A hybrid-membrane migration method to isolate high-purity adipose-derived stem cells from fat tissues

Human adipose-derived stem cells (hADSCs) exhibit heterogeneous characteristics, indicating various genotypes and differentiation abilities. The isolated hADSCs can possess different purity levels and divergent properties depending on the purification methods used. We developed a hybrid-membrane mig...

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Autores principales: Higuchi, Akon, Wang, Ching-Tang, Ling, Qing-Dong, Lee, Henry Hsin-chung, Kumar, S. Suresh, Chang, Yung, Alarfaj, Abdullah A., Munusamy, Murugan A., Hsu, Shih-Tien, Wu, Gwo-Jang, Umezawa, Akihiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4429558/
https://www.ncbi.nlm.nih.gov/pubmed/25970301
http://dx.doi.org/10.1038/srep10217
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author Higuchi, Akon
Wang, Ching-Tang
Ling, Qing-Dong
Lee, Henry Hsin-chung
Kumar, S. Suresh
Chang, Yung
Alarfaj, Abdullah A.
Munusamy, Murugan A.
Hsu, Shih-Tien
Wu, Gwo-Jang
Umezawa, Akihiko
author_facet Higuchi, Akon
Wang, Ching-Tang
Ling, Qing-Dong
Lee, Henry Hsin-chung
Kumar, S. Suresh
Chang, Yung
Alarfaj, Abdullah A.
Munusamy, Murugan A.
Hsu, Shih-Tien
Wu, Gwo-Jang
Umezawa, Akihiko
author_sort Higuchi, Akon
collection PubMed
description Human adipose-derived stem cells (hADSCs) exhibit heterogeneous characteristics, indicating various genotypes and differentiation abilities. The isolated hADSCs can possess different purity levels and divergent properties depending on the purification methods used. We developed a hybrid-membrane migration method that purifies hADSCs from a fat tissue solution with extremely high purity and pluripotency. A primary fat-tissue solution was permeated through the porous membranes with a pore size from 8 to 25 μm, and the membranes were incubated in cell culture medium for 15-18 days. The hADSCs that migrated from the membranes contained an extremely high percentage (e.g., >98%) of cells positive for mesenchymal stem cell markers and showed almost one order of magnitude higher expression of some pluripotency genes (Oct4, Sox2, Klf4 and Nanog) compared with cells isolated using the conventional culture method.
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spelling pubmed-44295582015-05-21 A hybrid-membrane migration method to isolate high-purity adipose-derived stem cells from fat tissues Higuchi, Akon Wang, Ching-Tang Ling, Qing-Dong Lee, Henry Hsin-chung Kumar, S. Suresh Chang, Yung Alarfaj, Abdullah A. Munusamy, Murugan A. Hsu, Shih-Tien Wu, Gwo-Jang Umezawa, Akihiko Sci Rep Article Human adipose-derived stem cells (hADSCs) exhibit heterogeneous characteristics, indicating various genotypes and differentiation abilities. The isolated hADSCs can possess different purity levels and divergent properties depending on the purification methods used. We developed a hybrid-membrane migration method that purifies hADSCs from a fat tissue solution with extremely high purity and pluripotency. A primary fat-tissue solution was permeated through the porous membranes with a pore size from 8 to 25 μm, and the membranes were incubated in cell culture medium for 15-18 days. The hADSCs that migrated from the membranes contained an extremely high percentage (e.g., >98%) of cells positive for mesenchymal stem cell markers and showed almost one order of magnitude higher expression of some pluripotency genes (Oct4, Sox2, Klf4 and Nanog) compared with cells isolated using the conventional culture method. Nature Publishing Group 2015-05-13 /pmc/articles/PMC4429558/ /pubmed/25970301 http://dx.doi.org/10.1038/srep10217 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Higuchi, Akon
Wang, Ching-Tang
Ling, Qing-Dong
Lee, Henry Hsin-chung
Kumar, S. Suresh
Chang, Yung
Alarfaj, Abdullah A.
Munusamy, Murugan A.
Hsu, Shih-Tien
Wu, Gwo-Jang
Umezawa, Akihiko
A hybrid-membrane migration method to isolate high-purity adipose-derived stem cells from fat tissues
title A hybrid-membrane migration method to isolate high-purity adipose-derived stem cells from fat tissues
title_full A hybrid-membrane migration method to isolate high-purity adipose-derived stem cells from fat tissues
title_fullStr A hybrid-membrane migration method to isolate high-purity adipose-derived stem cells from fat tissues
title_full_unstemmed A hybrid-membrane migration method to isolate high-purity adipose-derived stem cells from fat tissues
title_short A hybrid-membrane migration method to isolate high-purity adipose-derived stem cells from fat tissues
title_sort hybrid-membrane migration method to isolate high-purity adipose-derived stem cells from fat tissues
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4429558/
https://www.ncbi.nlm.nih.gov/pubmed/25970301
http://dx.doi.org/10.1038/srep10217
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