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Magnitude and kinetics of multifunctional CD4(+) and CD8β(+) T cells in pigs infected with swine influenza A virus

Although swine are natural hosts for influenza A viruses, the porcine T-cell response to swine influenza A virus (FLUAVsw) infection has been poorly characterized so far. We have studied Ki-67 expression and FLUAVsw-specific production of IFN-γ, TNF-α and IL-2 in CD4(+) and CD8β(+) T cells isolated...

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Autores principales: Talker, Stephanie C, Koinig, Hanna C, Stadler, Maria, Graage, Robert, Klingler, Eva, Ladinig, Andrea, Mair, Kerstin H, Hammer, Sabine E, Weissenböck, Herbert, Dürrwald, Ralf, Ritzmann, Mathias, Saalmüller, Armin, Gerner, Wilhelm
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4429459/
https://www.ncbi.nlm.nih.gov/pubmed/25971313
http://dx.doi.org/10.1186/s13567-015-0182-3
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author Talker, Stephanie C
Koinig, Hanna C
Stadler, Maria
Graage, Robert
Klingler, Eva
Ladinig, Andrea
Mair, Kerstin H
Hammer, Sabine E
Weissenböck, Herbert
Dürrwald, Ralf
Ritzmann, Mathias
Saalmüller, Armin
Gerner, Wilhelm
author_facet Talker, Stephanie C
Koinig, Hanna C
Stadler, Maria
Graage, Robert
Klingler, Eva
Ladinig, Andrea
Mair, Kerstin H
Hammer, Sabine E
Weissenböck, Herbert
Dürrwald, Ralf
Ritzmann, Mathias
Saalmüller, Armin
Gerner, Wilhelm
author_sort Talker, Stephanie C
collection PubMed
description Although swine are natural hosts for influenza A viruses, the porcine T-cell response to swine influenza A virus (FLUAVsw) infection has been poorly characterized so far. We have studied Ki-67 expression and FLUAVsw-specific production of IFN-γ, TNF-α and IL-2 in CD4(+) and CD8β(+) T cells isolated from piglets that had been intratracheally infected with a H1N2 FLUAVsw isolate. IFN-γ(+)TNF-α(+)IL-2(+) multifunctional CD4(+) T cells were present in the blood of all infected animals at one or two weeks after primary infection and their frequency increased in four out of six animals after homologous secondary infection. These cells produced higher amounts of IFN-γ, TNF-α and IL-2 than did CD4(+) T cells that only produced a single cytokine. The vast majority of cytokine-producing CD4(+) T cells expressed CD8α, a marker associated with activation and memory formation in porcine CD4(+) T cells. Analysis of CD27 expression suggested that FLUAVsw-specific CD4(+) T cells included both central memory and effector memory populations. Three out of six animals showed a strong increase of Ki-67(+)perforin(+) CD8β(+) T cells in blood one week post infection. Blood-derived FLUAVsw-specific CD8β(+) T cells could be identified after an in vitro expansion phase and were multifunctional in terms of CD107a expression and co-production of IFN-γ and TNF-α. These data show that multifunctional T cells are generated in response to FLUAVsw infection of pigs, supporting the idea that T cells contribute to the efficient control of infection. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13567-015-0182-3) contains supplementary material, which is available to authorized users.
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spelling pubmed-44294592015-05-14 Magnitude and kinetics of multifunctional CD4(+) and CD8β(+) T cells in pigs infected with swine influenza A virus Talker, Stephanie C Koinig, Hanna C Stadler, Maria Graage, Robert Klingler, Eva Ladinig, Andrea Mair, Kerstin H Hammer, Sabine E Weissenböck, Herbert Dürrwald, Ralf Ritzmann, Mathias Saalmüller, Armin Gerner, Wilhelm Vet Res Research Article Although swine are natural hosts for influenza A viruses, the porcine T-cell response to swine influenza A virus (FLUAVsw) infection has been poorly characterized so far. We have studied Ki-67 expression and FLUAVsw-specific production of IFN-γ, TNF-α and IL-2 in CD4(+) and CD8β(+) T cells isolated from piglets that had been intratracheally infected with a H1N2 FLUAVsw isolate. IFN-γ(+)TNF-α(+)IL-2(+) multifunctional CD4(+) T cells were present in the blood of all infected animals at one or two weeks after primary infection and their frequency increased in four out of six animals after homologous secondary infection. These cells produced higher amounts of IFN-γ, TNF-α and IL-2 than did CD4(+) T cells that only produced a single cytokine. The vast majority of cytokine-producing CD4(+) T cells expressed CD8α, a marker associated with activation and memory formation in porcine CD4(+) T cells. Analysis of CD27 expression suggested that FLUAVsw-specific CD4(+) T cells included both central memory and effector memory populations. Three out of six animals showed a strong increase of Ki-67(+)perforin(+) CD8β(+) T cells in blood one week post infection. Blood-derived FLUAVsw-specific CD8β(+) T cells could be identified after an in vitro expansion phase and were multifunctional in terms of CD107a expression and co-production of IFN-γ and TNF-α. These data show that multifunctional T cells are generated in response to FLUAVsw infection of pigs, supporting the idea that T cells contribute to the efficient control of infection. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13567-015-0182-3) contains supplementary material, which is available to authorized users. BioMed Central 2015-05-14 2015 /pmc/articles/PMC4429459/ /pubmed/25971313 http://dx.doi.org/10.1186/s13567-015-0182-3 Text en © Talker et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Talker, Stephanie C
Koinig, Hanna C
Stadler, Maria
Graage, Robert
Klingler, Eva
Ladinig, Andrea
Mair, Kerstin H
Hammer, Sabine E
Weissenböck, Herbert
Dürrwald, Ralf
Ritzmann, Mathias
Saalmüller, Armin
Gerner, Wilhelm
Magnitude and kinetics of multifunctional CD4(+) and CD8β(+) T cells in pigs infected with swine influenza A virus
title Magnitude and kinetics of multifunctional CD4(+) and CD8β(+) T cells in pigs infected with swine influenza A virus
title_full Magnitude and kinetics of multifunctional CD4(+) and CD8β(+) T cells in pigs infected with swine influenza A virus
title_fullStr Magnitude and kinetics of multifunctional CD4(+) and CD8β(+) T cells in pigs infected with swine influenza A virus
title_full_unstemmed Magnitude and kinetics of multifunctional CD4(+) and CD8β(+) T cells in pigs infected with swine influenza A virus
title_short Magnitude and kinetics of multifunctional CD4(+) and CD8β(+) T cells in pigs infected with swine influenza A virus
title_sort magnitude and kinetics of multifunctional cd4(+) and cd8β(+) t cells in pigs infected with swine influenza a virus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4429459/
https://www.ncbi.nlm.nih.gov/pubmed/25971313
http://dx.doi.org/10.1186/s13567-015-0182-3
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