Cargando…

Gene and protein expression of glucose transporter 1 and glucose transporter 3 in human laryngeal cancer—the relationship with regulatory hypoxia-inducible factor-1α expression, tumor invasiveness, and patient prognosis

Increased glucose uptake mediated by glucose transporters and reliance on glycolysis are common features of malignant cells. Hypoxia-inducible factor-1α supports the adaptation of hypoxic cells by inducing genes related to glucose metabolism. The contribution of glucose transporter (GLUT) and hypoxi...

Descripción completa

Detalles Bibliográficos
Autores principales: Starska, Katarzyna, Forma, Ewa, Jóźwiak, Paweł, Bryś, Magdalena, Lewy-Trenda, Iwona, Brzezińska-Błaszczyk, Ewa, Krześlak, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4428538/
https://www.ncbi.nlm.nih.gov/pubmed/25412955
http://dx.doi.org/10.1007/s13277-014-2838-4
_version_ 1782370912510672896
author Starska, Katarzyna
Forma, Ewa
Jóźwiak, Paweł
Bryś, Magdalena
Lewy-Trenda, Iwona
Brzezińska-Błaszczyk, Ewa
Krześlak, Anna
author_facet Starska, Katarzyna
Forma, Ewa
Jóźwiak, Paweł
Bryś, Magdalena
Lewy-Trenda, Iwona
Brzezińska-Błaszczyk, Ewa
Krześlak, Anna
author_sort Starska, Katarzyna
collection PubMed
description Increased glucose uptake mediated by glucose transporters and reliance on glycolysis are common features of malignant cells. Hypoxia-inducible factor-1α supports the adaptation of hypoxic cells by inducing genes related to glucose metabolism. The contribution of glucose transporter (GLUT) and hypoxia-inducible factor-1α (HIF-1α) activity to tumor behavior and their prognostic value in head and neck cancers remains unclear. The aim of this study was to examine the predictive value of GLUT1, GLUT3, and HIF-1α messenger RNA (mRNA)/protein expression as markers of tumor aggressiveness and prognosis in laryngeal cancer. The level of hypoxia/metabolic marker genes was determined in 106 squamous cell laryngeal cancer (SCC) and 73 noncancerous matched mucosa (NCM) controls using quantitative real-time PCR. The related protein levels were analyzed by Western blot. Positive expression of SLC2A1, SLC2A3, and HIF-1α genes was noted in 83.9, 82.1, and 71.7 % of SCC specimens and in 34.4, 59.4, and 62.5 % of laryngeal cancer samples. Higher levels of mRNA/protein for GLUT1 and HIF-1α were noted in SCC compared to NCM (p < 0.05). SLC2A1 was found to have a positive relationship with grade, tumor front grading (TFG) score, and depth and mode of invasion (p < 0.05). SLC2A3 was related to grade and invasion type (p < 0.05). There were also relationships of HIF-1α with pTNM, TFG scale, invasion depth and mode, tumor recurrences, and overall survival (p < 0.05). In addition, more advanced tumors were found to be more likely to demonstrate positive expression of these proteins. In conclusion, the hypoxia/metabolic markers studied could be used as molecular markers of tumor invasiveness in laryngeal cancer.
format Online
Article
Text
id pubmed-4428538
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Springer Netherlands
record_format MEDLINE/PubMed
spelling pubmed-44285382015-05-18 Gene and protein expression of glucose transporter 1 and glucose transporter 3 in human laryngeal cancer—the relationship with regulatory hypoxia-inducible factor-1α expression, tumor invasiveness, and patient prognosis Starska, Katarzyna Forma, Ewa Jóźwiak, Paweł Bryś, Magdalena Lewy-Trenda, Iwona Brzezińska-Błaszczyk, Ewa Krześlak, Anna Tumour Biol Research Article Increased glucose uptake mediated by glucose transporters and reliance on glycolysis are common features of malignant cells. Hypoxia-inducible factor-1α supports the adaptation of hypoxic cells by inducing genes related to glucose metabolism. The contribution of glucose transporter (GLUT) and hypoxia-inducible factor-1α (HIF-1α) activity to tumor behavior and their prognostic value in head and neck cancers remains unclear. The aim of this study was to examine the predictive value of GLUT1, GLUT3, and HIF-1α messenger RNA (mRNA)/protein expression as markers of tumor aggressiveness and prognosis in laryngeal cancer. The level of hypoxia/metabolic marker genes was determined in 106 squamous cell laryngeal cancer (SCC) and 73 noncancerous matched mucosa (NCM) controls using quantitative real-time PCR. The related protein levels were analyzed by Western blot. Positive expression of SLC2A1, SLC2A3, and HIF-1α genes was noted in 83.9, 82.1, and 71.7 % of SCC specimens and in 34.4, 59.4, and 62.5 % of laryngeal cancer samples. Higher levels of mRNA/protein for GLUT1 and HIF-1α were noted in SCC compared to NCM (p < 0.05). SLC2A1 was found to have a positive relationship with grade, tumor front grading (TFG) score, and depth and mode of invasion (p < 0.05). SLC2A3 was related to grade and invasion type (p < 0.05). There were also relationships of HIF-1α with pTNM, TFG scale, invasion depth and mode, tumor recurrences, and overall survival (p < 0.05). In addition, more advanced tumors were found to be more likely to demonstrate positive expression of these proteins. In conclusion, the hypoxia/metabolic markers studied could be used as molecular markers of tumor invasiveness in laryngeal cancer. Springer Netherlands 2014-11-21 /pmc/articles/PMC4428538/ /pubmed/25412955 http://dx.doi.org/10.1007/s13277-014-2838-4 Text en © The Author(s) 2014 https://creativecommons.org/licenses/by/4.0/ Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Research Article
Starska, Katarzyna
Forma, Ewa
Jóźwiak, Paweł
Bryś, Magdalena
Lewy-Trenda, Iwona
Brzezińska-Błaszczyk, Ewa
Krześlak, Anna
Gene and protein expression of glucose transporter 1 and glucose transporter 3 in human laryngeal cancer—the relationship with regulatory hypoxia-inducible factor-1α expression, tumor invasiveness, and patient prognosis
title Gene and protein expression of glucose transporter 1 and glucose transporter 3 in human laryngeal cancer—the relationship with regulatory hypoxia-inducible factor-1α expression, tumor invasiveness, and patient prognosis
title_full Gene and protein expression of glucose transporter 1 and glucose transporter 3 in human laryngeal cancer—the relationship with regulatory hypoxia-inducible factor-1α expression, tumor invasiveness, and patient prognosis
title_fullStr Gene and protein expression of glucose transporter 1 and glucose transporter 3 in human laryngeal cancer—the relationship with regulatory hypoxia-inducible factor-1α expression, tumor invasiveness, and patient prognosis
title_full_unstemmed Gene and protein expression of glucose transporter 1 and glucose transporter 3 in human laryngeal cancer—the relationship with regulatory hypoxia-inducible factor-1α expression, tumor invasiveness, and patient prognosis
title_short Gene and protein expression of glucose transporter 1 and glucose transporter 3 in human laryngeal cancer—the relationship with regulatory hypoxia-inducible factor-1α expression, tumor invasiveness, and patient prognosis
title_sort gene and protein expression of glucose transporter 1 and glucose transporter 3 in human laryngeal cancer—the relationship with regulatory hypoxia-inducible factor-1α expression, tumor invasiveness, and patient prognosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4428538/
https://www.ncbi.nlm.nih.gov/pubmed/25412955
http://dx.doi.org/10.1007/s13277-014-2838-4
work_keys_str_mv AT starskakatarzyna geneandproteinexpressionofglucosetransporter1andglucosetransporter3inhumanlaryngealcancertherelationshipwithregulatoryhypoxiainduciblefactor1aexpressiontumorinvasivenessandpatientprognosis
AT formaewa geneandproteinexpressionofglucosetransporter1andglucosetransporter3inhumanlaryngealcancertherelationshipwithregulatoryhypoxiainduciblefactor1aexpressiontumorinvasivenessandpatientprognosis
AT jozwiakpaweł geneandproteinexpressionofglucosetransporter1andglucosetransporter3inhumanlaryngealcancertherelationshipwithregulatoryhypoxiainduciblefactor1aexpressiontumorinvasivenessandpatientprognosis
AT brysmagdalena geneandproteinexpressionofglucosetransporter1andglucosetransporter3inhumanlaryngealcancertherelationshipwithregulatoryhypoxiainduciblefactor1aexpressiontumorinvasivenessandpatientprognosis
AT lewytrendaiwona geneandproteinexpressionofglucosetransporter1andglucosetransporter3inhumanlaryngealcancertherelationshipwithregulatoryhypoxiainduciblefactor1aexpressiontumorinvasivenessandpatientprognosis
AT brzezinskabłaszczykewa geneandproteinexpressionofglucosetransporter1andglucosetransporter3inhumanlaryngealcancertherelationshipwithregulatoryhypoxiainduciblefactor1aexpressiontumorinvasivenessandpatientprognosis
AT krzeslakanna geneandproteinexpressionofglucosetransporter1andglucosetransporter3inhumanlaryngealcancertherelationshipwithregulatoryhypoxiainduciblefactor1aexpressiontumorinvasivenessandpatientprognosis