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Oligodendrocyte Precursor Cells Synthesize Neuromodulatory Factors
NG2 protein-expressing oligodendrocyte progenitor cells (OPC) are a persisting and major glial cell population in the adult mammalian brain. Direct synaptic innervation of OPC by neurons throughout the brain together with their ability to sense neuronal network activity raises the question of additi...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4429067/ https://www.ncbi.nlm.nih.gov/pubmed/25966014 http://dx.doi.org/10.1371/journal.pone.0127222 |
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author | Sakry, Dominik Yigit, Hatice Dimou, Leda Trotter, Jacqueline |
author_facet | Sakry, Dominik Yigit, Hatice Dimou, Leda Trotter, Jacqueline |
author_sort | Sakry, Dominik |
collection | PubMed |
description | NG2 protein-expressing oligodendrocyte progenitor cells (OPC) are a persisting and major glial cell population in the adult mammalian brain. Direct synaptic innervation of OPC by neurons throughout the brain together with their ability to sense neuronal network activity raises the question of additional physiological roles of OPC, supplementary to generating myelinating oligodendrocytes. In this study we investigated whether OPC express neuromodulatory factors, typically synthesized by other CNS cell types. Our results show that OPC express two well-characterized neuromodulatory proteins: Prostaglandin D2 synthase (PTGDS) and neuronal Pentraxin 2 (Nptx2/Narp). Expression levels of the enzyme PTGDS are influenced in cultured OPC by the NG2 intracellular region which can be released by cleavage and localizes to glial nuclei upon transfection. Furthermore PTGDS mRNA levels are reduced in OPC from NG2-KO mouse brain compared to WT cells after isolation by cell sorting and direct analysis. These results show that OPC can contribute to the expression of these proteins within the CNS and suggest PTGDS expression as a downstream target of NG2 signaling. |
format | Online Article Text |
id | pubmed-4429067 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-44290672015-05-21 Oligodendrocyte Precursor Cells Synthesize Neuromodulatory Factors Sakry, Dominik Yigit, Hatice Dimou, Leda Trotter, Jacqueline PLoS One Research Article NG2 protein-expressing oligodendrocyte progenitor cells (OPC) are a persisting and major glial cell population in the adult mammalian brain. Direct synaptic innervation of OPC by neurons throughout the brain together with their ability to sense neuronal network activity raises the question of additional physiological roles of OPC, supplementary to generating myelinating oligodendrocytes. In this study we investigated whether OPC express neuromodulatory factors, typically synthesized by other CNS cell types. Our results show that OPC express two well-characterized neuromodulatory proteins: Prostaglandin D2 synthase (PTGDS) and neuronal Pentraxin 2 (Nptx2/Narp). Expression levels of the enzyme PTGDS are influenced in cultured OPC by the NG2 intracellular region which can be released by cleavage and localizes to glial nuclei upon transfection. Furthermore PTGDS mRNA levels are reduced in OPC from NG2-KO mouse brain compared to WT cells after isolation by cell sorting and direct analysis. These results show that OPC can contribute to the expression of these proteins within the CNS and suggest PTGDS expression as a downstream target of NG2 signaling. Public Library of Science 2015-05-12 /pmc/articles/PMC4429067/ /pubmed/25966014 http://dx.doi.org/10.1371/journal.pone.0127222 Text en © 2015 Sakry et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Sakry, Dominik Yigit, Hatice Dimou, Leda Trotter, Jacqueline Oligodendrocyte Precursor Cells Synthesize Neuromodulatory Factors |
title | Oligodendrocyte Precursor Cells Synthesize Neuromodulatory Factors |
title_full | Oligodendrocyte Precursor Cells Synthesize Neuromodulatory Factors |
title_fullStr | Oligodendrocyte Precursor Cells Synthesize Neuromodulatory Factors |
title_full_unstemmed | Oligodendrocyte Precursor Cells Synthesize Neuromodulatory Factors |
title_short | Oligodendrocyte Precursor Cells Synthesize Neuromodulatory Factors |
title_sort | oligodendrocyte precursor cells synthesize neuromodulatory factors |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4429067/ https://www.ncbi.nlm.nih.gov/pubmed/25966014 http://dx.doi.org/10.1371/journal.pone.0127222 |
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