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Monitoring protein phosphorylation by acrylamide pendant Phos-Tag™ in various plants
The aim of the present study is to rationalize acrylamide pendant Phos-Tag™ in-gel discrimination of phosphorylated and non-phosphorylated plant protein species with standard immunoblot analysis, and optimize sample preparation, efficient electrophoretic separation and transfer. We tested variants o...
Autores principales: | Bekešová, Slávka, Komis, George, Křenek, Pavel, Vyplelová, Petra, Ovečka, Miroslav, Luptovčiak, Ivan, Illés, Peter, Kuchařová, Anna, Šamaj, Jozef |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4429547/ https://www.ncbi.nlm.nih.gov/pubmed/26029234 http://dx.doi.org/10.3389/fpls.2015.00336 |
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