the good antimicrobial activity against selected gram-positive and gram-negative bacteria. The biochemical characteristics of antimicrobial substances were investigated. The results indicated that the antimicrobial substances were still active after treatment with catalase and proteinase, respectively. Moreover, the stability of antimicrobial substances did not change after heat treatment at 40, 50, 60, 70 and 80°C for 30 min, respectively. The activity of antimicrobial substances remained stable at 4 and −20°C after long time storage. The antimicrobial activity of CFS was compared with that of the buffer with similar strength and pH. The inhibitory zone of the buffer was apparently smaller than that of CFS, which meant that the acid in CFS was not the only factor that was contributed to antibacterial activity of CFS. The antibiotic resistance and virulence potential were evaluated using disk diffusion test and PCR amplification. The results showed that E. faecium KQ 2.6 did not harbor any tested virulence genes such as gelE, esp, asa1, cylA, efaA and hyl. It was susceptible to most of tested antibiotics except for vancomycin and polymyxin B. CONCLUSION: E. faecium KQ 2.6 may be used as bio-preservative cultures for the production of fermented foods. |
format | Online Article Text |
id | pubmed-4427955 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-44279552015-05-13 Antimicrobial activity and safety evaluation of Enterococcus faecium KQ 2.6 isolated from peacock feces Zheng, Wei Zhang, Yu Lu, Hui-Min Li, Dan-Ting Zhang, Zhi-Liang Tang, Zhen-Xing Shi, Lu-E BMC Biotechnol Research Article BACKGROUND: The objective of this paper was to study antimicrobial activity and safety of Enterococcus faecium KQ 2.6 (E. faecium KQ 2.6) isolated from peacock feces. METHODS: Agar well diffusion method was adopted in antimicrobial activity assay. Disk diffusion test was used to determine the antibiotic resistance. The identification and virulence potential of E. faecium KQ 2.6 were investigated using PCR amplification. RESULTS: The results indicated that cell free supernatant (CFS) of the strain had the good antimicrobial activity against selected gram-positive and gram-negative bacteria. The biochemical characteristics of antimicrobial substances were investigated. The results indicated that the antimicrobial substances were still active after treatment with catalase and proteinase, respectively. Moreover, the stability of antimicrobial substances did not change after heat treatment at 40, 50, 60, 70 and 80°C for 30 min, respectively. The activity of antimicrobial substances remained stable at 4 and −20°C after long time storage. The antimicrobial activity of CFS was compared with that of the buffer with similar strength and pH. The inhibitory zone of the buffer was apparently smaller than that of CFS, which meant that the acid in CFS was not the only factor that was contributed to antibacterial activity of CFS. The antibiotic resistance and virulence potential were evaluated using disk diffusion test and PCR amplification. The results showed that E. faecium KQ 2.6 did not harbor any tested virulence genes such as gelE, esp, asa1, cylA, efaA and hyl. It was susceptible to most of tested antibiotics except for vancomycin and polymyxin B. CONCLUSION: E. faecium KQ 2.6 may be used as bio-preservative cultures for the production of fermented foods. BioMed Central 2015-05-12 /pmc/articles/PMC4427955/ /pubmed/25962418 http://dx.doi.org/10.1186/s12896-015-0151-y Text en © Zheng et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Zheng, Wei Zhang, Yu Lu, Hui-Min Li, Dan-Ting Zhang, Zhi-Liang Tang, Zhen-Xing Shi, Lu-E Antimicrobial activity and safety evaluation of Enterococcus faecium KQ 2.6 isolated from peacock feces |
title | Antimicrobial activity and safety evaluation of Enterococcus faecium KQ 2.6 isolated from peacock feces |
title_full | Antimicrobial activity and safety evaluation of Enterococcus faecium KQ 2.6 isolated from peacock feces |
title_fullStr | Antimicrobial activity and safety evaluation of Enterococcus faecium KQ 2.6 isolated from peacock feces |
title_full_unstemmed | Antimicrobial activity and safety evaluation of Enterococcus faecium KQ 2.6 isolated from peacock feces |
title_short | Antimicrobial activity and safety evaluation of Enterococcus faecium KQ 2.6 isolated from peacock feces |
title_sort | antimicrobial activity and safety evaluation of enterococcus faecium kq 2.6 isolated from peacock feces |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4427955/ https://www.ncbi.nlm.nih.gov/pubmed/25962418 http://dx.doi.org/10.1186/s12896-015-0151-y |
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